A5101465936- Immunotherapy and Immune Responses
- Lipid metabolism and biosynthesis
- Mitochondrial Function and Pathology
- Milk Quality and Mastitis in Dairy Cows
- Cancer Immunotherapy and Biomarkers
- Peroxisome Proliferator-Activated Receptors
- Virus-based gene therapy research
- Cancer Research and Treatments
- Metabolomics and Mass Spectrometry Studies
- Extracellular vesicles in disease
- Adipose Tissue and Metabolism
- Cancer Genomics and Diagnostics
- Hepatitis B Virus Studies
- Phytochemistry and biological activity of medicinal plants
- ATP Synthase and ATPases Research
- Sarcoma Diagnosis and Treatment
- Immune Cell Function and Interaction
- S100 Proteins and Annexins
- Peptidase Inhibition and Analysis
- Protein Kinase Regulation and GTPase Signaling
- T-cell and B-cell Immunology
- RNA Interference and Gene Delivery
- Machine Learning in Bioinformatics
- Ferroptosis and cancer prognosis
- Cancer, Lipids, and Metabolism
Air Force Medical University
2014-2025
Yancheng Teachers University
2024
Soochow University
2024
First Affiliated Hospital of Soochow University
2024
China Agricultural University
2021-2024
Xi'an University of Technology
2024
Xijing Hospital
2012-2023
Hunan Provincial Center for Disease Control and Prevention
2020
Changchun University of Science and Technology
2019
Affiliated Hospital of Nantong University
2018
Recently, we have identified the dramatic depletion of cardiolipin (CL) in diabetic myocardium 6 weeks after streptozotocin (STZ) injection that was accompanied by increases triacylglycerol content and multiple changes polar lipid molecular species. However, state, predominant hallmarks cardiomyopathy were each present concomitantly, thus, it impossible to identify temporal course alterations myocardium. Using newly developed enhanced shotgun lipidomics approach, demonstrated loss abundant...
Cardiolipin is a prominent component of the mitochondrial inner membranes contributing to regulation multiple discrete functions. Here, we extend shotgun lipidomics identify and quantitate cardiolipin molecular species directly from lipid extracts biological samples. Three approaches for analyses were developed using either continuous ion-transmission instrument (i.e., triple-quadrupole type) with low or high mass resolution settings hybrid pulsed [i.e., quadrupole time-of-flight (QqTOF)...
Diabetic cardiomyopathy is characterized by excessive utilization of fatty acid substrate, diminished glucose transport, and mitochondrial dysfunction. However, the chemical mechanisms linking altered substrate to dysfunction are unknown. Herein, we use shotgun lipidomics multidimensional mass spectrometry identify dramatic decreases in critical inner membrane lipid, cardiolipin, diabetic murine myocardium (from 7.2 ± 0.3 nmol/mg protein control hearts 3.1 0.1 myocardium; p < 0.001, n = 7)....
Background Polyphenols, a group of complex naturally occurring compounds, are widely distributed throughout the plant kingdom and therefore readily consumed by humans. The relationship between their chemical structure intestinal absorption, transport, first-pass metabolism remains unresolved, however. Methods Here, we investigated absorption four polyphenol apigenin, resveratrol, emodin chrysophanol, using in vitro Caco-2 cell monolayer model system situ perfusion vivo pharmacokinetic...
Dendritic cells (DCs) release bioactive exosomes that play an important role in immune regulation. Because they express low levels of class I major histocompatibility complex (MHC) and co-stimulatory molecules, derived from donor immature DCs (imDex) prolong allograft survival by inhibiting T-cell activation. However, this effect is limited does not induce immunological tolerance when imDex are administered alone. Thus, we tested the combined treatment with low-dose rapamycin on inducing a...
Large-scale neuronal remodeling through apoptosis occurs shortly after birth in all known mammalian species. Apoptosis, large part, depends upon critical interactions between mitochondrial membranes and cytochrome c. Herein, we examined the hypothesis that large-scale reorganization of circuitry is accompanied by profound alterations cardiolipin (CL) content molecular species distribution. During embryonic development, over 100 CL were identified quantitated murine tissues. The profile was...
Interleukin-37 (IL-37), a newly identified member of the IL-1 family, has been known to play an immunosuppressive role in variety inflammatory disorders, but whether it participates regulation pathogenesis non-small cell lung cancer (NSCLC) not investigated. Real-time polymerase chain reaction (PCR), western blotting, and immunohistochemical staining were employed detect IL-37 expression NSCLC tissues corresponding adjacent tissues. The correlations between clinicopathological...
Allograft tolerance is the ultimate goal in field of transplantation immunology. Immature dendritic cells (imDCs) play an important role establishing but have limitations, including potential for maturation, short lifespan vivo and storage times vitro. However, exosomes (generally 30-100 nm) from imDCs (imDex) retain many source cell properties may overcome these limitations. In previous reports, imDex prolonged survival time heart or intestine allografts. long-term was not achieved unless...
