A5032927564- Cancer-related molecular mechanisms research
- Advanced biosensing and bioanalysis techniques
- Circular RNAs in diseases
- MicroRNA in disease regulation
- Biosensors and Analytical Detection
- RNA modifications and cancer
- Analytical chemistry methods development
- RNA Research and Splicing
- Coronary Interventions and Diagnostics
- Antiplatelet Therapy and Cardiovascular Diseases
- Natural product bioactivities and synthesis
- Cancer, Hypoxia, and Metabolism
- Chromatography in Natural Products
- Ubiquitin and proteasome pathways
- Pharmacological Effects of Natural Compounds
- Pesticide Residue Analysis and Safety
- Pregnancy and preeclampsia studies
- RNA Interference and Gene Delivery
- Electrochemical sensors and biosensors
- Extracellular vesicles in disease
- Adenosine and Purinergic Signaling
- Ginseng Biological Effects and Applications
- Metabolomics and Mass Spectrometry Studies
- Advanced Nanomaterials in Catalysis
- Neonatal and Maternal Infections
Nanjing Medical University
2015-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2025
Jianghan University
2016-2024
Chinese Academy of Agricultural Sciences
2019-2024
Institute of Quality Standards and Testing Technology for Agro Products
2019-2024
WuXi AppTec (China)
2018-2024
Changzhou No.2 People's Hospital
2023
Shanghai Institute of Materia Medica
2021
Chinese Academy of Sciences
2021
Nanjing Jiangning Hospital
2020-2021
MicroRNAs are a class of small noncoding RNAs that function as critical gene regulators through targeting mRNAs for translational repression or degradation. In this study, we showed miR-128 expression levels were decreased in glioma, and identified p70S6K1 novel direct target miR-128. Overexpression suppressed its downstream signaling molecules such HIF-1 VEGF expression, attenuated cell proliferation, tumor growth angiogenesis. Forced can partly rescue the inhibitory effect cells. Taken...
The biomimetic enzyme-linked immunosorbent assay (BELISA) is widely used for detection of small-molecule compounds as a result low cost and reagent stability molecularly imprinted polymers (MIPs). However, enzyme labels in BELISA still suffer some drawbacks, such high production limited stability. To overcome the nanozyme-linked (BNLISA) based on MIPs nanozyme was first proposed. For labels, platinum nanoparticles (PtNPs) acted peroxidase by catalyzing oxidation colorless...
A novel strategy for the fabrication of electrochemical aptasensor is proposed in this work, and has been employed to develop an assay adenosine deaminase activity. While a well-designed oligonucleotide containing three functional regions (an aptamer region, G-quadruplex halves linker region) adopted our as core element, enzymatic reaction catalyzed by plays key role well regulation binding with hemin, electroactive probe, which reflect activity enzyme indirectly but accurately. The...
The testes are where spermatogenesis, the sperm-generating process that is unique to men, occurs. Importantly, human spermatogenesis and tumorigenesis share key similarities. Until now, only a few proteins in testis have been identified due limitations of available technology. In this paper, using an advanced proteomics platform, we 7346 within with high degree confidence. Immunohistochemistry data from Human Protein Atlas database show over 90% (1833/2020) can be detected specific...
As one of most common synthetic phenolic antioxidants, tertiary butylhydroquinone (TBHQ) has received increasing attention due to the potential risk for liver damage and carcinogenesis. Herein, a simple rapid fluorescent switchable methodology was developed highly selective sensitive determination TBHQ by utilizing competitive interaction between photoinduced electron transfer (PET) effect carbon dots (CDs)/Fe(III) ions complexation reaction TBHQ/Fe(III) ions. This novel sensing platform...
Abstract Brahma-related gene-1 (BRG1) is the specific ATPase of switch/sucrose nonfermentable chromatin-remodeling complex that aberrantly expressed or mutated in various cancers. However, exact role BRG1 oncogenesis remains unknown. In this study, we demonstrate knockdown (KD) promotes cellular senescence by influencing SIRT1/p53/p21 signal axis colorectal cancer (CRC). particular, reveal expression level inversely correlated with p21, one classic regulators, and decreased senescent CRC...
VEGF receptor-2 (VEGFR-2 or kinase insert domain receptor; KDR) is a known endothelial target also expressed in NSCLC tumor cells. We investigated the association between alterations KDR gene and clinical outcome patients with resected non-small-cell lung carcinoma (NSCLC; n = 248). copy number gains (CNG), measured by quantitative PCR fluorescence situ hybridization, were detected 32% of tumors associated significantly higher protein microvessel density than without CNGs. CNGs increased...
