A5082144237- Mass Spectrometry Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Advanced Proteomics Techniques and Applications
- Advanced Chemical Sensor Technologies
- Molecular Biology Techniques and Applications
- Analytical Chemistry and Chromatography
- Spectroscopy Techniques in Biomedical and Chemical Research
- Nutrition, Genetics, and Disease
- Probiotics and Fermented Foods
- Identification and Quantification in Food
- Ion-surface interactions and analysis
- Digestive system and related health
- Adipose Tissue and Metabolism
- Cutaneous Melanoma Detection and Management
- Sulfur Compounds in Biology
- Diet, Metabolism, and Disease
- Social Policy and Reform Studies
- Psychological Treatments and Disorders
- Food Allergy and Anaphylaxis Research
- GNSS positioning and interference
- Infant Nutrition and Health
- Polyamine Metabolism and Applications
- Meat and Animal Product Quality
- Cancer, Lipids, and Metabolism
- Social Media and Politics
Biosensores (Spain)
2021-2024
University of the Basque Country
1988-2023
Se presenta aquí la introducción a Historia originaria de consciencia Erich Neumann, obra hasta ahora inédita en español. Basándose el desarrollo psicología Carl G. Jung, nuestro autor nos habla manera que inconsciente se manifiesta través imágenes primordiales o colectivas carácter mítico. Estas son las encargadas activar y su desarrollo. Al igual los rasgos morfológicos del cuerpo, según psicólogo, elementos estructurales psique heredados causa “infalible” desórdenes psicóticos si...
Xenografts are commonly used to test the effect of new drugs on human cancer. However, because their heterogeneity, analysis results is often controversial. Part problem originates in existence tumor cells at different metabolic stages: from metastatic necrotic cells, as it happens real tumors. Imaging mass spectrometry an excellent solution for yields detailed information not only composition tissue but also distribution biomolecules within tissue. Here, we use imaging determine...
Imaging mass spectrometry (IMS) is becoming an essential technique in lipidomics. Still, many questions remain open, precluding it from achieving its full potential. Among them, identification of species directly the tissue paramount importance. However, not easy task, due to abundance and variety lipid species, their numerous fragmentation pathways, formation a significant number adducts, both with matrix cations present tissue. Here, we explore pathways 17 classes, demonstrating that...
Sublimation is a widely used method for matrix deposition in imaging mass spectrometry experiments. Still, most of the time, standard glass sublimators are this purpose, which do not enable optimal reproducibility, compromising inter-experiment comparison results. Here, we present an in-house designed stainless steel sublimator parameters that have strongest influence over reproducibility can be easily monitored. Using sections human colon biopsies, demonstrate capabilities new prototype.
The recent developments in mass spectrometry have revealed the importance of lipids as biomarkers context different diseases and indicators cell's homeostasis. However, further advances are required to unveil complex relationships between lipid classes species with proteins. Here, we present a new methodology that combines microarrays obtain fingerprint samples nature standardized fast way, minimal sample consumption. As proof concept, use 20 rat tissues create library for tissue...
Knowledge on the normal structure and molecular composition of peripheral nerves is essential to understand their pathophysiology select regeneration strategies after injury. However, precise lipid nerve still poorly known. Here, we present first study distribution individual lipids in mature sciatic rats by imaging mass spectrometry. Both positive negative ion modes were used detect, identify situ map 166 species mainly glycerophospholipids, sphingomyelins, sulfatides, diacyl...
Abstract Ajzen and Fishbein 's attitudes model was applied to the prediction of voting in Autonomous Basque Parliament Elections held on 30th November 1986. On whole, model's predictive power acceptable. Nevertheless, found improve considerably when past experience taken into account.
In December 2021, the BIOFISH 300 SUL method for determination of total sulfites in shrimp was adopted as a First Action Official Method AnalysisSM by AOAC INTERNATIONAL.
Imaging mass spectrometry (IMS) is quickly becoming a technique of reference to visualize the lipid distribution in tissue sections. Still, many questions remain open, and data analysis has be optimized avoid interpretation pitfalls. Here we analyze how variation on [Na+]/[K+] relative abundance affects detection lipids between sections spinal cord (uninjured) control rats models demyelination traumatic contusion injury. The [M + Na]+/[M K]+ adducts ratio remained approximately constant...
Xenografts are a popular model for the study of action new antitumor drugs. However, xenografts highly heterogeneous structures, and therefore it is sometimes difficult to evaluate effects compounds on tumor metabolism. In this context, imaging mass spectrometry (IMS) may yield required information, due its inherent characteristics sensitivity spatial resolution. To best our knowledge, there still no clear analysis protocol properly changes between samples treatment. Here we present...
Even though colorectal cancer (CRC) is one of the most preventable cancers, it deadliest, and recent data show that incidence in people <50 years has unexpectedly increased. While new techniques for CRC molecular classification are emerging, no feature as yet firmly associated with prognosis. Imaging mass spectrometry (IMS) lipidomic analyses have demonstrated specificity lipid fingerprint differentiating pathological from healthy tissues. During IMS analysis, formation ionic adducts...
Probably, the most important factor for survival of a melanoma patient is early detection and precise diagnosis. Although in cases these tasks are readily carried out by pathologists dermatologists, there still difficult which no consensus among experts achieved. To deal with such cases, new methodologies required. Following this motivation, we explore here use lipid imaging mass spectrometry as complementary tool aid Thus, 53 samples (15 nevus, 24 primary melanomas, 14 metastasis) were...
Cheeses are becoming a major safety and public health concern: cheeses available in supermarkets occasionally contain high histamine concentrations that can have negative effects on consumer health. In this study, we attempted to assess the distribution pattern ripened cheeses, with purpose of establishing correct cheese sampling strategy for quantification histamine. To aim, was determined four distinct areas twelve long-ripened hard cheeses: external internal rind, along outer inner core...
Here, we present a simple and cost-effective procedure to improve the spatial resolution of commercial MALDI source LTQ Orbitrap. Based in filtering techniques, demonstrate that, with minimal modifications original setup, system can be pushed forward <10 μm. The improved performance is demonstrated by means imaging human colon biopsies.
Abstract Background Sulfite is the oldest and most widely used additive in our food supply with antioxidant preservative properties. Due to its allergenic-like reactions other adverse health effects, use regulated by international regulatory bodies. Therefore, industries as well laboratories must ensure that maximum concentration of sulfite permitted not exceeded. The AOAC INTERNATIONAL-approved official method for quantification sulfites Optimized Monier–Williams Method (AOAC Official...
In 2018, the AOAC Stakeholder Panel on Strategic Food Analytical Methods approved Standard Method Performance Requirement (SMPR®) 2018.009, for lactose in low-lactose or lactose-free milk, milk products, and products containing dairy ingredients, establishing minimum recommended performance characteristics to be addressed during evaluation of methods. Subsequently, INTERNATIONAL opened a call methods under Official AnalysisSM program with aim finding candidate method confirming compliance...
In April 2020, the BIOMILK 300 LAC method for quantification of lactose in lactose-free and low-lactose dairy products was adopted as First Action Official Method AnalysisSM2020.09 by AOAC INTERNATIONAL. To test reproducibility method, last step toward Final status, a collaborative study organized BIOLAN Microbiosensores.Fifteen collaborators within European Union took part this study, where nine different samples were sent duplicate blind portions determination. The data obtained used to...