Patients with ER/HER2-positive breast cancer have a poor prognosis and are less responsive to selective estrogen receptor modulators; this is presumably due the crosstalk between ER HER2. Fatty acid synthase (FASN) essential for survival maintenance of malignant phenotype cells. An intimate relationship exists FASN, We hypothesized that FASN may be downstream effector underlying ER/HER2 through PI3K/AKT/mTOR pathway in cancer. The present study implicated regulation expression cells...
Membrane‐associated calcium‐independent phospholipase A 2 γ (iPLA γ) contains four potential in‐frame methionine start sites (Mancuso, D.J. Jenkins, C.M. & Gross, R.W. (2000) J. Biol. Chem. 275 , 9937–9945), but the mechanisms regulating types, amount and subcellular localization of iPLA in cells are incompletely understood. We now: (a) demonstrate dramatic transcriptional repression mRNA synthesis encoding by a nucleotide sequence nested coding itself; (b) localize site to most 5′...
Previously, we identified calcium-independent phospholipase A2γ (iPLA2γ) with multiple translation initiation sites and dual mitochondrial peroxisomal localization motifs. To determine the role of iPLA2γ in integrating lipid energy metabolism, generated transgenic mice containing α-myosin heavy chain promoter (αMHC) placed proximally to human coding sequence that resulted cardiac myocyte-restricted expression (TGiPLA2γ). TGiPLA2γ possessed phenotypes including: 1) a dramatic ∼35% reduction...
Abstract This study evaluated the association between free fatty acid (FFA), ROS generation, mitochondrial dysfunction and bone mineral density (BMD) in type 2 diabetic patients investigated molecular mechanism. db/db high fat (HF)-fed mice were treated by Etomoxir, an inhibitor of CPT1, MitoQ PFT-α, P53. Bone metabolic factors assessed BMSCs isolated induced to osteogenic differentiation. FFA, lipid peroxidation mtDNA copy number correlated with BMD T2DM patients. PFT-α significantly...
We conducted a case–control study to investigate the role of three common IL-17A and IL-17F single nucleotide polymorphisms (SNPs) on susceptibility gastric cancer. A was using Chinese population 462 cancer subjects controls. Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) taken genotype rs2275913, rs763780, rs3748067 within IL-17 gene. When comparing demographic characteristics between cases control groups, were more likely be cigarette smokers alcohol...
Herein, we exploit the power of global lipidomics to identify critical role peroxisomal processing fatty acids in adipocyte lipid storage and metabolism. Remarkably, 3T3-L1 differentiating adipocytes rapidly acquired ability α oxidize unbranched acids, which is manifested accumulation odd chain length all major classes. Moreover, adipocytes, unsaturated TAG molecular species contained exclusively Δ9 olefinic linkages. Unsaturated (e.g., oleic palmitoleic acids) were not subject oxidation,...
Streptococcus lutetiensis, an emerging pathogen causing bovine mastitis, has not been well characterized. We reported that S. lutetiensis was pathogenic both in vivo and vitro caused inflammatory reactions the mammary gland. However, roles of autophagy oxidative stress pathogenesis lutetiensis-induced mastitis are unclear. In this study, model lutetiensis-infected epithelial cells (bMECs) used to assess flux. Expressions Beclin1, light chain 3II, Sequestosome 1/p62 were elevated bMECs after...
With large-scale grid-connected renewable energy, new power systems require more flexible and reliable energy storage sources. Pumped stations play an important role in peak shaving, valley filling, promoting consumption. This paper presents the reasonable energy-abandonment operation of a combined generation system (CPGS), which pumped station is core control power, with ultra-high proportion energy. Firstly, based on seasonal characteristics wind, solar, load demand, typical days are...
Recently, a sequence encoding novel mammalian calcium‐independent phospholipase A 2 (iPLA γ) was identified in the human genome and subsequently cloned expressed Sf9 insect cells. Unexpectedly, expression studies recombinant systems demonstrated usage of multiple translation initiation codons resulting different polypeptides. Herein, we demonstrate that hepatic iPLA γ is localized to rat liver peroxisomes, possesses molecular mass 63 kDa peroxisomal membranes are highly enriched arachidonic...
The accumulation of fatty acids and their metabolites results in insulin resistance reduced glucose utilization through a variety complex mechanisms that remain incompletely understood. Herein, we demonstrate submicromolar concentrations palmitoyl-CoA inhibit glyceraldehyde-3-phosphate dehydrogenase (GAPDH; EC 1.2.1.12) enzyme activity the covalent thioesterification palmitate to GAPDH. First, incubation GAPDH with (0.5−5 μM) resulted dramatic concentration-dependent inhibition activity....