A5037673121- Parasites and Host Interactions
- IL-33, ST2, and ILC Pathways
- Viral Infections and Immunology Research
- Research on Leishmaniasis Studies
- RNA regulation and disease
- Cytokine Signaling Pathways and Interactions
- Psoriasis: Treatment and Pathogenesis
- Cytomegalovirus and herpesvirus research
- Digestive system and related health
- Mosquito-borne diseases and control
- Viral Infections and Vectors
- NF-κB Signaling Pathways
- Health, psychology, and well-being
- Vitamin D Research Studies
- Whipple's Disease and Interleukins
- interferon and immune responses
- Malaria Research and Control
- RNA and protein synthesis mechanisms
- Cancer Cells and Metastasis
University of Massachusetts Chan Medical School
2018-2024
Tulane University
2008-2013
Tufts University
2007-2008
Abstract Helminth exposure appears to protect hosts from inappropriate inflammatory responses, such as those causing bowel disease. A recently identified, strongly proinflammatory limb of the immune response is characterized by T cell IL-17 production. Many autoimmune type diseases are associated with release. Because helminths these diseases, we examined production in helminth-colonized mice. We colonized mice Heligmosomoides polygyrus, an intestinal helminth, and analyzed lamina propria...
Abstract Introduction Despite intensive study of the mechanisms chemotherapeutic drug resistance in human breast cancer, few reports have systematically investigated that underlie to chemotherapy-sensitizing agent tumor necrosis factor (TNF)-α. Additionally, relationship between TNF-α mediated by MEK5/Erk5 signaling and epithelial-mesenchymal transition (EMT), a process associated with promotion invasion, metastasis, recurrence has not previously been investigated. Methods To compare...
Abstract In infection with the trematode helminth Schistosoma mansoni, severity of CD4 T cell-mediated hepatic granulomatous and fibrosing inflammation against parasite eggs varies considerably in humans among mouse strains. mice, either natural high pathology, or pathology induced by concomitant immunization schistosome egg Ags (SEA) CFA (SEA/CFA), results from a failure to contain net proinflammatory cytokine environment. We previously demonstrated that induction severe immunopathology was...
There are no available vaccines for dengue, the most important mosquito-transmitted viral disease. Mechanistic studies with anti-dengue virus (DENV) human monoclonal antibodies (hMAbs) provide a rational approach to identify and characterize neutralizing epitopes on DENV structural proteins that can serve inform vaccine strategies. Here, we report class of hMAbs is likely be an determinant in humoral response infection. In this study, identified characterized three broadly anti-DENV hMAbs:...
Abstract Infection with schistosomes results in a CD4 T cell-mediated inflammatory reaction against parasite eggs that varies greatly magnitude both humans as well mice. In the murine disease, severe form of immunopathology correlates high levels IL-17. We now report live schistosome stimulate dendritic cells from pathology-prone CBA mice to produce IL-12p40, IL-6, and TGF-β, whereas those low BL/6 only make TGF-β. Moreover, egg-stimulated plus naive resulted increased IL-23, IL-1β, IL-17...
ABSTRACT Infection with the trematode helminth Schistosoma mansoni results in a parasite egg-induced, CD4 T-cell-mediated, hepatointestinal granulomatous and fibrosing inflammation that varies greatly severity, higher frequency of milder forms typically occurring regions where disease is endemic. One possible explanation for this these degree lessened by widespread concurrent infection gastrointestinal nematodes. We tested hypothesis establishing murine coinfection model which mice were...
Viral myocarditis is a leading cause of death in the United States, contributing to numerous unexplained deaths people ≤35 years old. Enteroviruses contribute many cases human myocarditis. Encephalomyocarditis virus (EMCV) infection causes viral rodent models, but its receptor requirements have not been fully identified. CRISPR-Cas9 screens can identify host dependency factors essential for EMCV and enhance our understanding key events that follow infection, potentially new strategies...
Viral myocarditis, an inflammatory disease of the heart, causes significant morbidity and mortality. Type I interferon (IFN)-mediated antiviral responses protect against but mechanisms are poorly understood. We previously identified A Disintegrin And Metalloproteinase domain 9 (ADAM9) as important factor in viral pathogenesis. ADAM9 is implicated a range human diseases, including diseases; however, its role infection unknown. Here, we demonstrate that mice lacking more susceptible to...
Infection with the trematode parasite Schistosoma mansoni results in a distinct heterogeneity of disease severity, both humans and an experimental mouse model. Severe is characterized by pronounced hepatic egg-induced granulomatous inflammation proinflammatory cytokine environment, whereas mild corresponds reduced Th2 skewed environment. This marked indicates that genetic differences play significant role development, yet little known about basis dissimilar immunopathology. To investigate...
ABSTRACT In schistosomiasis mansoni, parasite eggs cause hepatointestinal granulomatous inflammation and fibrosis mediated by CD4 T cells specific for egg antigens. The severity of disease varies extensively in humans among mouse strains. Marked exacerbation induced typically low-pathology C57BL/6 mice immunization with schistosome antigens (SEA) complete Freund's adjuvant (SEA/CFA) correlates elevated production the proinflammatory cytokines gamma interferon (IFN-γ) interleukin-17 (IL-17),...
Encephalomyocarditis virus (EMCV) is a picornavirus that produces lytic infections in murine and human cells. Employing genome-wide CRISPR-Cas9 knockout screen to find host factors required for EMCV infection, we identified role ADAM9 infection. CRISPR-mediated deletion of multiple cell lines rendered the cells highly resistant infection death. Primary fibroblasts from KO mice were also strongly In contrast, WT equally susceptible with other viruses, including Coxsackie B. failed produce...
Abstract Profound vascular leakage in conjunction with elevated viremia is the hallmark of Dengue Hemorrhagic Fever/Dengue Shock Syndrome (DHF/DSS). Antibody (Ab)-dependent enhancement (ADE), which pre-existing, cross-reactive Ab enhance virus infectivity, thought to be responsible for increased viremia, while loss endothelial cell (EC) barrier integrity precursor plasma leakage. However, relationship between and leak has not been established. We hypothesize that DV-Ab immune complexes...