A5089392584- Metabolomics and Mass Spectrometry Studies
- Mass Spectrometry Techniques and Applications
- Cancer, Lipids, and Metabolism
- Immunotherapy and Immune Responses
- Neuropeptides and Animal Physiology
- Advanced Proteomics Techniques and Applications
- Inflammatory mediators and NSAID effects
- Lipid Membrane Structure and Behavior
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Eicosanoids and Hypertension Pharmacology
- Immune cells in cancer
- Neurological Disorders and Treatments
- Renin-Angiotensin System Studies
- Blood disorders and treatments
- Pancreatic function and diabetes
- Analytical Chemistry and Chromatography
- Cell Adhesion Molecules Research
- Extracellular vesicles in disease
- Spectroscopy Techniques in Biomedical and Chemical Research
- Ion-surface interactions and analysis
- Advanced Chemical Sensor Technologies
- Alcohol Consumption and Health Effects
- S100 Proteins and Annexins
- Stroke Rehabilitation and Recovery
- Diet, Metabolism, and Disease
Health Research Institute of the Balearic Islands
2015-2021
Hospital Universitario Son Espases
2014-2021
Research Institute of Health Sciences
2010-2013
Universitat de les Illes Balears
2010-2013
Academia Nacional de Medicina
1993-1999
Academy of Medicine
1999
Abstract In the current work, we evaluated effect of extracellular acidification on neutrophil physiology. Neutrophils suspended in bicarbonate-buffered RPMI 1640 medium adjusted to acidic pH values (pH 6.5–7.0) underwent: 1) a rapid transient increase intracellular free calcium concentration levels; 2) an forward light scattering properties; and 3) up-regulation surface expression CD18. By contrast, acidosis was unable induce neither production H2O2 nor release myeloperoxidase. Acidic also...
Background Nonsteroidal anti-inflammatory drugs (NSAIDs) are a family of COX1 and COX2 inhibitors used to reduce the synthesis pro-inflammatory mediators. In addition, inflammation often leads harmful generation nitric oxide. Efforts being done in discovering safer NSAIDs molecules capable inhibiting lipid mediators oxide side effects associated with long term therapies. Methodology/Principal Findings The analogue arachidonic acid (AA), 2-hydroxy-arachidonic (2OAA), was designed inhibit...
Xenografts are commonly used to test the effect of new drugs on human cancer. However, because their heterogeneity, analysis results is often controversial. Part problem originates in existence tumor cells at different metabolic stages: from metastatic necrotic cells, as it happens real tumors. Imaging mass spectrometry an excellent solution for yields detailed information not only composition tissue but also distribution biomolecules within tissue. Here, we use imaging determine...
Colorectal cancer (CRC) is the fourth leading cause of death in world. Despite screening programs, its incidence population below 50s increasing. Therefore, new stratification protocols based on multiparametric approaches are highly needed. In this scenario, lipidome emerging as a powerful tool to classify tumors, including CRC, wherein it has proven be sensitive cell malignization. Hence, possibility describe at level lipid species renewed interest investigate role specific pathologic...
In the absence of appropriate stimuli, polymorphonuclear neutrophils rapidly undergo characteristic changes indicative programmed cell death or apoptosis. We report here that cultured in presence platelets (neutrophil:platelet ratios 1:50, 1:25, and 1:10) show a dramatic inhibition apoptosis compared with alone. Similar degrees delay were induced by viable unstimulated platelets, fixed activated (1 U/ml thrombin) platelets. Inhibition was associated prolongation functional lifespan...
Human tumor xenografts in immunodeficient mice are a very popular model to study the development of cancer and test new drug candidates. Among parameters analyzed variations lipid composition, as they good indicators changes cellular metabolism. Here, we present on distribution lipids NCI-H1975 human lung cells, using MALDI imaging mass spectrometry UHPLC-ESI-QTOF. The identification directly from tissue by was aided comparison with ESI ionization extracts same xenografts. Lipids belonging...
The mechanism of action 2-hydroxyoleic acid (2OHOA), a potent antitumor drug, involves the rapid and specific activation sphingomyelin synthase (SMS), leading to 4-fold increase in SM mass tumor cells. In present study, we investigated source ceramides required sustain this dramatic SM. Through radioactive fluorescent labeling, demonstrated that sphingolipid metabolism was altered by 24 h exposure 2OHOA, observed consistent number lysosomes presence unidentified storage materials treated...
Losartan, a selective antagonist of AT1 receptors for angiotensin II, is widely used clinically to manage hypertension. We report here that losartan markedly inhibits neutrophil shape change, adherence and chemiluminescence responses triggered by N-formylmethionyl-leucyl-phenylalanine (fMLP), without affecting induced immune complexes, zymosan or concanavalin A. Neither saralasin, another II receptors, nor captopril, an angiotensin-converting enzyme inhibitor, reproduced the effects...
Xenografts are a popular model for the study of action new antitumor drugs. However, xenografts highly heterogeneous structures, and therefore it is sometimes difficult to evaluate effects compounds on tumor metabolism. In this context, imaging mass spectrometry (IMS) may yield required information, due its inherent characteristics sensitivity spatial resolution. To best our knowledge, there still no clear analysis protocol properly changes between samples treatment. Here we present...
Summary The interaction of immunoglobulin G (IgG) antibodies with FcγR constitutes a critical mechanism through which IgG antibody effector functions are mediated. In the current work we have examined whether human neutrophil exhibit pH dependence in their association IgG. Binding assays were performed culture medium adjusted to different values. It was found that binding either heat‐aggregated (AIgG), soluble immune complexes (sIC) or IgG‐coated erythrocytes (IgG‐E) markedly higher at 6·5...
Even though colorectal cancer (CRC) is one of the most preventable cancers, it deadliest, and recent data show that incidence in people <50 years has unexpectedly increased. While new techniques for CRC molecular classification are emerging, no feature as yet firmly associated with prognosis. Imaging mass spectrometry (IMS) lipidomic analyses have demonstrated specificity lipid fingerprint differentiating pathological from healthy tissues. During IMS analysis, formation ionic adducts...
Abstract The epithelial layer of the human colon form finger-like invaginations into underlying connective tissue lamina propria to establish basic functional unit intestine, crypt. Adult stems cells, located at crypt base, proliferate and differentiate mature lineages surface epithelium. It is know that any alteration pathways regulating stem cell renewal leads tumor formation. In this context, little about how processes as differentiation or tumorigenesis affect one critical components a...