J.U. Mondal
A5107992610- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Photosynthetic Processes and Mechanisms
- Fibroblast Growth Factor Research
- Cancer-related Molecular Pathways
- Genomics and Phylogenetic Studies
- Metal complexes synthesis and properties
- Enzyme Structure and Function
- Advanced Electron Microscopy Techniques and Applications
- Cancer Research and Treatments
- Solid-state spectroscopy and crystallography
- ATP Synthase and ATPases Research
- Polyoxometalates: Synthesis and Applications
- Molecular Sensors and Ion Detection
- Bacteriophages and microbial interactions
- Cancer, Hypoxia, and Metabolism
- Magnetism in coordination complexes
- Electrochemical sensors and biosensors
- Plant Diversity and Evolution
- nanoparticles nucleation surface interactions
- Advanced X-ray Imaging Techniques
- Metal-Catalyzed Oxygenation Mechanisms
- Spectroscopy and Quantum Chemical Studies
- Porphyrin and Phthalocyanine Chemistry
- Metalloenzymes and iron-sulfur proteins
University of Tennessee at Knoxville
1987-2021
Indiana University – Purdue University Indianapolis
1989
Oak Ridge National Laboratory
1987
Abstract The world’s first superconducting megahertz repetition rate hard X-ray free-electron laser (XFEL), the European XFEL, began operation in 2017, featuring a unique pulse train structure with 886 ns between pulses. With its rapid rate, XFEL may alleviate some of increasing demand for beamtime, particularly membrane protein serial femtosecond crystallography (SFX), leveraging orders-of-magnitude faster data collection. Here, we report SFX experiment, where determined 2.9 Å-resolution...
Ferredoxin (Fd) is a small soluble iron-sulfur protein essential in almost all oxygenic photosynthetic organisms. It contains single [2Fe-2S] cluster coordinated by four cysteine ligands. accepts electrons from the stromal surface of PSI and facilitates transfer to myriad acceptors involved diverse metabolic processes, including generation NADPH via Fd-NADP-reductase, cyclic electron transport for ATP synthesis, nitrate reduction, nitrite reductase, sulfite hydrogenase other reductive...
Photosystem I (PSI) is one of two photosystems involved in oxygenic photosynthesis. PSI cyanobacteria exists monomeric, trimeric, and tetrameric forms, contrast to the strictly monomeric form plants algae. The organization raises questions about its structural, physiological, evolutionary significance. Here we report ∼3.72 Å resolution cryo-electron microscopy structure from thermophilic, unicellular cyanobacterium Chroococcidiopsis sp. TS-821. resolves 44 subunits 448 cofactor molecules. We...
Serial femtosecond crystallography (SFX) is a powerful technique that exploits X-ray free-electron lasers to determine the structure of macromolecules at room temperature. Despite impressive exposition structural details with this novel crystallographic approach, methods currently available introduce crystals into path beam sometimes exhibit serious drawbacks. Samples requiring liquid injection crystal slurries consume large quantities (at times up gram protein per data set), may not be...
An amendment to this paper has been published and can be accessed via a link at the top of paper.
Abstract Photosystem I (PSI) is one of two the photosystems involved in oxygenic photosynthesis. PSI cyanobacteria exists monomeric, trimeric, and tetrameric forms, which contrast to strictly monomeric form plants algae. The organization raises questions about its structural, physiological, evolutional significance. Here we report ~3.9 Å resolution cryo-EM structure from thermophilic, unicellular cyanobacterium Chroococcidiopsis sp. TS-821. resolves all 44 subunits 448 cofactor molecules. We...