A5073974335- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- Bacterial Genetics and Biotechnology
- Bacterial biofilms and quorum sensing
- Vibrio bacteria research studies
- Antibiotic Resistance in Bacteria
- Plant Parasitism and Resistance
- Power Line Inspection Robots
- Space Satellite Systems and Control
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Underwater Vehicles and Communication Systems
- Immune Cell Function and Interaction
City University of Hong Kong
2021-2025
Southwest University
2019-2023
Kōchi University
2019-2020
Anhui Normal University
2018
Pseudomonas syringae, a Gram-negative plant pathogen, expresses multitudinous transcriptional regulators to control the type III secretion system (T3SS) and response diverse environmental challenges. Although mechanisms of virulence-associated P. syringae have been studied for decades, overall crosstalk underlying these is still elusive. Here, we identify five T3SS (EnvZ-OmpR, CbrAB2, PhoPQ, PilRS, MgrA), find that two-component systems EnvZ-OmpR CbrAB2 negatively regulate T3SS. To elucidate...
The transcription factor regulatory network in Pseudomonas aeruginosa is complex and involves multiple regulators that respond to various environmental signals physiological cues by regulating gene expression. However, the biological functions of at least half its 373 putative factors (TFs) remain uncharacterised. Herein, chromatin immunoprecipitation sequencing (ChIP-seq) was used investigate binding sites 172 TFs P. PAO1 strain. results revealed 81,009 significant peaks genome, more than...
The transcription factor regulatory network in Pseudomonas aeruginosa is complex and involves multiple regulators that respond to various environmental signals physiological cues by regulating gene expression. However, the biological functions of at least half its 373 putative factors (TFs) remain uncharacterised. Herein, chromatin immunoprecipitation sequencing (ChIP-seq) was used investigate binding sites 172 TFs P. PAO1 strain. results revealed 81,009 significant peaks genome, more than...
The transcription factor (TF) regulatory network in Pseudomonas aeruginosa is complex and involves multiple regulators that respond to various environmental signals physiological cues by regulating gene expression. However, the biological functions of at least half its 373 putative TFs remain uncharacterised. Herein, chromatin immunoprecipitation sequencing (ChIP-seq) was used investigate binding sites 172 P. PAO1 strain. results revealed 81,009 significant peaks genome, more than which were...
Abstract Pathogen genetic diversity varies in response to environmental changes. However, it remains unclear whether plant barriers invasion could be considered a bottleneck for phytopathogen populations. Here, we implement barcoding approach generate pool of 90 isogenic and individually barcoded Ralstonia solanacearum strains. We used these strains inoculate tomato plants with different degrees physical permeability (intact roots, wounded roots xylem inoculation) quantify the population...
A syringe-like type III secretion system (T3SS) plays essential roles in the pathogenicity of Ralstonia solanacearum, which is a causal agent bacterial wilt disease on many plant species worldwide. Here, we characterized functional CysB regulator (RSc2427) R. solanacearum OE1-1 that was demonstrated to be responsible for cysteine synthesis, expression T3SS genes, and solanacearum. The cysB mutants were auxotrophs failed grow minimal medium but grew slightly host plants. Supplementary...
Ralstonia solanacearum can metabolize ferulic acid (FA) and salicylic (SA), two representative phenolic acids, to protect it from toxicity of acids. Here, we genetically demonstrated a novel decarboxylase regulator (PadR)-like PrhP as positive on detoxification SA FA in R. solanacearum. Although the ability degrade enhances infection process toward host plants, greatly contributes besides degradation FA. Our results growth assay, promoter activity RNA-seq qRT-PCR revealed that plays multiple...
Sufficient nutrition contributes to rapid translational elongation and protein synthesis in eukaryotic cells prokaryotic bacteria. Fast accumulation of type III secretion system (T3SS) proteins conduce the invasion pathogenic bacteria into host cells. However, patterns T3SS under T3SS-inducing conditions remain unclear. Here, we report a mechanism regulators, effectors structural four model (Pseudomonas syringae, Pseudomonas aeruginosa, Xanthomonas oryzae Ralstonia solanacearum) clinical...
Abstract Type IV pili (T4P) are virulence factors in various pathogenic bacteria of animals and plants that play important roles twitching motility, swimming biofilm formation, adhesion to host cells. Here, we genetically characterized functional a putative T4P assembly protein TapV (Rsc1986 reference strain GMI1000) its homologue Rsp0189, which shares 58% amino acid identity with TapV, Ralstonia solanacearum . Deletion tapV , but not rsp0189 resulted significantly impaired tomato roots,...
Abstract The transcription factor regulatory network in Pseudomonas aeruginosa is complex and involves multiple regulators that respond to various environmental signals physiological cues by regulating gene expression. However, the biological functions of at least half its 373 putative factors (TFs) remain uncharacterised. Herein, chromatin immunoprecipitation sequencing (ChIP-seq) was used investigate binding sites 172 TFs P. PAO1 strain. results revealed 81,009 significant peaks genome,...
Ralstonia solanacearum RSc2741 has been predicted as a gamma-glutamyl phosphate reductase ProA catalyzing the second reaction of proline formation from glutamate. Here, we experimentally demonstrated that proA mutants were auxotrophs failed to grow in minimal medium, and supplementary proline, but not glutamate, fully restored diminished growth, confirming is responsible for biosynthesis glutamate R. solanacearum. was previously identified one candidates regulating expression genes type...
The shikimate pathway is a general route for the biosynthesis of aromatic amino acids (AAAs) in many microorganisms. A 3-dehydroquinase, AroQ, controls third step that catalyzes formation 3-dehydroquinate from 3-dehydroshikimate via trans-dehydration reaction. Ralstonia solanacearum harbors two 3-dehydroquinases, AroQ1 and AroQ2, sharing 52% similarity acids. Here, we demonstrated are essential R. solanacearum. growth was completely diminished nutriment-limited medium with deletion both...
RsgA plays an important role in maturation of 30S subunit many bacteria that assists the release RbfA from during a late stage ribosome biosynthesis. Here, we genetically characterized functional roles Ralstonia solanacearum, hereafter designated RsRsgA. Deletion R. solanacearum rsgA or rbfA resulted distinct deficiency 16S ribosomal RNA, significantly slowed growth broth medium, and diminished nutrient-limited which are similar as phenotypes mutants Escherichia coli other bacteria. Our...
Ralstonia solanacearum RSc0454 is predicted as a FAD-linked oxidase based on protein homologies, while it contains distinct domains of lactate dehydrogenase and succinate dehydrogenase. A previous study demonstrated that exhibits activity using pyruvate NADH substrates, essential for pathogenicity R. solanacearum. Here, we genetically characterized involvement bacterial growth expression genes the type III secretion system (T3SS, determinant) in The mutant grew normally rich medium but...