A5074590917- Antifungal resistance and susceptibility
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Transplantation: Methods and Outcomes
- Angiogenesis and VEGF in Cancer
- Cell Adhesion Molecules Research
- Biopolymer Synthesis and Applications
- Peptidase Inhibition and Analysis
- Cystic Fibrosis Research Advances
- Mycotoxins in Agriculture and Food
- Bioactive Compounds and Antitumor Agents
- Otitis Media and Relapsing Polychondritis
- Carbon Nanotubes in Composites
- Monoclonal and Polyclonal Antibodies Research
- Fungal Infections and Studies
- Telemedicine and Telehealth Implementation
- Galectins and Cancer Biology
- Biosensors and Analytical Detection
- Platelet Disorders and Treatments
- Hematopoietic Stem Cell Transplantation
- Viral Infectious Diseases and Gene Expression in Insects
- Social Media in Health Education
- Molecular Biology Techniques and Applications
- Nematode management and characterization studies
- Communication and COVID-19 Impact
- Advanced Drug Delivery Systems
Albany Medical Center Hospital
2024-2025
Stanford University
2018-2024
Pulmonary and Allergy Associates
2023
University of Pennsylvania
2014-2022
Cancer Research Center
2014-2022
Democritus University of Thrace
2014-2022
Abstract: Conventional chemotherapy of ovarian cancer often fails because initiation drug resistance and/or side effects and trace untouched remaining cancerous cells. This highlights an urgent need for advanced targeted therapies effective remediation the disease using a cytotoxic agent with immunomodulatory effects, such as shikonin (SHK). Based on preliminary experiments, we found SHK to be profoundly toxic in epithelial cells (OVCAR-5 ID8 cells) well normal IOSE-398 cells, endothelial...
Tumor endothelial marker 1 (TEM1; also known as endosialin or CD248) is a protein found on tumor vasculature and in stroma. Here, we tested whether TEM1 has potential therapeutic target for cancer immunotherapy by immunizing immunocompetent mice with Tem1 cDNA fused to the minimal domain of C fragment tetanus toxoid (referred herein Tem1-TT vaccine). vaccination elicited CD8+ and/or CD4+ T cell responses against immunodominant sequences. Prophylactic immunization animals prevented delayed...
Immune suppression heightens the risk for fungal infections, but mechanisms that result in clinical disease are poorly understood. Here we demonstrate macrophage ferroptosis, an iron-dependent form of regulated cell death, inhibits Aspergillus fumigatus (Af) killing. In a mouse tracheal transplant model Af infection, observed increase lipid peroxidation, decreased expression negative ferroptosis regulators Gpx4 and Slc7a11, positive Ptgs2 Nox2, relative to syntransplants. Depletion...
Introduction: Tumor endothelial marker 1 (TEM1) is expressed by tumor vascular cells in various cancers. Methods: Here, we developed poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) PEGylated with polyethylene glycol (PEG) and functionalized anti-TEM1 antibody fragment (78Fc) loaded them necroptosis-inducing agent shikonin (SHK) (78Fc-PLGA-SHK NPs). Results: The nanoformulation showed a smooth spherical shape (~120 nm; the ζ potential of -30 mV) high drug entrapment bioconjugation...
BACKGROUND. Invasive aspergillosis (IA) is a deadly fungal lung infection. Antifungal resistance and treatment side effects are major concerns. Iron chelators vital for IA management, but systemic use can cause effects. We developed nanoparticles (NPs) to selectively deliver the iron chelator deferasirox (DFX) treatment. METHODS DFX was encapsulated in poly(lacticco‐ glycolic acid) (PLGA) NPs using single emulsion solvent evaporation method. were characterized by light scattering electron...
Invasive aspergillosis (IA) is a deadly fungal lung infection. Antifungal resistance and treatment side effects are major concerns. Iron chelators vital for IA management, but systemic use can cause effects. We developed nanoparticles (NPs) to selectively deliver the iron chelator deferasirox (DFX) treatment.
Aspergillus mold is a ubiquitously found, airborne pathogen that can cause variety of diseases from mild to life-threatening in severity. Limitations diagnostic methods combined with anti-fungal resistance render global emerging pathogen. In industry, Aspergilli produce toxins, such as aflatoxins, which food spoilage and pose public health risk issues. Here, we report multiplex qPCR method for the detection identification five most common pathogenic species, fumigatus, flavus, niger,...
Cystic fibrosis (CF) lung transplant recipients (LTRs) exhibit a disproportionately high rate of life-threatening invasive aspergillosis (IA). Loss the cystic transmembrane conductance regulator (CFTR-/-) in macrophages (mφs) has been associated with lyosomal alkalinization. We hypothesize that this alkalinization would persist iron-laden post-transplant microenvironment increasing risk IA. To investigate our hypothesis, we developed murine CF orthotopic tracheal (OTT) model. Iron levels...
Meeting abstracts Tumor Endothelial Marker 1 (TEM1 or CD248) has been identified as a microvascular marker of tumor angiogenesis in various human cancers. By comparing the vasculature ovarian cancer and normal ovary, we others [[1][1]] that TEM1 is overexpressed
Infectious diseases plagued humanity throughout history, the most recent Coronavirus Disease 2019 (COVID-19) pandemic had a severe impact in world and revealed vulnerability of modern society to airway infections.