A5041190147- Hedgehog Signaling Pathway Studies
- Tissue Engineering and Regenerative Medicine
- Extracellular vesicles in disease
- Cardiac Fibrosis and Remodeling
- Mesenchymal stem cell research
- Telomeres, Telomerase, and Senescence
- RNA Interference and Gene Delivery
- Cardiac Ischemia and Reperfusion
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Nerve injury and regeneration
- Neurogenesis and neuroplasticity mechanisms
- Nitric Oxide and Endothelin Effects
- Genetics, Aging, and Longevity in Model Organisms
- Axon Guidance and Neuronal Signaling
- Electrospun Nanofibers in Biomedical Applications
- Cardiomyopathy and Myosin Studies
- Developmental Biology and Gene Regulation
- Angiogenesis and VEGF in Cancer
- Biomedical and Engineering Education
- Interdisciplinary Research and Collaboration
- MicroRNA in disease regulation
- Cardiac Structural Anomalies and Repair
- Atherosclerosis and Cardiovascular Diseases
- Circular RNAs in diseases
Northwestern University
2008-2020
Cardiovascular Institute of the South
2017
Icahn School of Medicine at Mount Sinai
2017
Neostem (United States)
2017
Cardiovascular Institute Hospital
2016
Tokyo Women's Medical University
2012
Osaka Medical and Pharmaceutical University
2012
Medizinische Hochschule Hannover
2012
Northwestern Memorial Hospital
2012
Inserm
2009
Rationale: Paracrine secretions seem to mediate therapeutic effects of human CD34 + stem cells locally transplanted in patients with myocardial and critical limb ischemia animal models. Earlier, we had discovered that paracrine secretion from contains proangiogenic, membrane-bound nanovesicles called exosomes (CD34Exo). Objective: Here, investigated the mechanisms CD34Exo-mediated ischemic tissue repair angiogenesis by studying their miRNA content uptake. Methods Results: When injected into...
We hypothesized that a small molecule CXCR4 antagonist, AMD3100 (AMD), could augment the mobilization of bone marrow (BM)-derived endothelial progenitor cells (EPCs), thereby enhancing neovascularization and functional recovery after myocardial infarction. Single-dose AMD injection administered onset infarction increased circulating EPC counts vascularity, reduced fibrosis, improved cardiac function survival. In mice transplanted with traceable BM cells, BM-derived cell incorporation in...
Ischemic cardiovascular disease represents one of the largest epidemics currently facing aging population. Current literature has illustrated efficacy autologous, stem cell therapies as novel strategies for treating these disorders. The CD34+ hematopoetic shown significant promise in addressing myocardial ischemia by promoting angiogenesis that helps preserve functionality ischemic myocardium. Unfortunately, both viability and angiogenic quality autologous cells decline with advanced age...
Humans with a rare gene mutation in SERPINE1 live longer and show evidence of protection from aging-related morbidity.
Background— CXC-chemokine receptor 4 (CXCR4) regulates the retention of stem/progenitor cells in bone marrow (BM), and CXCR4 antagonist AMD3100 improves recovery from coronary ligation injury by mobilizing BM to peripheral blood. Thus, we investigated whether also ischemia/reperfusion injury, which more closely mimics myocardial infarction patients, because blood flow is only temporarily obstructed. Methods Results— Mice were treated with single subcutaneous injections (5 mg/kg) or saline...
Background— Bicuspid aortic valve (BAV) is a heritable condition that has been linked by an unknown mechanism to predisposition for ascending aneurysm. Matrix metalloproteinases have implicated in this predisposition. Metallothionein poorly characterized, metal-binding protein regulates matrix and antioxidant known be upregulated under oxidative stress. Methods Results— To determine putative factors involved the pathogenesis of aneurysm BAV patients, our first goal was identify genes are...
The Gli transcription factors are mediators of Hedgehog (Hh) signaling and have been shown to play critical roles during embryogenesis. Previously, we demonstrated that the Hh pathway is reactivated by ischemia in adult mammals, this can be stimulated for therapeutic benefit; however, specific ischemia-induced not elucidated.To investigate role Gli3 ischemic tissue repair.Gli3-haploinsufficient (Gli3(+/-)) mice their wild-type littermates were physiologically similar absence ischemia;...
Abstract Acute myocardial infarction induces activation of the acute phase response and infiltration leukocytes to infarcted area. Moreover, myocardium that is remote from ischemic area also becomes inflamed. Inflammatory reaction clears dead cells matrix debris, while prolongation or expansion inflammatory results in dysfunction following infarction. Wnt glycolipoproteins are best characterized as regulators embryonic development. Recently several reports suggest they contribute adult...
We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from non-affected control subjects to use as comparison group for iPSC lines containing Plasminogen Activator Inhibitor-1 (PAI-1 homozygous/heterozygous) mutation. The Sendai Virus (SeV) vector encoding Yamanaka transcription factors was used at low multiplicity of infection reprogram UCs.
Abstract Introduction: The National Academies of Sciences (NAS) emphasize the need for interdisciplinary team science (TS) training, but few training resources are available. COALESCE, an open-access tool developed with Institutes Health support and located at teamscience.net , is considered a gold standard resource has not previously been evaluated. COALESCE launched four learning modules in 2011. Science TS (SciTS) module, interactive encyclopedia, introduces foundational concepts. Three...
We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from subjects heterozygous for novel Plasminogen Activator Inhibitor-1 (PAI-1) mutation. The Sendai Virus (SeV) vector encoding Yamanaka transcription factors was used at low multiplicity of infection to reprogram PAI-1 UCs.
We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from subject with novel homozygous Plasminogen Activator Inhibitor-1 (PAI-1 null) mutation. The Sendai virus (SeV) vector encoding Yamanaka transcription factors was used at low multiplicity of infection to reprogram PAI-1 UCs.
Gli transcription factors are mediators of hedgehog signaling and have been shown to be critical in several steps during development. In addition, also implicated as potent oncogenes. We that the Hedgehog pathway is reactivated adult cardiovascular system under ischemic conditions can exploited therapeutically disease; however role expression response injury following ischemia not characterized. induced hind-limb by resection left femoral artery FVB mice found Gli2 Gli3 mRNA were...
Cell-based therapies have emerged as a promising option in ischemic tissue repair. Impaired viability and retention of injected cells the target zone reduced biopotency autologous from pts with advanced disease remain significant challenges. Advances bionanotechnology led to development biomaterials that may offer solutions overcome limitations enhance efficacy cell therapy by modulating microenvironment. Interaction fibronectin (FN) has been shown effect survival, adhesion motility. We...