A5042101894- HIV Research and Treatment
- Immune Cell Function and Interaction
- HIV-related health complications and treatments
- Immune Response and Inflammation
- Chemokine receptors and signaling
- Immunotherapy and Immune Responses
- HIV/AIDS Research and Interventions
- interferon and immune responses
- Hepatitis C virus research
- HIV/AIDS drug development and treatment
- Cytomegalovirus and herpesvirus research
- Milk Quality and Mastitis in Dairy Cows
- T-cell and B-cell Immunology
- Inflammasome and immune disorders
- Adrenal Hormones and Disorders
- COVID-19 Clinical Research Studies
- Cell Adhesion Molecules Research
- Infections and bacterial resistance
- Immune cells in cancer
- Cytokine Signaling Pathways and Interactions
- Liver physiology and pathology
- Erythrocyte Function and Pathophysiology
- Mycobacterium research and diagnosis
- Chromium effects and bioremediation
- Transgenic Plants and Applications
Istituto Superiore di Sanità
2009-2024
Center for Global Health
2024
Vita-Salute San Raffaele University
2003
National Cancer Institute
1999
Sapienza University of Rome
1995-1997
National University of Luján
1992-1993
Università Cattolica del Sacro Cuore
1991
Abstract We previously reported that resting mouse peritoneal macrophages (PM) constitutively express low levels of IFN-gamma, whose production is consistently enhanced by exogenous IFN-gamma. In this study, we investigated the effects IL-12 on replication vesicular stomatitis virus and IFN-gamma gene expression in PM. The addition to freshly explanted PM resulted persistence an antiviral state virus, while control progressively became permissive for after 3 4 days culture. IL-12-induced was...
<h3>Objective</h3> In patients with hepatitis C virus (HCV)/HIV co-infection, a faster progression of liver fibrosis to cirrhosis has been reported. this study, an investigation was carried out determine whether gp120, HIV envelope protein, modulates the biology human hepatic stellate cells (HSCs), key cell types in pathogenesis fibrosis. <h3>Methods</h3> Myofibroblastic HSCs were isolated from normal tissue. Gene expression measured by real-time PCR. Cell migration assessed Boyden chambers....
ABSTRACT Dendritic cells (DCs) play a crucial role in bridging innate and acquired immune responses to pathogens. In human immunodeficiency virus type 1 (HIV-1) infection, immature DCs (iDCs) are also main targets for HIV-1 at the mucosal level. this study, we evaluated effects of HIV-1-DC interactions on maturation functional activity these cells. Exposure monocyte-derived iDCs either aldrithiol-2-inactivated or gp120 led an upmodulation activation markers indicative maturation. Despite...
In this study, we evaluated the effects of human immunodeficiency virus type 1 (HIV-1) and its gp120 protein on interleukin-10 (IL-10) expression in cultured monocytes/macrophages. Infection either 1-day monocytes or 7-day monocyte-derived macrophages with HIV-1 strain Ba-L resulted clear-cut accumulation IL-10 mRNA at 4 24 h. Likewise, treatment these cells recombinant induced caused a marked increase secretion. Monoclonal antibodies to strongly inhibited gp120-induced secretion by...
Abstract The present study was designed to evaluate the effect of HIV-1 envelope glycoprotein gp120 on expression β-chemokines in cultured monocytes/macrophages. Treatment either freshly isolated 1-day-cultured monocytes or 7-day-cultured monocyte-derived macrophages (MDM) with recombinant gp120-IIIB resulted a specific and dose-dependent enhancement secretion monocyte chemoattractant protein-1, macrophage inflammatory protein-1β, RANTES as well clear-cut increase transcript accumulation....
Macrophages are key targets of HIV-1 infection. We have previously described that the expression CC chemokine ligand 2 (CCL2) increases during monocyte differentiation to macrophages and it is further up-modulated by exposure. Moreover, CCL2 acts as an autocrine factor promotes viral replication in infected macrophages. In this study, we dissected molecular mechanisms which neutralization inhibits monocyte-derived (MDM), potential involvement innate restriction factors protein sterile alpha...
In vitro cultivated human monocytes show a time-dependent differentiation into macrophages, characterized by an increased expression of macrophage-specific antigens. Monocytes-macrophages were infected with immunodeficiency virus type 1 strain Ba-L (HIV-1Ba-L) at different stages differentiation. When 7-day cultured macrophages in the presence antibodies to beta interferon (IFN-beta), significant increase HIV-1 p24 release was detected. This effect not observed 1-day monocytes. finding...
The levels of genotypic relatedness among seven strains Leuconostoc spp. isolated from Argentine raw milk, lactis DSM 20202T (T = type strain) and 20198, mesenteroides subsp. NCDO 523T, dextranicum 529T, paramesenteroides 20288T were determined by performing a numerical analysis total soluble cell protein patterns DNA-DNA hybridization data. milk formed tight cluster at an r value 0.85 exhibited low DNA homology with the other species included in this study. These represent new within group...
SAMHD1 is a host restriction factor that functions to restrict both retroviruses and DNA viruses, based on its nuclear deoxynucleotide triphosphate (dNTP) hydrolase activity limits availability of intracellular dNTP pools. In the present study, we demonstrate expression was increased following human cytomegalovirus (HCMV) infection, with only modest effect infectious virus production. rapidly phosphorylated at residue T592 after infection by cellular cyclin-dependent kinases, especially...
Recombinant gp120, but not other human immunodeficiency type 1 (HIV-1) structural proteins, dose-dependently stimulates cytomegalovirus (HCMV) immediate-early antigen (IEA) expression and infectious virus yield in freshly isolated normal monocytes infected with HCMV. Monoclonal antibodies (MAbs) recognizing the gp120 V3 loop, as well loop octameric multibranched peptides antibody to galactocerebroside, sCD4, abrogate stimulation of IEA expression, suggesting that effect involves...
Apolipoprotein B mRNA editing enzyme catalytic polypeptide-like 3 (APOBEC3) family members are cytidine deaminases that play crucial roles in innate responses to retrovirus infection. The mechanisms by which some of these enzymes restrict HIV-1 replication have been extensively investigated vitro. However, little is known regarding how APOBEC3 proteins affect the pathogenesis infection vivo and antiretroviral therapy influences their expression. In this work, a longitudinal analysis was...
Abstract We characterized the IL-12 response of mouse macrophages in terms modulation IFN-γ production by cytokines (IFN-α and IL-18) regulation receptor expression. β1 β2 IL-12R chain mRNA expression increased with time culture absence exogenous stimulation, concomitant acquisition responsiveness to for production. Expression was further following treatment as a consequence regulated differentially IFN-α IL-18. Neutralization endogenous type I IFN secretion, whereas reduced it. In contrast,...
The monocyte/macrophage lineage represents heterogeneous cell populations characterized by major differences in the phenotype and functional activities. These cells are a source of soluble factors, such as cytokines chemokines, which can both affect HIV replication AIDS pathogenesis. Although monocytes/macrophages unanimously considered important targets HIV-1 infection, HIV-induced alterations their physiological functions at different stages differentiation still matter debate. In this...
HIV-1 envelope glycoprotein gp120 induces, independently of infection, the release CCL2 from macrophages. In turn, this chemokine acts as an autocrine factor enhancing viral replication. study, we show for first time that phosphoinositide-specific phospholipase C (PI-PLC) is required production triggered by in Using a combination confocal laser-scanner microscopy, pharmacologic inhibition, western blotting and fluorescence-activated cell sorter analysis, demonstrate interaction with CCR5...