A5075633089- Ubiquitin and proteasome pathways
- Endoplasmic Reticulum Stress and Disease
- Metal complexes synthesis and properties
- Free Radicals and Antioxidants
- Enzyme-mediated dye degradation
- RNA modifications and cancer
- Genetics and Neurodevelopmental Disorders
- Chemical Synthesis and Analysis
- Microbial Metabolism and Applications
- Dialysis and Renal Disease Management
- Inflammatory mediators and NSAID effects
- Biochemical effects in animals
- Pesticide and Herbicide Environmental Studies
- Prion Diseases and Protein Misfolding
- Protein Interaction Studies and Fluorescence Analysis
- Protein Degradation and Inhibitors
- Alzheimer's disease research and treatments
- melanin and skin pigmentation
- Stress Responses and Cortisol
- Molecular Sensors and Ion Detection
- Lanthanide and Transition Metal Complexes
- Angiogenesis and VEGF in Cancer
- interferon and immune responses
- Multiple Myeloma Research and Treatments
- Toxoplasma gondii Research Studies
Institute of Crystallography
2019-2023
Consorzio Catania Ricerche
1993-2022
National Research Council
2017-2022
Yale University
2021
Institute of Biostructure and Bioimaging
2003-2018
University of Catania
1998-2018
Ospedale Sandro Pertini
2017
Sapienza University of Rome
1997-2012
Istituto Officina dei Materiali
2000
The aim of this study is to verify if water-soluble porphyrins can be used as proteasome inhibitors. We have found that cationic inhibit peptidase activities much more effectively than the corresponding anionic derivatives. relevance electrostatics in driving porphyin-proteasome interactions has been confirmed by observation inhibitory efficiency macrocycles decreases with number positive substituents. also investigated various metalloporphyrins, which differ due different propension central...
The tetraanionic meso-tetrakis(4-sulfonatophenyl)porphine (H2TPPS) in the pH range 5−12 exists a monomeric form, and its fluorescence is not pH-dependent. However, presence of polylysine, absorption, circular dichroism, resonance light scattering data indicate extensive polymer-induced self-aggregation porphyrins. In addition, intensity vs behavior deeply modified, showing sigmoidal profile. particular, at low pHs (≤7), protonated polylysine promotes porphyrins binding with consequent strong...
Due to their altered metabolism cancer cells are more sensitive proteasome inhibition or changes of copper levels than normal cells. Thus, the development complexes endowed with features has emerged as a promising anticancer strategy. However, limited information is available about exact mechanism by which inhibits proteasome. Here we show that Cu(II) ions simultaneously inhibit three peptidase activities isolated 20S proteasomes potencies (IC50) in micromolar range. ions, cell-free...
Type 2 Diabetes is a major public health threat, and its prevalence increasing worldwide. The abnormal accumulation of islet amyloid polypeptide (IAPP) in pancreatic β-cells associated with the onset disease. Therefore, design small molecules able to inhibit IAPP aggregation represents promising strategy development new therapies. Here we employ vitro, biophysical, computational methods inspect ability Silybin A B, two natural diastereoisomers extracted from milk thistle, interfere toxic...
An aqueous solution spectroscopic (Vis and EPR) study of the copper(II) complexes with Ac-HGGG-NH2 Ac-PHGGGWGQ-NH2 polypeptides (generically designated as L) suggests square base pyramids ascribable to [Cu(L)H−2] complex species, which contain three nitrogen donor atoms, arising from imidazole peptide groups, in equatorial plane for a pseudo-octahedral geometry case [Cu(L)H−3]− [Cu(L)H−4]2− have four atoms their plane. The coordination sphere copper is present at neutral pH values, completed...
Abstract The neuropeptide carnosine (β‐amyloid peptide aggregation has been demonstrated. Carnosine protection against peroxynitrite damage is particularly relevant, but until now there no evidence of any direct interaction with nitric oxide. In this study we examined the that provides oxide (NO)–induced cell death in primary rat astroglial cultures treated lipopolysaccharide (LPS) and interferon gamma (INFγ), a well‐known neurotoxic proinflammatory condition. A correlation was found between...
Three homologous cationic porphyrins differently affect the 20S proteasome gating mechanism.
A peptide belonging to the histidine–proline rich glycoprotein binds copper(<sc>ii</sc>), inhibiting metal angiogenic responses in endothelial cells.
The adsorption on hydrophobic and hydrophilic silica-based surfaces of the integrin-binding PHSRN peptide single-residual-mutated analogues, PHSEN PHSFN, is investigated by comparative QCM-D, XPS, SFG measurements molecular dynamics calculations. Endothelial cell cultures peptide-functionalized materials highlight their tunable pro- or anti-angiogenic potential.
Proteasome malfunction parallels abnormal amyloid accumulation in Alzheimer's Disease (AD). Here we scrutinize a small library of pyrazolones by assaying their ability to enhance proteasome activity and protect neuronal cells from toxicity. Tube tests evidenced that aminopyrine nifenazone behave as 20S activators. Enzyme assays carried out on an "open gate" mutant (α3ΔN) demonstrated activates through binding the α-ring surfaces influencing gating dynamics. Docking studies coupled with...