A5020611952- Mass Spectrometry Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Advanced Proteomics Techniques and Applications
- Neuroscience and Neuropharmacology Research
- Molecular Biology Techniques and Applications
- Analytical Chemistry and Chromatography
- Cannabis and Cannabinoid Research
- Systemic Lupus Erythematosus Research
- Ion-surface interactions and analysis
- Pancreatic function and diabetes
- Cancer, Lipids, and Metabolism
- Cutaneous Melanoma Detection and Management
- Adipose Tissue and Metabolism
- Cellular transport and secretion
- SARS-CoV-2 and COVID-19 Research
- Aging, Health, and Disability
- Medicinal Plant Research
- Glycogen Storage Diseases and Myoclonus
- Diet, Metabolism, and Disease
- Vehicle emissions and performance
- Advanced Biosensing Techniques and Applications
- Coffee research and impacts
- Milk Quality and Mastitis in Dairy Cows
- Autonomous Vehicle Technology and Safety
- Sulfur Compounds in Biology
University of the Basque Country
2011-2023
Marqués de Valdecilla University Hospital
2022
Cystathionine beta-synthase (CBS) is a key regulator of sulfur amino acid metabolism diverting homocysteine, toxic intermediate the methionine cycle, via transsulfuration pathway to biosynthesis cysteine. Although itself well conserved among eukaryotes, properties eukaryotic CBS enzymes vary greatly. Here we present side-by-side biochemical and biophysical comparison human (hCBS), fruit fly (dCBS) yeast (yCBS) enzymes. Preparation characterization full-length truncated enzymes, lacking...
Xenografts are commonly used to test the effect of new drugs on human cancer. However, because their heterogeneity, analysis results is often controversial. Part problem originates in existence tumor cells at different metabolic stages: from metastatic necrotic cells, as it happens real tumors. Imaging mass spectrometry an excellent solution for yields detailed information not only composition tissue but also distribution biomolecules within tissue. Here, we use imaging determine...
Imaging mass spectrometry (IMS) is becoming an essential technique in lipidomics. Still, many questions remain open, precluding it from achieving its full potential. Among them, identification of species directly the tissue paramount importance. However, not easy task, due to abundance and variety lipid species, their numerous fragmentation pathways, formation a significant number adducts, both with matrix cations present tissue. Here, we explore pathways 17 classes, demonstrating that...
The detection of Scedosporium/Lomentospora is still based on non-standardized low-sensitivity culture procedures. This fact particularly worrying in patients with cystic fibrosis (CF), where these fungi are the second most common filamentous isolated, because a poor and delayed diagnosis can worsen prognosis disease. To contribute to discovery new diagnostic strategies, rapid serological dot immunobinding assay (DIA) that allows serum IgG against less than 15 min was developed. A crude...
Sublimation is a widely used method for matrix deposition in imaging mass spectrometry experiments. Still, most of the time, standard glass sublimators are this purpose, which do not enable optimal reproducibility, compromising inter-experiment comparison results. Here, we present an in-house designed stainless steel sublimator parameters that have strongest influence over reproducibility can be easily monitored. Using sections human colon biopsies, demonstrate capabilities new prototype.
The recent developments in mass spectrometry have revealed the importance of lipids as biomarkers context different diseases and indicators cell's homeostasis. However, further advances are required to unveil complex relationships between lipid classes species with proteins. Here, we present a new methodology that combines microarrays obtain fingerprint samples nature standardized fast way, minimal sample consumption. As proof concept, use 20 rat tissues create library for tissue...
Human tumor xenografts in immunodeficient mice are a very popular model to study the development of cancer and test new drug candidates. Among parameters analyzed variations lipid composition, as they good indicators changes cellular metabolism. Here, we present on distribution lipids NCI-H1975 human lung cells, using MALDI imaging mass spectrometry UHPLC-ESI-QTOF. The identification directly from tissue by was aided comparison with ESI ionization extracts same xenografts. Lipids belonging...
