A5100733002- PI3K/AKT/mTOR signaling in cancer
- Gene expression and cancer classification
- Molecular Biology Techniques and Applications
- Bioinformatics and Genomic Networks
- Renal and related cancers
- RNA modifications and cancer
- Cancer Genomics and Diagnostics
- Nitrogen and Sulfur Effects on Brassica
- Lipid metabolism and biosynthesis
- Plant Molecular Biology Research
- Peroxisome Proliferator-Activated Receptors
- CRISPR and Genetic Engineering
- Genetics, Bioinformatics, and Biomedical Research
- Pluripotent Stem Cells Research
- MicroRNA in disease regulation
- Protein Degradation and Inhibitors
- Machine Learning in Bioinformatics
- Cell death mechanisms and regulation
- Photosynthetic Processes and Mechanisms
- Ziziphus Jujuba Studies and Applications
- Fibroblast Growth Factor Research
- Adipose Tissue and Metabolism
- RNA and protein synthesis mechanisms
- Ubiquitin and proteasome pathways
- Mitochondrial Function and Pathology
Jiangxi Academy of Agricultural Sciences
2012-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2023-2025
Beijing University of Chinese Medicine
2014-2024
Huazhong University of Science and Technology
2021-2024
Tongji Hospital
2021-2024
Second Affiliated Hospital of Zhejiang University
2024
China Medical University
2022-2024
Jiangxi Agricultural University
2024
Chongqing University
2023
First Affiliated Hospital of Hebei Medical University
2023
Abstract Autoinducer-2 (AI-2) is a quorum sensing signal that mediates communication within and between many bacterial species. However, its known receptors (LuxP LsrB families) are not found in all the bacteria capable of responding to this signaling molecule. Here, we identify third type AI-2 receptor, consisting dCACHE domain. binds domain chemoreceptors PctA TlpQ Pseudomonas aeruginosa , thus inducing chemotaxis biofilm formation. Boron-free preferred ligand for TlpQ. also domains...
Phosphatidylcholine (PC) is synthesized through the Kennedy pathway, but more than 50% of PC remodeled Lands cycle, i.e. deacylation and reacylation to attain final proper fatty acids within PC. The step catalyzed by lysophosphatidylcholine acyltransferase (LPCAT), we report here identification a novel LPCAT, which named LPCAT3. LPCAT3 belongs membrane-bound O-acyltransferase (MBOAT) family encodes protein 487 amino with calculated molecular mass 56 kDa. Membranes from HEK293 cells...
Personalized therapy provides the best outcome of cancer care and its implementation in clinic has been greatly facilitated by recent convergence enormous progress basic research, rapid advancement new tumor profiling technologies, an expanding compendium targeted therapeutics.
AGPAT6 is a member of the 1-acylglycerol-3-phosphate O-acyltransferase (AGPAT) family that appears to be important in triglyceride biosynthesis several tissues, but precise biochemical function enzyme unknown. In current study, we show microsomal glycerol-3-phosphate acyltransferase (GPAT). Membranes from HEK293 cells overexpressing human had higher levels GPAT activity. Substrate specificity studies suggested was active against both saturated and unsaturated long-chain fatty acyl-CoAs. Both...
Abstract Intrauterine adhesion (IUA) is characterized by endometrial fibrosis. S100A8/A9 plays an important role in inflammation and fibroblast activation. However, the of IUA remains unclear. In this study, we collect normal endometrium to verify expression S100A8/A9. Human stromal cells (hEnSCs) are isolated evaluate fibrosis progression after treatment. A porcine model established electrocautery injury confirm therapeutic effect menstrual blood-derived (MenSCs) on IUA. Our study reveals...
Background: Mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) are important mediators of intercellular communication and participate in numerous physiological pathological processes the body. This study aims to introduce research status, analyze hotspots, predict development trend through bibliometric analysis MSC-EVs. Methods: We searched all relevant literature on MSC-EVs from 2009 2021 Web Science. R-bibliometrix, VOSviewer, CiteSpace software were used visualize quantitative...
Leaf is the main photosynthetic organ at seedling stage of rapeseed and leaf size a crucial agronomic trait affecting yield. Understanding genetic mechanisms underlying therefore important for breeding. In this study, QTL mapping three traits related to size, i.e., width (LW), length (LL) area (LA), was performed using recombinant inbred line (RIL) population four QTLs LW, two LL LA were detected. Transcriptome analysis revealed that differentially expressed genes (DEGs) enriched in GO terms...
p38 mitogen-activated protein kinase (MAPK) is rapidly activated by stresses and believed to play an important role in the stress response. While Chk1 known mediate G2 DNA damage checkpoint control, was also reported have essential function this control. Here, we investigated further roles of cancer cells. We find that although activation strongly induced damage, its activity not required for checkpoint. In contrast, responsible execution control p53-deficient The inhibition has no effect on...
