A5043908886- Silymarin and Mushroom Poisoning
- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- Synthesis and bioactivity of alkaloids
- RNA Interference and Gene Delivery
- Natural product bioactivities and synthesis
- Phytochemistry and Biological Activities
- Phytochemicals and Antioxidant Activities
- Cholinesterase and Neurodegenerative Diseases
- Enzyme function and inhibition
- Endoplasmic Reticulum Stress and Disease
- Alzheimer's disease research and treatments
- Berberine and alkaloids research
- Medicinal plant effects and applications
- Natural Antidiabetic Agents Studies
- Potato Plant Research
- Drug-Induced Hepatotoxicity and Protection
- Cancer-related gene regulation
- RNA modifications and cancer
- Synthesis and Characterization of Heterocyclic Compounds
- Analytical chemistry methods development
- Plant Pathogens and Resistance
- Food Quality and Safety Studies
- Curcumin's Biomedical Applications
- Polyamine Metabolism and Applications
University of Naples Federico II
2016-2025
Istituto Nazionale di Fisica Nucleare, Sezione di Napoli
2018
University of Sannio
2016
Methylation of adenine N6 (m6A) is the most frequent RNA modification. On mRNA, it catalyzed by METTL3–14 heterodimer complex, which plays a key role in acute myeloid leukemia (AML) and other types blood cancers solid tumors. Here, we disclose first proteolysis targeting chimeras (PROTACs) for an epitranscriptomics protein. For designing PROTACs, made use crystal structure complex with potent selective small-molecule inhibitor (called UZH2). The optimization linker started from desfluoro...
The self-assembling of the amyloid β (Aβ) peptide into neurotoxic aggregates is considered a central event in pathogenesis Alzheimer's disease (AD). Based on "amyloid hypothesis", many efforts have been devoted to designing molecules able halt progression by inhibiting Aβ self-assembly. Here, we combine biophysical (ThT assays, TEM and AFM imaging), biochemical (WB ESI-MS), computational (all-atom molecular dynamics) techniques investigate capacity four optically pure components natural...
Type 2 Diabetes is a major public health threat, and its prevalence increasing worldwide. The abnormal accumulation of islet amyloid polypeptide (IAPP) in pancreatic β-cells associated with the onset disease. Therefore, design small molecules able to inhibit IAPP aggregation represents promising strategy development new therapies. Here we employ vitro, biophysical, computational methods inspect ability Silybin A B, two natural diastereoisomers extracted from milk thistle, interfere toxic...
Rationale:Silybum marianum is used to protect against degenerative liver damage. The molecular mechanisms of its bioactive component, silybin, remained enigmatic, although membrane-stabilizing properties, modulation membrane protein function, and metabolic regulation have been discussed for decades. Methods: Experiments were performed with hepatocyte cell lines primary monocytes in vitro under both basal stressed conditions, mice vivo. Quantitative lipidomics was detect changes phospholipids...
Curcumin is recognized for its diverse biological activities, including the ability to induce apoptosis and ferroptosis. Therefore, it represents a promising candidate development of new compounds with neuroprotective anticancer properties. In order synthesize mimics improved pharmacokinetic properties (better solubility stability than curcumin) here, we present design synthesis novel curcumin analogues named Ethylphosphonate-based (EPs), which preserve pharmacophoric features curcumin. New...
The methanol extracts of the aerial part four ethnomedicinal plants Mediterranean region, two non-seed vascular plants, Equisetum hyemale L. and Phyllitis scolopendrium (L.) Newman, Spermatophyta, Juniperus communis (J. communis) Cotinus coggygria Scop. (C. coggygria), were screened against human cells lines (A549, MCF7, TK6 U937). Only J. C. showed marked cytotoxic effects, affecting both cell morphology growth. A dose-dependent effect these was also observed on cycle distribution....
We investigate the therapeutic potential of Aloin A and B, two natural compounds derived from Aloe vera leaves, focusing on their neuroprotective anticancer properties. The structural differences between these epimers suggest that they may exhibit distinct pharmacological Our investigations revealed both are not stable in aqueous solution tend to degrade rapidly, with concentration decreasing by over 50% within approximately 12 h. These results underscore importance addressing issues such as...
Silybin A and B were separated from commercial silibinin using the preparative HPLC method. The described method is rapid effective in obtaining gram-scale amounts of two diastereoisomers with minimal effort. In our approach, was dissolved THF (solubility greater than 100 mg/mL), an alternative solvent to H₂O or MeOH which has a very low solubility (ca 0.05-1.5 then into its components RP-HPLC. By starting purified diastereoisomers, it possible obtain enantiomers 2,3-dehydrosilybin good...
Alzheimer's disease (AD) is linked to the abnormal accumulation of amyloid β peptide (Aβ) aggregates in brain. Silybin B, a natural compound extracted from milk thistle (Silybum marianum), has been shown significantly inhibit Aβ aggregation vitro and exert neuroprotective properties vivo. However, further explorations silybin B's clinical potential are currently limited by three main factors: (a) poor solubility, (b) instability blood serum, (c) only partial knowledge silybin's mechanism...
By exploiting the regioselective protection of hydroxyl groups silibinin along with well-known phosphoramidite chemistry, we have developed an efficient strategy for synthesis new silibinin-modified species, which named Phosphate-Linked Silibinin Dimers (PLSd), in monomer units are linked by phosphodiester bonds. The antioxidant abilities PLSd were estimated on HepG2 cells using DPPH free radical scavenging and xanthine/xanthine oxidase assays. phosphate-metabolites showed a higher...
Epidemiological studies have proved that tomato consumption is associated with a lower risk of developing several diseases (for example, certain types cancers, cardiovascular diseases, macular degeneration, age-related eye disease). Many micronutrients and bioactive compounds are mainly present in peel seeds lost during the processing into sauce, purée, paste juice.The addition lyophilized powdered pomace enhances properties purée. In this paper we report chemical physicochemical...
In recent years, many studies have highlighted the consistent finding of tramadol (TRA) in effluents from wastewater treatment plants (WTPs) and also some rivers lakes both Europe North America, suggesting that TRA is removed by no more than 36% specific disinfection treatments. The extensive use this drug has led to environmental pollution water soil, up its detection growing plants. order expand knowledge about toxicity as well nature by-products (DBPs), a simulation waste chlorination...
Silybin is a natural compound extensively studied for its hepatoprotective, neuroprotective and anticancer properties. Envisioning the enhancement of silybin potential by suitable modifications in chemical structure, here, series new 7-O-alkyl silybins derivatives were synthesized Mitsunobu reaction starting from tyrosol-based phenols, such as tyrosol (TYR, 3), 3-methoxytyrosol (MTYR, 4), 3-hydroxytyrosol (HTYR, 5). This research sought to explore antioxidant properties eighteen their...