A5005257877- Liver physiology and pathology
- Liver Disease Diagnosis and Treatment
- Trace Elements in Health
- Organ Transplantation Techniques and Outcomes
- Heavy Metal Exposure and Toxicity
- Liver Disease and Transplantation
- Pediatric Hepatobiliary Diseases and Treatments
- Drug Transport and Resistance Mechanisms
- TGF-β signaling in diseases
- Tissue Engineering and Regenerative Medicine
- Molecular Biology Techniques and Applications
- Pancreatic function and diabetes
- Pluripotent Stem Cells Research
- Photodynamic Therapy Research Studies
- Virus-based gene therapy research
- Veterinary Oncology Research
- Growth Hormone and Insulin-like Growth Factors
- Bone Metabolism and Diseases
- Veterinary Orthopedics and Neurology
- Hepatitis B Virus Studies
- Viral gastroenteritis research and epidemiology
- RNA Research and Splicing
- 3D Printing in Biomedical Research
- Fibroblast Growth Factor Research
- Spine and Intervertebral Disc Pathology
Utrecht University
2016-2025
Erasmus MC
2019
National Technical University of Athens
2019
University of Padua
2019
RWTH Aachen University
2010
California Health and Human Services Agency
2004
Leiden University
1988-2002
Vlaams Instituut voor Biotechnologie
1997-2000
Ghent University
1997
Ghent University Hospital
1995
Prospective observational and analytic study.To investigate whether spontaneous intervertebral disc degeneration (IVDD) occurring in both chondrodystrophic (CD) nonchondrodystrophic dogs (NCD) can be used as a valid translational model for human IVDD research.Different animal models are research, but most of these is induced manually or chemically rather than spontaneously.A total 184 discs (IVDs) from 19 different breeds were used. The extent was evaluated by macroscopic grading,...
Background and Aims The gap between patients on transplant waiting lists available donor organs is steadily increasing. Human organoids derived from leucine‐rich repeat‐containing G protein‐coupled receptor 5 (LGR5)–positive adult stem cells represent an exciting new cell source for liver regeneration; however, culturing large numbers of with current protocols tedious the level hepatic differentiation limited. Approach Results Here, we established a method expansion quantities human in...
Abstract End‐stage liver diseases are an increasing health burden, and transplantations currently the only curative treatment option. Due to a lack of donor livers, alternative treatments urgently needed. Human organoids very promising for regenerative medicine; however, cultured in Matrigel, which is extracted from extracellular matrix Engelbreth‐Holm‐Swarm mouse sarcoma. Matrigel poorly defined, suffers high batch‐to‐batch variability xenogeneic origin, limits clinical application...
The recent development of 3D-liver stem cell cultures (hepatic organoids) opens up new avenues for gene and/or therapy to treat liver disease. To test safety and efficacy, a relevant large animal model is essential but not yet established. Because its shared pathologies disease pathways, the dog considered best human Here we report establishment long-term canine hepatic organoid culture allowing undifferentiated expansion progenitor cells that can be differentiated toward functional...
Highlights•Feline organoids can be cultured from fresh and frozen liver needle biopsy•A feline-specific culture medium allows organoid expansion up to 32 passages•When fed fatty acids, feline store more lipids than human organoids•Feline used model hepatic steatosisSummaryHepatic steatosis is a highly prevalent disease, yet research hampered by the lack of tractable cellular animal models. Steatosis also occurs in cats, where it cause severe failure. Previous studies demonstrate potential...
The analysis of the inositol cycle in Dictyostelium discoideum cells is complicated by limited uptake [3H]inositol (0.2% applied radioactivity 6 h), and conversion into water-soluble metabolites that are eluted near position 1,4,5-trisphosphate [Ins(1,4,5)P3] on anion-exchange h.p.l.c. columns. was improved to 2.5% electroporation presence [3H]inositol; optimal at two 210 microseconds pulses 7 kV. Cells remained viable responsive chemotactic signals after electroporation. intracellular...
Early degeneration of the intervertebral disc (IVD) involves a change in cellular differentiation from notochordal cells (NCs) nucleus pulposus (NP) to chondrocyte-like (CLCs). The purpose this study was investigate gene expression profiles involved process using NP tissue non-chondrodystrophic and chondrodystrophic dogs, species with naturally occurring IVD degeneration.Dual channel DNA microarrays were used compare 1) healthy containing only NCs (NC-rich), 2) mixed population CLCs (Mixed),...
Abstract The notochordal cell (NC) of the nucleus pulposus (NP) is considered a potential NP progenitor cell, and early intervertebral disk (IVD) degeneration involves replacement NCs by chondrocyte‐like cells (CLCs). Wnt/β‐catenin signaling plays crucial role in maintaining fate during embryogenesis, but also involved tissue regeneration. canine species, which can be subdivided into non‐chondrodystrophic chondrodystrophic breeds, characterized differential maintenance NC: dogs, NC remains...
Background Transsphenoidal hypophysectomy is one of the treatment strategies in comprehensive management dogs with pituitary‐dependent hypercortisolism ( PDH ). Objectives To describe influence pituitary size at time gland surgery on long‐term outcome. Animals Three‐hundred–and‐six . Methods Survival and disease‐free fractions were analyzed related to size; without recurrence compared. Results Four weeks after surgery, 91% alive remission was confirmed 92% these dogs. The median survival 781...
The shortage of liver organ donors is increasing and the need for viable alternatives urgent. Liver cell (hepatocyte) transplantation may be a less invasive treatment compared with transplantation. Unfortunately, hepatocytes cannot expanded in vitro, allogenic requires long-term immunosuppression. Organoid-derived adult stem cells can cultured indefinitely to create sufficient numbers transplantation, they are amenable gene correction. This study provides preclinical proof concept potential...
Biochemical indicators for diagnosing liver disease are plasma alanine aminotransferase activity (ALT), alkaline phosphatase (ALP), and bile acid concentration (BA).To determine the sensitivity specificity of ALT, ALP, BA detecting primary hepatitis (PH) in clinically healthy Labrador retrievers investigate whether ALT ALP can discriminate between dogs with PH nonspecific reactive (RH).191 51 ill hepatic histopathology.Retrospective study. Medical records were reviewed preprandial BA,...