Abstract Background NCI60 cell lines are derived from cancers of 9 tissue origins and have been invaluable in vitro models for cancer research anti-cancer drug screen. Although extensive studies carried out to assess the molecular features related their sensitivities more than 100,000 chemical compounds, it remains unclear if how well these represent or model tumor tissues origin. Identification confirmation correct critical usage as systems translate into clinical potentials. Here we report...
Melanoma is the most malignant form of skin cancer and associated with a very poor prognosis. The aim this study was to evaluate apoptotic effects cudraflavone C on A375.S2 melanoma cells determine underlying mechanisms involved in apoptosis. Cell viability determined using MTT real-time cytotoxicity assays. Flow cytometric evaluation apoptosis performed after staining Annexin V-FITC propidium iodide. mitochondrial membrane potential evaluated JC-1 assay. Cellular ROS production measured...
Many bacteria secrete siderophores to enhance iron uptake under iron-restricted conditions. In this study, we found that Cupriavidus necator JMP134, a well-known aromatic pollutant-degrading bacterium, produces an unknown carboxylate-type siderophore named cupriabactin overcome limitation. Using genome mining, targeted mutagenesis, and biochemical analysis, discovered operon containing six open reading frames (cubA-F) in the C. JMP134 encodes proteins required for biosynthesis of...
Silique number is the most important component of yield in rapeseed (Brassica napus L.). To dissect mechanism underlying natural variation silique germplasm, a series studies were performed. A panel 331 core lines was employed to genome-wide association study (GWAS), and 27 loci (including 20 novel loci) identified. The difference between more- fewer-silique can be attributed accumulative differences flower setting rate. Each them accounted for 75.2% 24.8%, respectively. highly associated...
Abstract Differentiation of human embryonic stem cells (hESCs) into cells‐derived parathyroid‐like (hESC‐PT) has clinical significance in providing new therapies for congenital and acquired parathyroid insufficiency conditions. However, a highly reproducible, well‐documented method differentiation remains unavailable. By imitating the natural process development, we proposed hypothesis about vitro cells. Transcriptome, marker protein detection hormone (PTH) secretion assays were performed...
Peroxisomes are the exclusive site for beta-oxidation of very-long-chain fatty acids more than 20 carbons in length (VLCFAs). Although bulk dietary long-chain oxidized mitochondria, VLCFAs cannot be catabolized mitochondria and must shortened first by peroxisomal beta-oxidation. The regulation peroxisomal, mitochondrial, microsomal acid oxidation systems liver is mediated principally peroxisome proliferator-activated receptor alpha (PPARalpha). In this study we provide evidence that X (LXR)...
Numerous studies have used microarrays to identify gene signatures for predicting cancer patient clinical outcome and responses chemotherapy. However, the potential impact of expression profiling in diagnosis, prognosis development personalized treatment may not be fully exploited due lack consensus poor understanding underlying molecular mechanisms. We developed a novel approach derive breast context known biological pathways. Using unsupervised methods, patients were separated into...
Marked species-specific responses to agonists of the peroxisome proliferator-activated alpha receptor (PPAR alpha) have been observed in rats and dogs, two species typically used assess potential human risk pharmaceuticals development. In this study, we primary cultured rat dog hepatocytes investigate underlying mechanisms a novel PPAR -gamma coagonist, LY465608, relative fenofibrate, prototypical agonist. As expected, incubated with these demonstrated an increase number as evaluated by...
In traditional Chinese medicine, astragalus injection is used to treat diabetic nephropathy (DN). The current study was conducted determine the effects of on DN by assessing potential modulation transforming growth factor beta TGFβ/Smad signaling pathway. Diabetic, male KKAy mice, aged 14 weeks were randomly divided into a model group and an treatment group, while age-matched C57BL/6J mice selected as controls. received daily intraperitoneal injections (0.03 ml/10 g.d), equivalent volume...
Cohesins establish sister chromatid cohesion during S phase and are removed when cohesin Scc1 is cleaved by separase at anaphase onset. During this process, Smc3 undergoes a cycle of acetylation: acetylation Eco1 in stabilizes association with chromosomes, its deacetylation Hos1 allows re-use the next cell cycle. Here we find that has more immediate effect early budding yeast. depletion significantly delayed separation segregation. facilitated removal cohesins from chromosomes without...