A5000585760- Genomics and Phylogenetic Studies
- Yersinia bacterium, plague, ectoparasites research
- Vibrio bacteria research studies
- Plant Disease Resistance and Genetics
- Microbial Natural Products and Biosynthesis
- Bacterial biofilms and quorum sensing
- Legume Nitrogen Fixing Symbiosis
- Plant Pathogenic Bacteria Studies
- Antibiotic Resistance in Bacteria
- Microbial Community Ecology and Physiology
- Bacterial Genetics and Biotechnology
- Cassava research and cyanide
- Algal biology and biofuel production
- Microbial Metabolism and Applications
- Parasitic infections in humans and animals
- Religion, Society, and Development
- Viral Infections and Outbreaks Research
- Plant-Microbe Interactions and Immunity
- Legionella and Acanthamoeba research
- Viral Infections and Vectors
- interferon and immune responses
- Parasite Biology and Host Interactions
- Trace Elements in Health
- Bacterial Infections and Vaccines
- Plant-based Medicinal Research
Northwest A&F University
2014-2025
The type VI secretion system was recently reported to be involved in zinc acquisition, but the underlying mechanism remains unclear. Here, we report that Burkholderia thailandensis T6SS4 is acquisition via of a zinc-scavenging protein, TseZ, interacts with outer membrane heme transporter HmuR. We find HmuR redox-regulated dual-functional transports iron under normal conditions upon sensing extracellular oxidative stress, triggered by formation an intramolecular disulfide bond. Acting as...
Abstract Outer membrane vesicles (OMVs) can function as nanoscale vectors that mediate bacterial interactions in microbial communities. How bacteria recognize and recruit OMVs inter-specifically remains largely unknown, thus limiting our understanding of the complex physiological ecological roles OMVs. Here, we report a ligand-receptor interaction-based OMV recruitment mechanism, consisting type VI secretion system (T6SS)-secreted lipopolysaccharide (LPS)-binding effector TeoL outer...
Significance Although emerging evidence suggests that the STING-mediated immune response pathway plays a crucial role in microbial pathogen infection, few bacterial effectors have been reported to target this pathway. Here, we identified T6SS-secreted micropeptide, TssS, which is for pathogenesis of Yptb . Distinct from traditional host proteins or other macromolecules, TssS inhibits STING oligomerization and downstream signaling pathways by chelating Mn 2+ Thus, mediates previously...
ABSTRACT Urease converts urea into ammonia and carbon dioxide, providing a nitrogen source for microbial growth serving as an important mechanism human bacterial pathogens to survive in acidic conditions, which can be regulated by many factors. As global regulator, the ferric uptake regulator (Fur) regulates series of genes pathways involved different cellular processes virulence enteric bacterium Yersinia pseudotuberculosis ( Yptb ). However, whether Fur urease activity was still unknown....
Aerobactin is a citrate-hydroxamate siderophore that critical for the virulence of pathogenic enteric bacteria. However, although aerobactin-producing iucABCD - iutA operon distributed widely in genomes Yersinia species, none spp. was found to produce aerobactin. Here, we showed food-borne pathogen pseudotuberculosis YPIII functional system involved iron acquisition. The expression be directly repressed by ferric uptake regulator (Fur) an concentration-dependent manner. In addition,...
A yellow-pigmented bacterium, designated strain ZFGT-11(T), was isolated from roots of Geum aleppicum Jacq. collected Taibai Mountain in Shaanxi Province, north-west China, and subjected to a taxonomic study by using polyphasic approach. Cells ZFGT-11(T) were Gram-stain-negative, strictly aerobic rods that surrounded thick capsule motile means single polar flagellum. Phylogenetic analyses based on 16S rRNA gene sequences showed member the genus Sphingomonas closely related naasensis KACC...
Many bacteria secrete siderophores to enhance iron uptake under iron-restricted conditions. In this study, we found that Cupriavidus necator JMP134, a well-known aromatic pollutant-degrading bacterium, produces an unknown carboxylate-type siderophore named cupriabactin overcome limitation. Using genome mining, targeted mutagenesis, and biochemical analysis, discovered operon containing six open reading frames (cubA-F) in the C. JMP134 encodes proteins required for biosynthesis of...
Abstract Bacteria have evolved multiple secretion systems for delivering effector proteins into the cytosol of neighboring cells, but roles many these effectors remain unknown. Here, we show that Yersinia pseudotuberculosis secretes an effector, CccR, can act both as a toxin and transcriptional factor. The is secreted by type VI system (T6SS) enter nearby cells same species other (such Escherichia coli ) via cell-cell contact in contact-independent manner. CccR contains N-terminal FIC domain...
The type VI secretion system (T6SS) is a widespread protein apparatus deployed by many Gram-negative bacterial species to interact with competitor bacteria, host organisms, and the environment. Yersinia pseudotuberculosis T6SS4 was recently reported be involved in manganese acquisition; however, underlying regulatory mechanism still remains unclear. In this study, we discovered that regulated ferric uptake regulator (Fur) response ions (Mn
A Gram-reaction-negative, rod-shaped, non-motile, red-pigmented bacterial strain, designated ZLD-7(T), was isolated from a soil sample collected an arid area in Xinjiang Province north-west China, and characterized by using polyphasic taxonomic approach. 16S rRNA gene sequence analysis showed that strain ZLD-7(T) member of the genus Pontibacter family Cytophagaceae, with similarities 93.7-96.2 % to type strains other species. The only isoprenoid quinone MK-7, its major cellular fatty acids...
