A5044168723- Salivary Gland Disorders and Functions
- Salivary Gland Tumors Diagnosis and Treatment
- Mesenchymal stem cell research
- Head and Neck Cancer Studies
- Ocular Surface and Contact Lens
- Systemic Lupus Erythematosus Research
- Cancer Immunotherapy and Biomarkers
- Proteoglycans and glycosaminoglycans research
- Atherosclerosis and Cardiovascular Diseases
- Vasculitis and related conditions
- Cancer Cells and Metastasis
- Systemic Sclerosis and Related Diseases
- Oral health in cancer treatment
- Wnt/β-catenin signaling in development and cancer
- Immunotherapy and Immune Responses
- Connective Tissue Growth Factor Research
- Immune Response and Inflammation
- Galectins and Cancer Biology
- Oral Health Pathology and Treatment
- Studies on Chitinases and Chitosanases
- Pluripotent Stem Cells Research
- Dermatoglyphics and Human Traits
- Reproductive tract infections research
- Otitis Media and Relapsing Polychondritis
- Vascular Anomalies and Treatments
University of Groningen
2013-2024
University Medical Center Groningen
2011-2024
Autoimmune Technologies (United States)
2022
National and Kapodistrian University of Athens
2022
Universitat de Barcelona
2022
Research Institute of General Pathology and Pathophysiology, the Russian Academy of Medical Sciences
2022
Center for Rheumatology
2022
Queen Mary University of London
2022
Dialyse Centrum Groningen
2006-2021
King's College London
2011
Adult stem cells are the ultimate source for replenishment of salivary gland (SG) tissue. Self-renewal ability is dependent on extrinsic niche signals that have not been unraveled SG. The ductal compartment in SG has identified as location harboring cells. Here, we report rare EpCAM(+) express nuclear β-catenin, indicating active Wnt signaling. In cell culture experiments, EpCAM(high) respond potently to stimulating self-renewal and long-term expansion organoids, containing all...
Abstract Adult stem cells are often touted as therapeutic agents in the regenerative medicine field, however data detailing both engraftment and functional capabilities of solid tissue derived human adult epithelial is scarce. Here we show isolation salivary gland (SG) stem/progenitor demonstrate at single cell level vitro self-renewal differentiation into multilineage organoids. We also vivo functionality, long-term engraftment, restoration a xenotransplantation model. Indeed, transplanted...
Avoiding irradiation of the region parotid gland containing stem cells reduces risk xerostomia (dry mouth).
While all salivary glands (SGs) can be involved in primary Sjögren's syndrome (pSS), their respective role pathogenesis remains unclear. Our objective was to assess immunopathway activation paired parotid and labial gland tissue from biopsy-positive biopsy-negative pSS non-SS sicca patients.
Inter-individual variation largely influences disease susceptibility, as well response to therapy. In a clinical context, the optimal treatment of should consider inter-individual and formulate tailored decisions at an individual level. recent years, emerging organoid technologies promise capture part individual's phenotypic variability prove helpful in providing clinically relevant molecular insights. Organoids are stem cell-derived 3-dimensional models that contain multiple cell types can...
Objective A major characteristic of the autoimmune disease primary Sjögren's syndrome ( SS ) is salivary gland SG hypofunction. The inability resident stem cells SGSC s) to maintain homeostasis and saliva production has never been explained limits our comprehension mechanisms underlying . present study was undertaken investigate role in hyposalivation SS. Methods s were isolated from parotid biopsy samples controls patients classified as having or incomplete , according American College...
In primary Sjögren's syndrome (pSS), FcRL4+ B cells are present in inflamed salivary gland tissue, within or close proximity to ductal epithelium. FcRL4 is also expressed by nearly all pSS-related mucosa-associated lymphoid tissue (MALT) cell lymphomas, linking expression lymphomagenesis. Whether glandular pathogenic, how these originate, and they functionally differ from FcRL4- pSS unclear. This study aimed investigate the phenotype function of periphery parotid patients with pSS. First,...
