A5085101086- Lipid metabolism and biosynthesis
- Adenosine and Purinergic Signaling
- Cholesterol and Lipid Metabolism
- Liver Disease Diagnosis and Treatment
- Cancer, Lipids, and Metabolism
- Metabolomics and Mass Spectrometry Studies
- interferon and immune responses
- Carbon Dioxide Capture Technologies
- Endoplasmic Reticulum Stress and Disease
- Ocular Surface and Contact Lens
- Cancer Mechanisms and Therapy
- Mass Spectrometry Techniques and Applications
- Retinoids in leukemia and cellular processes
- Neuropeptides and Animal Physiology
- Drug Transport and Resistance Mechanisms
- Pancreatic function and diabetes
- Child Nutrition and Feeding Issues
- Educational theories and practices
- Advanced Proteomics Techniques and Applications
- MicroRNA in disease regulation
- Bee Products Chemical Analysis
- Higher Education Teaching and Evaluation
- Membrane-based Ion Separation Techniques
- Membrane Separation and Gas Transport
- Mechanisms of cancer metastasis
University of the Basque Country
2014-2023
E2F transcription factors are key regulators of the cell cycle although relative contribution each member in regulating cellular proliferation is still poorly defined. Present evidence suggests that E2F2 may act both as a suppressor and promoter proliferation, depending on context. We used loss-of-function mutant mouse model to investigate function liver regeneration after partial hepatectomy, paradigm cell-cycle progression. Liver mass recovery histology were examined over 9 days 70%...
Lipid imaging mass spectrometry (LIMS) has been tested in several pathological contexts, demonstrating its ability to segregate and isolate lipid signatures complex tissues, thanks the technique's spatial resolution. However, it cannot yet compete with superior identification power of high-performance liquid chromatography coupled (HPLC-MS), therefore, very often, latter is used refine assignment species detected by LIMS. Also, not clear if differences sensitivity resolution between two...
For many years, traditional histology has been the gold standard for diagnosis of diseases. However, alternative and powerful techniques have appeared in recent years that complement information extracted from a tissue section. One most promising is imaging mass spectrometry applied to lipidomics. Here, we demonstrate capabilities this technique highlight architectural features human kidney at spatial resolution 10 μm. Our data up seven different segments nephron interstitial can be readily...
The mammalian liver, a key organ in lipid homeostasis, can accumulate increased amounts of lipids certain physiological conditions including liver regeneration. Lipid droplets (LD), the storage organelles cytoplasm, are composed core neutral (mainly triacylglycerols and cholesteryl esters) surrounded by monolayer phospholipids cholesterol with associated proteins. It is recognized that LD composition cell- environment-specific enables to carry out specific functions, but few descriptive...
High-fat diet (HFD) feeding or leptin-deficient mice are extensively used as models resembling features of human nonalcoholic fatty liver disease (NAFLD). The concurrence experimental factors fat content and source total caloric intake leads to prominent differences in the development hepatic steatosis related disturbances. In this work, we characterized lipid accumulation induced by HFD wild-type (WT) ob/ ob with purpose differentiating adaptations from those specific increased overfeeding...
Adenosine is readily available to the glandular epithelium of stomach. Formed continuously in intracellular and extracellular locations, it notably produced from ATP released enteric cotransmission. analogs modulate chloride secretion gastric glands activate acid isolated parietal cells through A2B adenosine receptor (A2BR) binding. A functional link between surface A2BR deaminase (ADA) was found cells, but whether this connection a general feature mucosa unknown. Here we examine expressed...
Adenosine modulates different functional activities in many cells of the gastrointestinal tract; some them are believed to be mediated by interaction with its four G protein-coupled receptors. The renewed interest adenosine A 2B receptor (A2BR) subtype can traced studies which introduction new genetic and chemical tools has widened pharmacological structural knowledge this as well potential therapeutic use cancer inflammation- or hypoxia-related pathologies. In acid-secreting parietal...
The innate immune response to pathogens during the acute phase includes lipid metabolism adaptations. Hepatic triacylglycerol (TG) and cholesteryl ester (CE) storage in mobilization from droplets (LDs) respond metabolic changes under control of liver X receptor (LXR) transactivation cytokine transduction. To evaluate whether alterations these mechanisms have an impact adaptive endotoxemia, we analysed lipopolysaccharide (LPS)-treated ob/ob mice, which show altered responses a higher...
L. Saenz Del BurgoC. MiguelezG. PurasT. MoreraJ. CirizaA. FernándezM. TorrecillaI. FernándezE. ErasoE. SevillanoE. MateoM.P. PortilloE. EchevarriaS. BasurtoS. CariñanosA. UlibarriG. RubialesH. GurruchagaG. OriveJ.L. PedrazA. AcarreguiO. CasisM. GallegoY. RuedaA.M. Ochoa de Retana
Background: Transcriptomic and lipidomic dual analyses usually initiate with independent extractive procedures. That entails a difficulty in aligning results from both omics platforms, especially the case of highly heterogeneous tissues, such as kidney. Methods: Bligh Dyer lipid extraction was performed using rat kidney homogenates prepared PBS or commercially available Tri-reagent used for RNA extraction. Samples were analyzed by ultrahigh performance liquid chromatography-mass spectrometry...
Lipido-tantak klasikoki lipido-biltegi inertetzat hartu badira ere, gaur egun benetako organulutzat hartzen dira, beren konplexutasunagatik eta metabolismoan zelulen beste fenomenoetan dituzten zereginengatik. Erretikulu endoplasmatikoan sortu bertatik askatu ondoren, bizitzan zehar hazi egiten lipidoen proteinen transferentzia dute, fusio prozesuetan dute parte elkarrekintza espezifikoak izaten dituzte organuluekin. Gordetzen lipidoak erregai zein molekula batzuen aitzindari gisa erabiltzen...
In the context of a study involvement SND1 (also known as coactivator p100) in biliary lipid secretion by primary rat hepatocytes, first-generation adenoviral vectors were used to promote overexpression and underexpression protein SND1. Although differential expression did not result significant changes processes studied, some effects infection itself observed. particular, infected hepatocytes showed higher intracellular taurocholate accumulation capacity. Additionally, small heterodimer...
Heste mehera isurtzen diren behazun-gatzek lipidoen digestioan eta xurgapenean laguntzen dute. Behazun-gatzak hepatozitoek sintetizatzen dituzte kolesterola erabiliz; beren izaera anfipatikoa egitura molekularra direla-eta, detergente gisa jokatzen dute, beraz toxikoak izan daitezke kontzentrazio handietan. Hori horrela izanda, sintesia garraioa oso modu finean erregulatu behar dira, bai gibelean behazun-gatzen garraioan parte hartzen duten beste ehunetan. Lan honetan gainbegirada bat egin...