Knowledge on the normal structure and molecular composition of peripheral nerves is essential to understand their pathophysiology select regeneration strategies after injury. However, precise lipid nerve still poorly known. Here, we present first study distribution individual lipids in mature sciatic rats by imaging mass spectrometry. Both positive negative ion modes were used detect, identify situ map 166 species mainly glycerophospholipids, sphingomyelins, sulfatides, diacyl...
In Charcot-Marie-Tooth type 1A (CMT1A), Schwann cells exhibit a preponderant transcriptional deficiency of genes involved in lipid biosynthesis. This perturbed metabolism affects the peripheral nerve physiology and structure myelin. Nevertheless, identification functional characterization species mainly responsible for CMT1A myelin impairment currently lack. is critical pathogenesis neuropathy since lipids are many complex molecules which play essential roles cell, including structural...
In order to better understand retinal physiology, alterations which underlie some ocular diseases, we set out establish the lipid signature of two fundamental cell types in retina, Müller Glia and Retinal Ganglion Cells (RGCs). Moreover, compared these cells sections (in situ), as well after culturing isolating their membranes vitro). The lipidome glia RGCs was analyzed porcine using Matrix Assisted Laser Desorption Ionization Imaging Mass Spectrometry (MALDI-IMS). Isolated membranes, whole...
Cannabis has been used for decades as a palliative therapy in the treatment of cancer. This is because its beneficial effects on pain and nausea that patients can experience result chemo/radiotherapy. Tetrahydrocannabinol cannabidiol are main compounds present sativa, both exert their actions through receptor-mediated mechanism non-receptor-mediated mechanism, which modulates formation reactive oxygen species. These oxidative stress conditions might trigger lipidic changes, would compromise...
Recent advancements in mass spectrometry have significantly enhanced our understanding of complex lipid profiles, opening new avenues for oncological diagnostics. This review highlights the importance lipidomics comprehension certain metabolic pathways and its potential detection characterization various cancers, particular melanoma. Through detailed case studies, we demonstrate how lipidomic analysis has led to significant breakthroughs identification cancer types detecting unique...
Imaging mass spectrometry (IMS) is quickly becoming a technique of reference to visualize the lipid distribution in tissue sections. Still, many questions remain open, and data analysis has be optimized avoid interpretation pitfalls. Here we analyze how variation on [Na+]/[K+] relative abundance affects detection lipids between sections spinal cord (uninjured) control rats models demyelination traumatic contusion injury. The [M + Na]+/[M K]+ adducts ratio remained approximately constant...
The development of Cannabis sativa strains with high cannabidiol (CBD) and low tetrahydrocannabinol (THC) content is a growing field research, both for medical recreational use. However, the mechanisms behind clinical actions cannabinoids are still under investigation, although there evidence that mitochondria play an important role in many them. Numerous studies have described modulate mitochondrial activity through activation cannabinoid receptors direct action on other proteins such as...
Immune disorders arise from complex genetic and environmental factors, which lead to dysregulation at the cellular inflammatory levels cause tissue damage. Recent research highlights crucial role of reactive antibodies in autoimmune diseases graft rejection, but their determination poses challenges for clinical use. Therefore, our study aimed ascertain whether presence against membrane antigens tissues both animal models humans could serve as biomarkers patients with disorders. To address...
Multiple evidences suggest that mitochondrial dysfunction is implicated in the pathogenesis of Parkinson's disease via selective cell death dopaminergic neurons, such as which occurs after prolonged exposure to electron transport chain (ETC) complex I inhibitor, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyrine (MPTP). However, effects chronic MPTP on ETC complexes and enzymes lipid metabolism have not yet been thoroughly determined. To face these questions, enzymatic activities lipidomic profile...
Xenografts are a popular model for the study of action new antitumor drugs. However, xenografts highly heterogeneous structures, and therefore it is sometimes difficult to evaluate effects compounds on tumor metabolism. In this context, imaging mass spectrometry (IMS) may yield required information, due its inherent characteristics sensitivity spatial resolution. To best our knowledge, there still no clear analysis protocol properly changes between samples treatment. Here we present...