A5047253694- Folate and B Vitamins Research
- Esophageal and GI Pathology
- Metabolism and Genetic Disorders
- Aldose Reductase and Taurine
- Prenatal Substance Exposure Effects
- Global Health and Surgery
- Iron Metabolism and Disorders
- Global Public Health Policies and Epidemiology
- Primary Care and Health Outcomes
- Diet and metabolism studies
- Trace Elements in Health
- HIV-related health complications and treatments
- Cerebral Palsy and Movement Disorders
- Telemedicine and Telehealth Implementation
- Acute Lymphoblastic Leukemia research
- Stroke Rehabilitation and Recovery
- Biomedical Ethics and Regulation
- Colorectal Cancer Screening and Detection
- Chronic Disease Management Strategies
- Epigenetics and DNA Methylation
- Case Reports on Hematomas
- Acute Kidney Injury Research
- Bariatric Surgery and Outcomes
- Congenital Anomalies and Fetal Surgery
- Focus Groups and Qualitative Methods
Dartmouth College
2012-2025
Dartmouth Institute for Health Policy and Clinical Practice
2018
University of Bradford
2005-2008
Tufts University
1997-2005
Jean Mayer Human Nutrition Research Center on Aging
1997-2004
Tufts Medical Center
2003
Rhode Island Hospital
2000-2002
United States Department of Agriculture
1998-2002
University of Verona
2002
Inserm
2000
DNA methylation, an essential epigenetic feature of that modulates gene expression and genomic integrity, is catalyzed by methyltransferases use the universal methyl donor S -adenosyl- l -methionine. Methylenetetrahydrofolate reductase (MTHFR) catalyzes synthesis 5-methyltetrahydrofolate (5-methylTHF), for methionine from homocysteine precursor In present study we sought to determine effect folate status on methylation with emphasis interaction common C677T mutation in MTHFR gene. A liquid...
A common mutation (C677T) in the gene encoding for methylenetetrahydrofolate reductase (MTHFR) (5-methyltetrahydrofolate:(acceptor) oxidoreductase, EC 1.7.99.5 ), a key regulatory enzyme one-carbon metabolism, results thermolabile variant of MTHFR with reduced activity vitro . In present study we used chromatographic method folate analysis to test hypothesis that this would be associated altered distribution red blood cell (RBC) folates. An alteration was found as manifested by presence...
Naturally occurring folates exist in multiple forms, differing pteridine ring structure and number of glutamate residues. The ability to measure these folate coenzymes tissues cells gives important information about vivo metabolism.Folates were heat-extracted from biological samples. A two-column HPLC system with four-channel coulometric electrochemical detection was used for analysis. An affinity column first purify the extract. Purified eluted onto a phenyl analytical column, utilizing...
Chronic alcoholism in humans is associated with the development of hyperhomocysteinemia, mechanism which remains unclear. Among causes hyperhomocysteinemia depletion folate, vitamin B12, or B6. Population-based studies indicate that folate strongest determinant and, most settings, supplementation effectively lowers elevated homocysteine levels. However, it not clear whether deficiency cause alcohol-related hyperhomocysteinemia.In present study, 10 male Sprague Dawley rats were fed...
Derangements of one-carbon metabolism can directly affect the integrity genome by producing inappropriate uracil insertion into DNA and altering patterns methylation. Vitamin B-12, a nutrient, serves as cofactor in synthesis precursors biological methylation nucleotide synthesis. We therefore examined whether vitamin B-12 deficiency induce these molecular anomalies colonic mucosa rats. Weanling male Sprague-Dawley rats (n = 30) were divided 2 groups fed either B-12–deficient diet or similar...
ABSTRACT Medical technology in gastroenterology spans devices, point-of-care diagnostics, digital health and artificial intelligence. We conducted a narrative review of US Food Drug Administration-reviewed medical on the available evidence base to support uptake clinical practice. 713 new GI devices were FDA-approved or cleared between 2013 2023. The most frequent technologies had indications for use general endoscopy (442 62.0% all technologies) with advanced being second common (172...
Background —The hyperhomocysteinemia regularly found in hemodialysis patients is largely refractory to combined oral B-vitamin supplementation featuring supraphysiological doses of folic acid. We evaluated whether a high-dose l -5-methyltetrahydrofolate–based regimen provided improved total homocysteine (tHcy)–lowering efficacy chronic patients. Methods and Results —We block-randomized 50 chronic, stable on the basis their screening predialysis tHcy levels, sex, dialysis center into 2 groups...
Abstract Patients with rheumatoid arthritis have subnormal vitamin B6 status, both quantitatively and functionally. Abnormal status in has been associated spontaneous tumor necrosis factor (TNF)-α production markers of inflammation, including C-reactive protein erythrocyte sedimentation rate. Impaired could be a result these patients may higher demand for B6. The aim this study was to determine if daily supplementation 50 mg pyridoxine 30 days can correct the static and/or functional...
Conflicting reports in the literature of appropriate assay procedures for measurement cysteine dioxygenase activity led us to evaluate procedure rat liver preparations. Cysteine was largely soluble fraction and stimulated by addition NAD+ Fe2+. The pH optimum enzyme 6.1. Addition an inhibitor pyridoxal 5-phosphate-dependent enzymes necessary prevent rapid removal reaction product sulfinate. sulfinate cysteic acid were separated anion-exchange HPLC on a polymer-based column with...
Abstract Background Dietary factors such as folate, vitamin B12, protein, and methionine are important for the excretion of arsenic via one-carbon metabolism in undernourished populations exposed to high levels drinking water. However, effects dietary on toenail concentrations well-nourished relatively low water unknown. Methods As part a population-based case–control study skin bladder cancer from USA, we evaluated relationships between consumption clippings. Consumption each factor was...
The catabolism of cysteine and cysteinesulfinate, the activities key enzymes in catabolic pathways, effects inhibitors specific on were investigated hepatocytes isolated from rats fed low (100 g casein/kg diet), moderate (300 diet) or high (600 levels dietary protein. Cysteine was catabolized predominantly by cysteinesulfinate-dependent pathways. dioxygenase activity increased with increases casein level, higher enzyme paralleled a greater total catabolite production (taurine + hypotaurine...