A5071868509- Glycosylation and Glycoproteins Research
- Lipid Membrane Structure and Behavior
- Monoclonal and Polyclonal Antibodies Research
- Bacteriophages and microbial interactions
- Escherichia coli research studies
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Galectins and Cancer Biology
- Toxin Mechanisms and Immunotoxins
- Transgenic Plants and Applications
- Carbohydrate Chemistry and Synthesis
- Insect Resistance and Genetics
- Insect symbiosis and bacterial influences
- Bryophyte Studies and Records
- Cellular transport and secretion
- Vibrio bacteria research studies
- Protist diversity and phylogeny
- Cellular Mechanics and Interactions
- Plant nutrient uptake and metabolism
- Salmonella and Campylobacter epidemiology
- Studies on Chitinases and Chitosanases
- Erythrocyte Function and Pathophysiology
- Botany and Plant Ecology Studies
- Legume Nitrogen Fixing Symbiosis
- Entomopathogenic Microorganisms in Pest Control
University of Freiburg
2020-2023
University of Copenhagen
2017
Vilnius University
2013
Significance The large superfamily of cadherins serve essential roles in cell–cell interactions and guidance. extracellular cadherin (EC) domains responsible for the biological functions are decorated with O-linked mannose glycans, but these O-glycans poorly understood. Here we describe an O-mannosylation pathway orchestrated by four homologous TMTC1–4 genes that is dedicated selectively to superfamily. Mutations TMTC3 gene cause cobblestone lissencephaly, demonstrating importance this type...
In plants, the topological organization of membranes has mainly been attributed to cell wall and cytoskeleton. Additionally, few proteins, such as plant-specific remorins have shown function protein lipid organizers. Root nodule symbiosis requires continuous membrane re-arrangements, with bacteria being finally released from infection threads into membrane-confined symbiosomes. We found that mutations in symbiosis-specific SYMREM1 gene result highly disorganized perimicrobial membranes....
Many Gram-negative bacterial pathogens use a syringe-like apparatus called type III secretion system to inject virulence factors into host cells. Some of these effectors are enzymes that modify proteins subvert their normal functions. NleB is glycosyltransferase modifies with N-acetyl-d-glucosamine inhibit antibacterial and inflammatory responses. conserved among the attaching/effacing enterohemorrhagic Escherichia coli (EHEC), enteropathogenic E. (EPEC), Citrobacter rodentium. Moreover,...
Abstract The link between cancer and aberrant glycosylation has recently become evident. Glycans their altered forms, known as tumour-associated carbohydrate antigens (TACAs), are diverse, complex difficult to target therapeutically. Lectins naturally occurring glycan-binding proteins that offer a unique opportunity recognise TACAs. T cells expressing chimeric antigen receptors (CARs) have proven be successful immunotherapy against leukaemias, but so far shown limited success in solid...
A chimeric, bispecific Janus lectin has recently been engineered with different, rationally oriented recognition sites. It can bind simultaneously to sialylated and fucosylated glycoconjugates. Because of its multivalent architecture, this reaches nanomolar avidities for sialic acid fucose. The was designed detect hypersialylation—a dysregulation in physiological glycosylation patterns, which promotes the tumor growth progression several cancer types. In study, characteristic properties were...
Abstract Carminic acid, a glucosylated anthraquinone found in scale insects like Dactylopius coccus , has since ancient times been used as red colorant various applications. Here we show that membrane-bound C-glucosyltransferase, isolated from D . and designated DcUGT2, catalyzes the glucosylation of flavokermesic acid kermesic into their respective C-glucosides dcII carminic acid. DcUGT2 is predicted to be type I integral endoplasmic reticulum (ER) membrane protein, containing cleavable...
Abstract Choanoflagellates are primitive protozoa used as models for animal evolution. They express a large variety of multi-domain proteins contributing to adhesion and cell communication, thereby providing rich repertoire molecules biotechnology. Adhesion often involves adopting β-trefoil fold with carbohydrate-binding properties therefore classified lectins. Sequence database screening dedicated method resulted in TrefLec, 44714 candidate lectins across 4497 species. TrefLec was searched...
Photorhabdus luminescens Tc toxins are large tripartite ABC-type toxin complexes, composed of TcA, TcB and TcC proteins. widespread have shown a tropism for variety targets including insect, mammalian human cells. However, their receptors the specific mechanisms uptake into target cells remain unknown. Here, we show that TcA protein TcdA1 interacts with N-glycans, particularly Lewis X/Y antigens. This is confirmed using N-acetylglucosamine transferase I (Mgat1 gene product)-deficient Chinese...