Macrophages mediate inflammation, angiogenesis, and tissue destruction in giant cell arteritis (GCA). Serum levels of the macrophage-associated protein YKL-40 (chitinase 3-like 1), previously linked to angiogenesis remodeling, remain elevated GCA despite glucocorticoid treatment. This study was undertaken investigate contribution vasculopathy GCA.Immunohistochemistry performed on temporal artery biopsy specimens (n = 12) aortas 10) for detection YKL-40, its receptor interleukin-13 α2...
Salivary gland (SG) progenitor cells (SGPCs) maintain SG homeostasis. We have previously shown that in primary Sjögren's syndrome (pSS), SGPCs are likely to be senescent, and may underpin dysfunction. This study assessed the extent of senescence a SGPC niche pSS patients' SGs, its correlation with functional clinical parameters.The expression p16 p21 as markers both total epithelium (basal striated duct cells, BSD) was examined SGs (n = 35), incomplete 13) (patients some signs pSS, but not...
Mature salivary glands of both human and mouse origin comprise a minimum five cell types, each which facilitates the production excretion saliva into oral cavity. Serous mucous acinar cells are protein producing factories gland respectively, represent production. Once synthesised, various enzymatic other proteinaceous components secreted through series ductal bearing epithelial-type morphology, until eventual expulsion one major duct cavity mouth. The composition is also modified by during...
Abstract Objective Hyposalivation‐related xerostomia is an irreversible, untreatable, and frequent condition after radiotherapy for head neck cancer. Stem cell therapy attractive option of treatment, but demands knowledge stem functioning. Therefore, we aimed to develop a murine parotid gland organoid model explore radiation response cells in vitro. Materials Methods Single derived from organoids were passaged Matrigel with defined medium assess self‐renewal differentiation potential....
Giant cell arteritis (GCA) affects almost exclusively individuals above 50 years old, suggesting a role of aging-related changes such as cellular senescence in its pathobiology. The kinases p21(WAF1/CIP1) and p16/INK4A play key roles 2 distinct pathways leading to senescence. proinflammatory molecules interleukin-6 (IL-6) granulocyte-macrophage colony-stimulating factor (GM-CSF), which are components the senescence-associated secretory phenotype (SASP), effective targets treatment GCA. Here,...
High label uptake in 68 Ga-PSMA-11 PET/CT recently identified a bilateral nasopharyngeal structure as salivary gland (SG)-like additional 'area of interest', to be considered conditions affecting SGs. These structures were termed 'tubarial glands'. We aimed further characterize their histological and immunohistochemical position compared established SGs.Tubarial tissue was with parotid, submandibular, sublingual, palatal labial SGs using immunohistological techniques.Expression acinar...
The oral cavity is a critical barrier with immunosurveillance capabilities. A detailed understanding of its cellular, molecular, and spatial architecture essential for advancing precision medicine across aerodigestive tissues. Here, we present the first integrated atlas human adult craniofacial tissues, derived from single-cell RNA sequencing ~250,000 cells 70 samples 13 niches, including salivary glands mucosae. Complementary atlases using 40-plex protein 300-plex transcriptomics panels...
The autoimmune disease primary Sjögren's syndrome (pSS) is characterized by hypofunction of the salivary glands (SGs), cause which not correlated to lymphocytic SG infiltration, as prevailing dogma often states. We knocked out NF-κB proinflammatory pathway inhibitor A20 in keratin14+ epithelial cells, investigate if immune activated cells are capable initiating pSS hallmarks. show that can T cell dominated leukocytic infiltration and foci development SGs, reflecting early clinical picture....
Background Salivary glands (SGs) can be damaged by immune checkpoint inhibitor (ICI) therapy. In patients with ICI-induced SG dysfunction, 60% progress to fulfill classification criteria for primary Sjögren's syndrome (pSS), owing foci in SGs and/or anti-SSA autoantibody positivity. We report the tissue analysis of a patient dysfunction after treatment programmed death ligand-1 (PD-L1) inhibitor, compared that dry mouth ('sicca') control and pSS patient. Case The received PD-L1 durvalumab...