A5002239223- Monoclonal and Polyclonal Antibodies Research
- Pain Mechanisms and Treatments
- Multiple Myeloma Research and Treatments
- Food Allergy and Anaphylaxis Research
- HER2/EGFR in Cancer Research
- CAR-T cell therapy research
- Neuropeptides and Animal Physiology
- Chronic Lymphocytic Leukemia Research
- Allergic Rhinitis and Sensitization
- Cancer Treatment and Pharmacology
- Genetically Modified Organisms Research
- Cannabis and Cannabinoid Research
- Glycosylation and Glycoproteins Research
- Exercise and Physiological Responses
- Microtubule and mitosis dynamics
- Immunotoxicology and immune responses
- Consumer Attitudes and Food Labeling
- Pesticide Exposure and Toxicity
- Seed and Plant Biochemistry
- Transgenic Plants and Applications
- Meat and Animal Product Quality
- Drug Transport and Resistance Mechanisms
- Adenosine and Purinergic Signaling
- Clostridium difficile and Clostridium perfringens research
- Pesticide and Herbicide Environmental Studies
Genesys (United States)
2013
University of Arizona
2006-2011
University of Missouri
2010
Monsanto (United States)
2010
Pioneer Hi-Bred
2010
Syngenta (United States)
2010
Background Exercise is often prescribed as a therapy for chronic pain. Short-term exercise briefly increases the production of endogenous analgesics, leading to transient antinociception. In limited studies, produced sustained in opioids, analgesia, or diminished measures This study tests hypothesis that regular aerobic leads reversal neuropathic pain by activating opioid-mediated modulatory systems. Methods After baseline measurements, L5 and L6 spinal nerves male Sprague-Dawley rats were...
The anti-FcRH5/CD3 T cell-dependent bispecific antibody (TDB) targets the B cell lineage marker FcRH5 expressed in multiple myeloma (MM) tumor cells. We demonstrate that TDBs trigger receptor activation by inducing target clustering and exclusion of CD45 phosphatase from synapse. dimensions molecule play a key role efficiency synapse formation. TDB kills human plasma cells patient-derived at picomolar concentrations results complete depletion bone marrow cynomolgus monkeys. These data...
Management of acute pain remains a significant clinical problem. In preclinical studies, CB2 cannabinoid receptor-selective agonists inhibit nociception without producing central nervous system side effects. The agonist AM1241 produces antinociceptive effects that are antagonized by CB2, but not CB1, antagonists, suggesting activation receptors results in antinociception. However, it has been possible to definitively demonstrate these mediated receptors, because we have lacked the...
Soybean (Glycine max) seed contain some proteins that are allergenic to humans and animals. However, the concentration of these allergens their expression variability among germplasms is presently unknown. To address this problem, 10 were quantified from 20 nongenetically modified commercial soybean varieties using parallel, label-free mass spectrometry approaches. Relative quantitation was performed by spectral counting absolute multiple reaction monitoring (MRM) with synthetic,...
B7-H4 has been implicated in cancers of the female reproductive system and investigated for its possible use as a biomarker cancer, but there are no preclinical studies to demonstrate that is molecular target therapeutic intervention cancer. We provide evidence prevalence expression levels high different subtypes breast cancer only few normal tissues express on cell membrane. These profiles low upregulation an opportunity antibody–drug conjugates (ADCs), cytotoxic drugs chemically linked...
Abstract The clinically important opioid fentanyl, administered acutely, enhances mechanical hypersensitivity in a model of surgical pain induced by plantar incision. Activity neurokinin‐1 (NK‐1) receptor‐expressing ascending spinal neurons, descending pathways originating the rostral ventromedial medulla (RVM), and dynorphin are necessary for development maintenance hyperalgesia during sustained morphine exposure, suggesting that these mechanisms may also be enhancement pain. Therefore, we...
Oxyfluorfen is a herbicide that not genotoxic and produces liver toxicity in rodents, following repeated administration at high dose levels. Lifetime rodent feeding studies reported 1977 with low-purity oxyfluorfen (85%) showed no increase any tumor type rats (800 ppm, dose) only marginally increased incidence of hepatocellular tumors male CD-1 mice the highest (200 ppm). To evaluate potential carcinogenicity currently registered (> 98% purity), we conducted series short-term mode action...
A number of cytotoxic pyrrolobenzodiazepine (PBD) monomers containing various disulfide-based prodrugs were evaluated for their ability to undergo activation (disulfide cleavage) in vitro the presence either glutathione (GSH) or cysteine (Cys). good correlation was observed between GSH stability and cytotoxicity toward tumor cell lines. The prodrug-containing compounds typically more potent against cells with relatively high intracellular levels (e.g., KPL-4 cells). Several antibody–drug...
This work discloses the first examples of antibody-drug conjugates (ADCs) that are constructed from linker-drugs bearing dimeric seco-CBI payloads (duocarmycin analogs). Several homogeneous, CD22-targeting THIOMAB (TDCs) containing entities shown to be highly efficacious in WSU-DLCL2 and BJAB mouse xenograft models. Surprisingly, seco-CBI-containing also observed undergo significant biotransformation vivo mice, rats, monkeys thereby form 1:1 adducts with Alpha-1-Microglobulin (A1M) plasma...
Bispecific antibody production using single host cells has been a new advancement in the engineering field. We previously showed comparable vitro biological activity and vivo mouse pharmacokinetics (PK) for two novel cell variants (v10 v11) one traditional dual vitro-assembled anti-human epidermal growth factor receptor 2/CD3 T-cell dependent bispecific (TDB) antibodies. Here, we extended our previous work to assess cell-produced of TDB against FcRH5, B-cell lineage marker expressed on...
Several factors were studied as affecting protein degradation and texture of skipjack tuna muscle following ambient pressure thermal processing (precooking). These included degree mushy syndrome (MTS) evidenced in the raw meat, meat pH, abusive thawing/holding, precooking temperature/time. Slurries intact pieces from frozen tuna, either tempered for 2 h or thawed held at 25 °C 22 (abusive treatment) heated temperatures ranging 40 to 80 up h, also 90 1 with without prior adjustment pH 5 7...
A workshop entitled "Challenges and Opportunities in Evaluating Protein Allergenicity across Biotechnology Industries" was held at the 51st Annual Meeting of Society Toxicology (SOT) San Francisco, California. The sponsored by Specialty Section SOT designed to present science-based approaches used biotechnology industries evaluate regulate protein allergenicity. panel experts from industry government highlighted allergenicity testing requirements research agricultural,...
SMARCA2 and SMARCA4 are the ATPases of SWI/SNF chromatin remodeling complex, which play a significant role in regulating transcriptional activity DNA repair cells. has become an appealing synthetic-lethal, therapeutic target oncology, as mutational loss many cancers leads to functional dependency on residual activity. Thus, for development, important step is understanding any potential safety target-associated liabilities inhibition. To best mimic therapeutic, tamoxifen-inducible (TAMi)...
Nonclinical toxicology studies are conducted to characterize the potential toxicities and establish a safe starting dose for new drugs in clinical studies, but question remains as how predictable/translatable nonclinical safety findings humans. In many cases, there is good concordance between species patients. However, cases which lack of predictivity or translatability that led early termination due unanticipated programs before making it clinic unacceptable assumed be translatable. A few...