A5100367610- RNA Research and Splicing
- Pancreatic function and diabetes
- RNA and protein synthesis mechanisms
- Endoplasmic Reticulum Stress and Disease
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Cancer, Hypoxia, and Metabolism
- 3D Printing in Biomedical Research
- Cancer-related Molecular Pathways
- Cancer Cells and Metastasis
- Adipose Tissue and Metabolism
- Viral Infectious Diseases and Gene Expression in Insects
- Tissue Engineering and Regenerative Medicine
- Cancer Research and Treatments
- Virus-based gene therapy research
- PI3K/AKT/mTOR signaling in cancer
- Cellular Mechanics and Interactions
- Plant tissue culture and regeneration
- Advanced biosensing and bioanalysis techniques
- DNA Repair Mechanisms
- Cancer-related molecular mechanisms research
- Transgenic Plants and Applications
- Cell death mechanisms and regulation
- Pineapple and bromelain studies
- Advanced Biosensing Techniques and Applications
Washington State University Spokane
2015-2024
Moffitt Cancer Center
2016-2023
Scripps Research Institute
2012-2023
Chinese PLA General Hospital
2023
Sichuan University
2014-2022
West China Hospital of Sichuan University
2014-2022
Ministry of Agriculture and Rural Affairs
2021
Beijing University of Agriculture
2021
Georgia Highlands College
2015-2020
ZTE (China)
2020
Abstract Target occupancy is often insufficient to elicit biological activity, particularly for RNA, compounded by the longstanding challenges surrounding molecular recognition of RNA structures small molecules. Here we studied patterns between a natural-product-inspired small-molecule collection and three-dimensionally folded structures. Mapping these interaction landscapes across human transcriptome defined structure–activity relationships. Although RNA-binding compounds that bind...
Tissue matrix scaffold is an advanced tissue-mimicry 3D culture system for biomedical research and drug screening.
Dendritic cells (DCs) release bioactive exosomes that play an important role in immune regulation. Because they express low levels of class I major histocompatibility complex (MHC) and co-stimulatory molecules, derived from donor immature DCs (imDex) prolong allograft survival by inhibiting T-cell activation. However, this effect is limited does not induce immunological tolerance when imDex are administered alone. Thus, we tested the combined treatment with low-dose rapamycin on inducing a...
Background/Aims: Melatonin, synthesized by the pineal gland and released into blood, appears to have antitumour properties; however, mechanisms of its anti-cancer effects are largely unknown, especially in stomach cancer. Here, we explore activity melatonin a gastric cancer cell line (AGS) analyse molecular mechanisms. Methods: AGS cells were treated with melatonin, viability was assessed using CCK-8 assay. Flow cytometry performed evaluate apoptosis, protein expression examined Western...
A central regulator of cell growth that has been implicated in responses to stress such as hypoxia is mTOR ( m ammalian T arget O f R apamycin). We have shown previously required for angiogenesis vitro and endothelial proliferation response hypoxia. Here we investigated mTOR-associated signaling components under their effects on rat aortic cells (RAECs). Hypoxia (1% 2 ) rapidly (>30 minutes) a concentration-dependent manner promoted rapamycin-sensitive sustained phosphorylation...
Preparation of three-dimensional (3D) porous scaffolds from synthetic polymers is a challenge to most laboratories conducting biomedical research. Here, we present handy and cost-effective method fabricate polymeric hydrogel using poly(lactic-co-glycolic) acid (PLGA) or polycaprolactone (PCL). Breast cancer cells grown on 3D exhibited distinct survival, morphology, proliferation compared those 2D surfaces. Mammary epithelial cultured PLGA- PCL-coated slides expressed extracellular matrix...
Polyadenylation of nascent RNA by poly(A) polymerase (PAP) is important for 3′ end maturation almost all eukaryotic mRNAs. Most mammalian genes harbor multiple polyadenylation sites (PASs), leading to expression alternative (APA) isoforms with distinct functions. How polymerases may regulate PAS usage and hence gene poorly understood. Here, we show that the nuclear canonical (PAPα PAPγ) non-canonical (Star-PAP) PAPs play diverse roles in selection expression. Deficiencies resulted...
Cisplatin-induced nephrotoxicity limits its use in many cancer patients. The expression of enzymes involved polyamine catabolism, spermidine/spermine N1-acetyltransferase (SSAT) and spermine oxidase (SMOX) increase the kidneys mice treated with cisplatin. We hypothesized that enhanced catabolism contributes to tissue damage cisplatin acute kidney injury (AKI). Using gene knockout chemical inhibitors, role AKI was examined. Deficiency SSAT, SMOX or neutralization toxic products degradation,...
Melatonin, which is synthesized by the pineal gland and released into blood, exhibits antitumor properties. However, mechanisms underlying these effects, particularly in stomach cancer, remain unknown. In present study, effect of melatonin on nuclear factor (NF)-κB signaling pathway mitogen-activated protein kinase pathway, involving p38 c-Jun-N-terminal (JNK), were investigated SGC7901 gastric cancer cells. addition, survival, migration apoptosis cells was vitro order to evaluate use for...
Abstract Background Breast cancer cells invading the connective tissues outside mammary lobule or duct immerse in a reservoir of extracellular matrix (ECM) that is structurally and biochemically distinct from their site origin. The ECM spatial network proteins, which not only provide physical support but also serve as bioactive ligands to cells. It becomes evident dimensional, mechanical, structural, biochemical properties are all essential mediators many cellular functions. To better...
The G(1)/S-phase transition is a well-toned switch in the mammalian cell cycle. Cdk2, Cdk4, and rate-limiting tumor suppressor retinoblastoma protein (Rb) have been studied separate animal models, but interactions between kinases Rb vivo yet to be investigated. To further dissect regulation of G(1) S-phase progression, we generated Cdk2(-/-)Cdk4(-/-)Rb(-/-) (TKO) mutant mice. TKO mice died at midgestation with major defects circulatory systems displayed combined phenotypes Rb(-/-)...
Porcine mammary fatty tissues represent an abundant source of natural biomaterial for generation breast-specific extracellular matrix (ECM). Here we report the extraction total ECM proteins from pig breast tissues, fabrication hydrogel and porous scaffolds extracted proteins, structural properties (tissue scaffold, TMS), applications in human epithelial cell spatial cultures surface receptor expression, metabolomics characterization, acini formation, proliferation, migration between...
Meiotic recombination initiates following the formation of DNA double-strand breaks (DSBs) by Spo11 endonuclease early in prophase I, at discrete regions genome coined "hot spots." In mammals, meiotic DSB site selection is directed part sequence-specific binding PRDM9, a polymorphic histone H3 (H3K4Me3) methyltransferase. However, other chromatin features needed for hot spot specification are largely unknown. Here we show that recombinogenic cores active spots mice harbor several and H4...