A5015663431- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Renal cell carcinoma treatment
- Renal and related cancers
- Kidney Stones and Urolithiasis Treatments
- Pediatric Urology and Nephrology Studies
- Reproductive Health and Technologies
- Ureteral procedures and complications
- Urological Disorders and Treatments
- Renal and Vascular Pathologies
- Testicular diseases and treatments
- Bladder and Urothelial Cancer Treatments
- 3D Printing in Biomedical Research
- Ovarian function and disorders
- Urinary and Genital Oncology Studies
- Male Reproductive Health Studies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Urinary Bladder and Prostate Research
- Urologic and reproductive health conditions
- Genital Health and Disease
- Tissue Engineering and Regenerative Medicine
- Organ Donation and Transplantation
- Cancer Genomics and Diagnostics
- Renal Diseases and Glomerulopathies
- Hormonal and reproductive studies
Yokohama City University
2016-2025
Yokohama City University Hospital
2023-2025
Nagoya University
2024
Yokohama City University Medical Center
2020-2023
Higashi Osaka City General Hospital
2020
Synthetic Biologics (United States)
2018
Kookmin University
2018
Fujisawa City Hospital
2011-2012
Saiseikai Central Hospital
2006
Abstract In contrast to cell cultures, particularly lines, tissues or organs removed from the body cannot be maintained for long in any culture conditions. Although it is apparent that vivo regional homeostasis facilitated by microvascular system, mimicking such a system ex difficult and has not been proved effective. Using of mouse spermatogenesis, we addressed this issue devised simple microfluidic device which porous membrane separates tissue flowing medium, conceptually imitating...
Three-dimensional aggregation and organ culture methods are critical for recreating in vivo cellular phenomena outside the body. Previously, we used conventional gas liquid interphase method to induce complete mouse spermatogenesis. After incorporating microfluidic systems, achieved a significant increase efficiency duration of One major drawbacks preventing popularization microfluidics, however, is use power-pump generate medium flow. In this study, produced pumpless device using...
Objective In December 2021, enfortumab vedotin (EV), an antibody‐drug conjugate directed against nectin‐4, was approved in Japan as a new treatment after platinum‐containing chemotherapy and PD‐1/PD‐L1 inhibitors. This study evaluated, using real‐world data, the efficacy safety of EV therapy patients with metastatic urothelial carcinoma (mUC). Materials methods Fifty‐five mUC who discontinued pembrolizumab due to disease progression between June 2018 April 2023 at Yokohama City University...
It is known that cells of testis tissues in fetal or neonatal periods have the ability to reconstruct testicular architecture even after dissociation into single cells. This ability, however, has not been demonstrated effectively vitro. In our present study, we succeeded reconstructing seminiferous tubules vitro which supported spermatogenesis meiotic phase. Testis mice were dissociated enzymatically The formed aggregates suspension culture and transferred surface agarose gel continue with a...
Research on in vitro spermatogenesis is important for elucidating the spermatogenic mechanism. We previously developed an organ culture method which can support from spermatogonial stem cells up to sperm formation using immature mouse testis tissues. In this study, we examined whether it also applicable mature tissues of adult mice. used two lines transgenic mice, Acrosin-GFP and Gsg2-GFP, carry marker GFP gene specific meiotic haploid cells, respectively. Testis tissue fragments aged 4 29...
Male infertility is most commonly caused by spermatogenic defects or insufficiencies, the majority of which are as yet cureless. Recently, we succeeded in cultivating mouse testicular tissues for producing fertile sperm from spermatogonial stem cells. Here, show that one severe types defect mutant can be treated culture method without any genetic manipulations. The Sl/Sl d used a model such male infertility. testis has only primitive spermatogonia germ cells, lacking sign spermatogenesis...
We previously reported the successful induction and completion of mouse spermatogenesis by culturing neonatal testis tissues. The culture medium consisted α-minimum essential (α-MEM), supplemented with Knockout serum replacement (KSR) or AlbuMAX, neither which were defined chemically. In this study, we formulated a chemically (CDM) that can induce under organ conditions. It was found bovine albumin (BSA) purified through three different procedures had effects on spermatogenesis. also...
Complete endophytic renal tumors (CERTs) are the most challenging for robot-assisted partial nephrectomy (RAPN). This study aimed to determine impact of CERT on outcomes RAPN.
Lower urinary tract symptoms (LUTS) significantly affect patient quality of life. Treatment options for bladder outlet obstruction (BOO) due to benign prostatic hyperplasia (BPH) (a common cause LUTS) are insufficient relieve discomfort. As the incidence BPH is increasing, new pharmacological targets LUTS treatment required. Corticotropin-releasing hormone (CRH) a neuropeptide that controls normal micturition in rodents. Herein, we investigated role spinal CRH regulating sham and BOO rats,...
Coronavirus disease-2019 (COVID-19) is associated with a wide range of post-acute sequelae. The prevalence erectile dysfunction (ED) that developed after COVID-19 and the underlying factors were analyzed based on questionnaire survey, Recovery Study II in Japan. A case-control study was conducted those or without ED one two years hospitalized between March September 2021. Six hundred nine Japanese men, median age 48 years, analyzed. During period, 116 subjects (19.0%) had dysfunction....
Organ culture experiments can be hampered by central degeneration or necrosis due to the inadequate permeation of oxygen and nutrients, which deteriorates function growth cultured tissues. In current study, we aimed overcome this limitation organ through spreading tissue two dimensionally on an agarose gel stand molding into a disc shape placing ceiling polydimethylsiloxane (PDMS) chip, is highly permeable. By this, every part receive sufficient supply PDMS as well nutrients below. This...
Mouse in vitro spermatogenesis is possible with classical organ culture methods, by placing the testis tissue at interphase between medium and air. In this condition, however, a piece tends to round up be compact, whose central region suffers from shortage of nutrients oxygen. study, authors improved condition spreading each thin flat, which they were able get better access oxygen nutrients.Immature mouse tissues placed on agarose gel block forced spread flat covering polydimethylsiloxane...
In vitro mouse spermatogenesis using a classical organ culture method became possible by supplementing basal medium with only the product of bovine serum albumin purified chromatography (AlbuMAX), which indicated that AlbuMAX contained every chemical factor necessary for spermatogenesis. However, since identity these factors was unclear, improvements in media and our understanding nutritional signal substances required were hindered. present study, chemically defined (CDM) without used to...
Our understanding of how each hereditary kidney cancer adapts to its tissue microenvironment is incomplete. Here, we present single-cell transcriptomes 108,342 cells from patient specimens including six cancers. The displayed distinct characteristics the cell origin and unique for cancer. Of note, leiomyomatosis renal carcinoma (HLRCC)-associated retained some proximal tubules, which were completely lost in lymph node metastases as an avascular tumor with suppressed T TREM2-high macrophages,...