Preeclampsia (PE) is a syndrome manifested by high blood pressure that could develop in the latter half of pregnancy; however, underlying mechanisms are not understood. Recent evidence points to function noncoding RNAs (ncRNAs) as novel regulators invasion, migration, proliferation, and apoptosis trophoblasts involved development placental vasculature. Here, we investigated role long intergenic ncRNA 00473 (linc00473) PE associated molecular mechanisms. The expression linc00473 was...
Dysregulated expression of long non-coding RNAs (lncRNAs) has been reported in many types cancers, indicating that it important regulatory roles human cancer biology. Recently, lncRNA urothelial cancer-associated 1 (UCA1) was shown to be dysregulated types, but the detailed mechanisms remain largely unknown. In our study, we found upregulated UCA1 is associated with poor prognosis gastric patients. Further experiments revealed knockdown significantly repressed proliferation and migration...
Chronic inflammatory and immune dysregulation are critical drivers of the development progression chronic obstructive pulmonary disease (COPD). Posttranslational modifications, such as glycosylation Immunoglobulin G (IgG), crucial in modulating systemic homeostasis. This study aims to profile plasma IgG glycopeptides (IgGPs) COPD patients uncover new insights into their pathogenesis identify novel biomarkers. An integrated platform that combines Fe3O4@PDA@DETA nanospheres enrichment with...
Sarcopenia, characterized by an age-related decline in skeletal muscle mass and function, is closely linked to vitamin D deficiency. This study examines the role of Sirtuin 1 (Sirt1) its regulation preventing sarcopenia. Utilizing wild-type, 1α-hydroxylase knockout (1α(OH)ase−/−), Sirt1 transgenic (Sirt1Tg) 1α(OH)ase−/− mice, we investigated levels, mass, fiber type, senescence markers. Our results demonstrated that 1,25-Dihydroxyvitamin (1,25(OH)2D3) upregulated myogenic factor MyoD1...
Autotaxin (ATX) possesses lysophospholipase D (lyso PLD) activity, which converts lysophosphatidylcholine (LPC) into lysophosphatidic acid (LPA). The ATX-LPA signaling axis has been implicated in angiogenesis, chronic inflammation and tumor progression. Osteopontin (OPN) is an important chemokine involved the survival, proliferation, migration, invasion metastasis of gastric cancer cells. focus present study was to investigate relationship between OPN. In comparison with non-treated cells,...
A number of cytotoxic pyrrolobenzodiazepine (PBD) monomers containing various disulfide-based prodrugs were evaluated for their ability to undergo activation (disulfide cleavage) in vitro the presence either glutathione (GSH) or cysteine (Cys). good correlation was observed between GSH stability and cytotoxicity toward tumor cell lines. The prodrug-containing compounds typically more potent against cells with relatively high intracellular levels (e.g., KPL-4 cells). Several antibody–drug...
Abstract Introduction – Centrifugal partition chromatography (CPC), as a continuous liquid–liquid with no solid support matrix, combined evaporative light scattering detection (ELSD) was employed for systematic separation and purification of weak‐chromophoric saponins from highly valued important traditional Chinese herbal medicine, Panax notoginseng . Objective To separate isolate high‐purity extract using CPC‐ELSD simple low toxicity solvent system. Methodology Samples were preparaed by...
In recent years, the identification of long non-coding RNAs (lncRNAs) led to analysis their characteristics in cancer biology. However, expression lncRNAs and clinical significance remain unclear. present study, an investigation that may be involved regulation metastasis using microarray polymerase chain reaction analyses resulted MLLT4 antisense RNA 1 (MLLT4‑AS1) as a significantly downregulated lncRNA gastric tissue compared with normal adjacent (P=0.006). Furthermore, downregulation...
Accumulating evidence supports the importance of noncoding RNAs and exosomes in coronary heart disease (CHD). However, exosomal-associated competing endogenous RNA- (ceRNA-) mediated regulatory mechanisms CHD are largely unexplored. The present study aimed to explore ceRNA networks CHD.Data from 6 patients 32 normal controls were downloaded ExoRBase database. compared by screening differentially expressed mRNAs (DEMs), lncRNAs (DELs), circRNAs (DECs) serum exosomes. MicroRNAs (miRNAs)...