A yellowish-pigmented bacterium, designated strain PLGR-1(T), was isolated from the root of Bergenia scopulosa collected Taibai Mountain in Shaanxi Province, north-west China, and subjected to a taxonomic study by using polyphasic approach. Cells PLGR-1(T) were Gram-stain-negative, strictly aerobic, rod-shaped, non-spore-forming motile with single polar flagellum. Growth occurred at 7-33 °C (optimum, 25-28 °C), pH 5.0-10.0 6.0-7.0) 0-0.5 % (w/v) NaCl 0 %). The predominant respiratory quinone...
The transition between the planktonic state and biofilm-associated is a key developmental decision for pathogenic bacteria. Biofilm formation by Yersinia pestis regulated hmsHFRS genes (β-1, 6-N-acetyl-D-glucosamine synthesis operon) in its flea vector vitro. However, mechanism of biofilm pseudotuberculosis remains elusive. In this study, we demonstrate that LysR-type regulator RovM inversely regulates motility Y. acting as transcriptional these two functions. strongly induced during growth...
A strictly aerobic, light-yellow-coloured, stalked bacterium, designated strain ZFGT-14(T), was isolated from the root of Geum aleppicum Jacq. collected Taibai Mountain in Shaanxi province, north-west China, and subjected to a taxonomic study using polyphasic approach. This novel isolate grew at 7-33 °C (optimum 25-28 °C) pH 6.0-10.0 7.0-8.0). Flexirubin-type pigments were not produced. Cells Gram-stain-negative, rod-shaped motile with single polar flagellum. The predominant respiratory...
Microbial species often occur in complex communities and exhibit intricate synergistic antagonistic interactions. To avoid predation compete for favorable niches, bacteria have evolved specialized protein secretion systems. The type VI system (T6SS) is a versatile widely distributed among Gram-negative that translocates effectors into target cells or the extracellular milieu via various physiological processes. Pseudomonas aeruginosa an opportunistic pathogen responsible many diseases, it...
ABSTRACT The formation of reactive oxygen species is harmful and can destroy intracellular macromolecules such as lipids, proteins, DNA, even leading to bacterial death. To cope with this situation, microbes have evolved a variety sophisticated mechanisms, including antioxidant enzymes, siderophores, the type VI secretion system (T6SS). However, mechanism oxidative stress resistance in Cupriavidus pinatubonensis unclear. In study, we identified Reut_A2805 an OxyR ortholog C . , which...
A yellow-pigmented bacterium, designated strain GZJT-2T, was isolated from the stem of Hylomecon japonica (Thunb.) Prantl et Kündig collected Taibai Mountain in Shaanxi Province, north-west China. Cells GZJT-2T were Gram-reaction-negative, strictly aerobic, rod-shaped, non-spore-forming and non-motile. Phylogenetic analyses based on 16S rRNA gene sequences showed that a member genus Sphingomonas, with sequence similarities 92.1-96.9 % to type strains recognized species Sphingomonas (92.1...
Urease acts as an important acid resistance system and virulence factor that is widespread among microorganisms. RovM a global regulator regulates series of genes pathways including survival systems in the enteric bacterium Yersinia pseudotuberculosis (Yptb). However, whether urease activity Yptb was still unknown. In this study, by using qualitative quantitative assays, we show expression responds to nutrient conditions protein represses binding its promoter. A previously reported positive...
Metal ions are essential nutrients for all life forms, and restriction of metal ion availability is an effective host defense against bacterial infection. Meanwhile, pathogens have developed equally means to secure their supply. The enteric pathogen Yersinia pseudotuberculosis was found uptake zinc using the T6SS4 effector YezP, which Zn
Abstract The golden snub-nosed monkey ( Rhinopithecus roxellana ) is an endangered species endemic to China. Relatively little known about the taxonomic status of soil-transmitted helminths (STH) in these monkeys. Trichuris spp. (syn. Trichocephalus are among most important STHs, causing significant socio-economic losses and public health concerns. To date, five have been reported monkeys, including a novel species, T. rhinopiptheroxella, based on morphology. In present study, molecular...
A bacterial strain, designated as ZFJT-2T, was isolated from the stem of Geum aleppicum Jacq. collected Taibai Mountain in Shaanxi Province, north-west China. Cells strain ZFJT-2T were Gram-stain-negative, strictly aerobic, rod-shaped and motile by means a single polar flagellum. The major fatty acids summed feature 8 (comprising C18 : 1 ω7c and/or ω6c), C16 0, 11-methyl 3 DNA G+C content 58.3 mol% (HPLC). Phylogenetic analyses based on 16S rRNA gene sequences showed that member genus...
Nafulsella turpanensis ZLM-10(T) is a slightly halophilic, Gram-negative, rod-shaped, gliding, pale-pink-pigmented bacterium in the family Flammeovirgaceae, and it shows resistance to gentamicin, kanamycin, neomycin, streptomycin. Here, we report genome sequence of N. strain ZLM-10(T), which has 4.8-Mb G+C content 45.67%.