A5072534750- Neuroscience and Neuropharmacology Research
- Nerve injury and regeneration
- RNA Research and Splicing
- Ion channel regulation and function
- Ubiquitin and proteasome pathways
- Epilepsy research and treatment
- Muscle Physiology and Disorders
- RNA regulation and disease
- Mitochondrial Function and Pathology
- Adenosine and Purinergic Signaling
University of Coimbra
2017-2024
Abstract Brain-derived neurotrophic factor (BDNF) is an important mediator of long-term synaptic potentiation (LTP) in the hippocampus. The local effects BDNF depend on activation translation activity, which requires delivery transcripts to synapse. In this work, we found that neuronal activity regulates dendritic localization RNA-binding protein heterogeneous nuclear ribonucleoprotein K (hnRNP K) cultured rat hippocampal neurons by stimulating BDNF-Trk signaling. Microarray experiments...
The RNA binding protein hnRNP K mediates BDNF-induced proteome remodeling at the neuronal synapse.
Cell culture models are important tools to study epileptogenesis mechanisms. The aim of this work was characterize the spontaneous and synchronized rhythmic activity developed by cultured hippocampal neurons after transient incubation in zero Mg2+ model Status Epilepticus. Cultured were transiently incubated with a Mg2+-free solution neuronal networks evaluated using single cell calcium imaging whole-cell current clamp recordings. Here we report development [Ca2+]i transients immediately...
Abstract Brain-derived neurotrophic factor (BDNF) is a key mediator of synaptic plasticity and memory formation in the hippocampus. However, BDNF-induced alterations glutamate receptors coupled to glutamatergic synapses hippocampus have not been elucidated. In this work we investigated putative role GluN2B-containing NMDA induced by BDNF. Stimulation hippocampal synaptoneurosomes with BDNF led significant time-dependent increase surface expression as determined immunocytochemistry...
The synaptic expression of glutamate receptors the α-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA) type is dynamically controlled by interaction with binding partners and auxiliary proteins. These proteins can be regulated posttranslational modifications, including ubiquitination. In this work, we investigated regulation receptor interacting protein-associated protein 1 (GRASP1) ubiquitin-dependent mechanisms its impact on surface activity AMPA receptors. Cotransfection...