Membrane fusion is essential for the transport of macromolecules and viruses across membranes. While glycan-binding proteins (lectins) often initiate cellular adhesion, subsequent events require additional protein machinery. No mechanism membrane arising from simply a binding to glycolipids has been described thus far. Herein, we report that biotinylated derived cholera toxin becomes fusogenic lectin upon cross-linking with streptavidin. This novel reengineered brings about hemifusion...
The healthy heart adapts continuously to a complex set of dynamically changing mechanical conditions. environment is altered by, and contributes to, multiple cardiac diseases. Mechanical stimuli are detected transduced by cellular mechano-sensors, including stretch-activated ion channels (SAC). precise role SAC in the unclear, part because there few SAC-specific pharmacological modulators. That said, most can be activated inducers membrane curvature. lectin LecA virulence factor Pseudomonas...
Abstract A link between cancer and aberrant glycosylation has recently become evident. Glycans their altered forms, known as tumour-associated carbohydrate antigens (TACAs), are diverse, complex difficult to target therapeutically. Lectins naturally occurring glycan-binding proteins offer a unique opportunity recognise TACAs. T cells expressing chimeric antigen receptors (CARs) have proven be successful immunotherapy against leukaemias, but so far shown limited success in solid tumours. We...
Synthetic biology is a rapidly growing field with applications in biotechnology and biomedicine. Through various approaches, remarkable achievements, such as cell tissue engineering, have been already accomplished. In synthetic glycobiology, the engineering of glycan binding proteins being exploited for producing tools precise topology specificity. We developed concept engineered chimeric lectins, i.e., Janus lectin, increased valency, additional The novel assembled fusion protein between...
Abstract Lectins are efficient multivalent glycan receptors, deciphering the glyco-code on cell surfaces. The β-trefoil fold, characterized by three lobe-shaped repeats, is adopted several classes of lectins, often associated with other domains having enzymatic or toxic activity. Based UniLectin3D database classification, sequence signature trefoil lobes was defined and used to predict 44714 lectins from 4497 species. Among them, SaroL-1 lower eukaryote Salpingoeca rosetta predicted contain...
Abstract Synthetic biology is a rapidly growing field with applications in biotechnology and biomedicine. Through various approaches, remarkable achievements, such as cell tissue engineering, have been already accomplished. In synthetic glycobiology, the engineering of glycan binding proteins being developed for producing tools precise topology specificity. We concept chimeric lectins, i.e., Janus lectin, increased valency, additional The novel engineered assembled fusion protein between...
Membrane fusion is essential for the transport of macromolecules and viruses across membranes. While glycan-binding proteins (lectins) often initiate cellular adhesion, subsequent events require additional protein machinery. No mechanism membrane arising from simply a binding to glycolipids has been described thus far. Herein we report that biotinylated derived cholera toxin, becomes fusogenic lectin upon crosslinking with streptavidin. This novel reengineered brings about hemifusion...
The present report assesses the capability of a soluble glycosyltransferase to modify glycolipids organized in two synthetic membrane systems that are attractive models mimic cell membranes: giant unilamellar vesicles (GUVs) and supported lipid bilayers (SLBs). objective was synthesize Gb3 antigen (Galα1,4Galβ1,4Glcβ-Cer), cancer biomarker, at surface these models. A form LgtC adds galactose residue from UDP-Gal lactose-containing acceptors selected. Although less efficient than with...
Abstract Šiukštaitė L., Patamsytė J., Žvingila D., 2013: Impact of genetic and demographic factors on the expansion potential alien species Bunias orientalis in suburbs Vilnius [Genetinių ir demografinių veiksnių įtaka svetimkraštės rūšies vienos populiacijos plitimo galimybėms]. - Both. Lith., 19(2): 111-119. L. (Brassicaceae) is an already naturalized plant Lithuania. Our purpose was to assess diversity age structure within single Belmontas population B. orientalis, using RAPD, ISSR assays...
Abstract Funding Acknowledgements Type of funding sources: Public grant(s) – EU funding. Main source(s): This research was supported by the European Research Council (Advanced Grant CardioNECT, Project ID: #323099, PK) and a grant from Ministry Science, Arts Baden-Württemberg (MWK-BW Sonderlinie Medizin, #3091311631). Mechanical stimuli are detected transduced cellular mechano-sensors, including stretch-activated ion channels (SAC). SAC activated stretch changes in membrane curvature but...
Membrane fusion is essential for the transport of macromolecules and viruses across membranes. While glycan-binding proteins (lectins) often initiate cellular adhesion, subsequent events require additional protein machinery. No mechanism membrane arising from simply a binding to glycolipids has been described thus far. Herein we report that biotinylated derived cholera toxin, becomes fusogenic lectin upon crosslinking with streptavidin. This novel reengineered brings about hemifusion...