A5041175917- Phytochemicals and Antioxidant Activities
- Metabolomics and Mass Spectrometry Studies
- Mass Spectrometry Techniques and Applications
- Fermentation and Sensory Analysis
- Analytical Chemistry and Chromatography
- Phytochemistry and Biological Activities
- Natural product bioactivities and synthesis
- Advanced Proteomics Techniques and Applications
- Ocular Surface and Contact Lens
- Horticultural and Viticultural Research
- Protein Kinase Regulation and GTPase Signaling
- Cholesterol and Lipid Metabolism
- Estrogen and related hormone effects
- Antioxidant Activity and Oxidative Stress
- Nuclear Physics and Applications
- Spectroscopy and Chemometric Analyses
- Proteoglycans and glycosaminoglycans research
- Vitamin C and Antioxidants Research
- Blood properties and coagulation
- Retinoids in leukemia and cellular processes
- Spectroscopy Techniques in Biomedical and Chemical Research
- Molecular Biology Techniques and Applications
- Lipid metabolism and biosynthesis
- Metabolism and Genetic Disorders
- Essential Oils and Antimicrobial Activity
University of the Basque Country
2013-2024
University of Vienna
2006
Hospital Oncológico Docente "Conrado Benítez García"
2002
Six flavone mono-C-glucosides, four standards (beta-D-glucopyranosyl-(1 --> C-6)- and -(1 C-8)- apigenin luteolin) two others from lemon juice C-8)-diosmetin) have been studied in order to analyze their fragmentation patterns. Initial separation was carried out using high-performance liquid chromatography with diode-array detection (HPLC/DAD) coupled an electrospray ionization (ESI) interface a triple quadrupole mass spectrometer. Several systematic differences between collision-induced...
The polyphenolic compositions of 31 Basque cider apple cultivars were determined in pulp, peel, and juice by high-performance liquid chromatography with diode array detection analysis crude extracts after thiolysis. Total polyphenols are distributed a wide concentration range depending on the cultivar. Procyanidins class that present major concentrations apple. Their average degrees polymerization from 4 to 8 Apple technologically classified into bitter nonbitter categories using different...
The data set composed by phenolic compound profiles of 83 Citrus juices (determined HPLC-DAD-MS/MS) was evaluated chemometrics to differentiate them according species (sweet orange, tangerine, lemon, and grapefruit). Cluster analysis (CA) principal component (PCA) showed natural sample grouping among even the subclass. Most information contained in full can be captured if only 15 compounds (concentration ≥10 mg/L), which quantified with fast accurate methods real samples, are introduced...
A mass spectrometric method using electrospray ionization with triple quadrupole and time-of-flight hybrid (Q-Tof) spectrometry has been applied to the structural characterization of dihydroflavonols. This family compounds studied by liquid chromatography/tandem (LC/MS/MS) for first time in this work. comprehensive study product ion MS spectra [M+H](+) a commercially available standard performed. The most useful fragmentations terms identification are those that involve cleavage C-ring,...
Lipid imaging mass spectrometry (LIMS) has been tested in several pathological contexts, demonstrating its ability to segregate and isolate lipid signatures complex tissues, thanks the technique's spatial resolution. However, it cannot yet compete with superior identification power of high-performance liquid chromatography coupled (HPLC-MS), therefore, very often, latter is used refine assignment species detected by LIMS. Also, not clear if differences sensitivity resolution between two...
Abstract Fifteen flavonoid O ‐diglycosides with different interglycosidic linkage isomery and glycosylation position have been studied in order to analyze their fragmentation patterns. Initial separation was carried out using high performance liquid chromatography diode array detection (HPLC/DAD) coupled an electrospray ionization (ESI) interface a triple quadrupole mass spectrometer. Some useful differences MS spectra found discussed. As it has already reported, [Y*] + /[Y 0 ] ratio for...
Abstract In hormone-responsive breast cancer cells, progesterone (P4) has been shown to act via its nuclear receptor (nPR), a ligand-activated transcription factor. A small fraction of is palmitoylated and anchored the cell membrane (mbPR) forming complex with estrogen alpha (ERα). Upon hormone exposure, either directly or interaction ERα, mbPR activates SRC/RAS/ERK kinase pathway leading phosphorylation nPR by ERK. Kinase activation essential for P4 gene regulation, as ERK MSK1 kinases are...
Human tumor xenografts in immunodeficient mice are a very popular model to study the development of cancer and test new drug candidates. Among parameters analyzed variations lipid composition, as they good indicators changes cellular metabolism. Here, we present on distribution lipids NCI-H1975 human lung cells, using MALDI imaging mass spectrometry UHPLC-ESI-QTOF. The identification directly from tissue by was aided comparison with ESI ionization extracts same xenografts. Lipids belonging...
This work intends to describe the physical properties of red blood cell (RBC) membranes in obese adults. The hypothesis driving this research is that obesity, addition increasing amount body fat, will also modify lipid composition cells other than adipocytes. Forty-nine control volunteers (16 male, 33 female, BMI 21.8 ± 5.6 and 21.5 4.2 kg/m2, respectively) 52 subjects male 36 38.2± 11.0 40.7 8.7 were examined. two techniques applied atomic force microscopy (AFM) spectroscopy mode, which...
Background:Abnormal cholesterol metabolism changes the neuronal membrane and may promote amyloidogenesis. Oxysterols in cerebrospinal fluid (CSF) are related to Alzheimer’s disease (AD) biomarkers mild cognitive impairment dementia. Cholesterol turnover is important for axonal white matt er (WM) microstructure maintenance. Objective:We aim demonstrate that association of oxysterols, AD biomarkers, WM occurs early asymptomatic individuals. Methods:We studied inter-individual variability CSF...
Background and Aims Pyranoanthocyanins are a type of derivative formed by the cycloaddition reaction different compounds with anthocyanins during wine maturation ageing, show characteristic pyranic ring. This ring provides high stability to pigment, does not fragment in mass spectrometric experiments. Thus, aglycone ions these derivatives tandem spectrometry same fragmentation pattern as that corresponding precursor anthocyanin. The aim this work was identification pyranoanthocyanins present...
Melanoma is the deadliest form of skin cancer due to its ability colonize distant sites and initiate metastasis. Although these processes largely depend on lipid-based cell membrane scaffold, our understanding melanoma lipid phenotype lags behind most other aspects this tumor cell. Here, we examined a panel normal human epidermal nevus melanocytes primary metastatic lines determine whether distinctive cell-intrinsic lipidomes can discern non-neoplastic from neoplastic define their potential....