A5001437241- Plant Virus Research Studies
- Bacteriophages and microbial interactions
- Virology and Viral Diseases
- HIV Research and Treatment
- Trypanosoma species research and implications
- Monoclonal and Polyclonal Antibodies Research
- Animal Virus Infections Studies
- Viral Infections and Immunology Research
- Research on Leishmaniasis Studies
- Viral gastroenteritis research and epidemiology
- Insect Resistance and Genetics
- Protein Structure and Dynamics
- RNA and protein synthesis mechanisms
- Electrospun Nanofibers in Biomedical Applications
- HIV/AIDS drug development and treatment
- Viral Infections and Outbreaks Research
- Viral Infections and Vectors
- Carbon Nanotubes in Composites
- Lipid Membrane Structure and Behavior
- Insect and Pesticide Research
- Galectins and Cancer Biology
- thermodynamics and calorimetric analyses
- Synthesis and Biological Evaluation
- Bone Tissue Engineering Materials
- Immunotherapy and Immune Responses
University of the Basque Country
2015-2021
Instituto Biofisika
2016-2021
Fundación Biofísica Bizkaia
2010-2016
Instituto di Biofisica
2015
Triatoma virus (TrV) is a member of the insect family Dicistroviridae and consists small, non-enveloped capsid that encloses its positive-sense ssRNA genome. Using cryo-transmission electron microscopy three-dimensional reconstruction techniques combined with fitting available crystallographic models, this study analysed capsids corresponding to mature several RNA-empty TrV particles. After genome release, resulting empty displayed no prominent conformational changes respect full virion...
ABSTRACT In naked viruses, membrane breaching is a key step that must be performed for genome transfer into the target cells. Despite its importance, mechanisms behind this process remain poorly understood. The small protein VP4, encoded by genomes of most viruses order Picornavirales , has been shown to involved in alterations. Here we analyzed permeabilization activity natively nonmyristoylated VP4 from triatoma virus (TrV), belonging Dicistroviridae family within order. was produced as...
The contribution of membrane interfacial interactions to recognition membrane-embedded antigens by antibodies is currently unclear. This report demonstrates the optimization this type via chemical modification regions near but not directly involved in epitope. Using HIV-1 antibody 10E8 as a model, linear and polycyclic synthetic aromatic compounds are introduced at selected sites. Molecular dynamics simulations predict favorable these with viral lipid membrane, where epitope glycoprotein Env...
In viruses, uncoating and RNA release are two key steps of successfully infecting a target cell. During these steps, the capsid must undergo necessary conformational changes to allow egress. Despite their importance, processes poorly understood in family Dicistroviridae. Here, we used X-ray crystallography solve atomic structure Triatoma virus(TrV) empty particle (Protein Data Bank ID 5L7O), which is resulting after release. It observed that overall shape three individual proteins maintained...
In contrast to the current wealth of structural information concerning dicistrovirus particle structure, very little is known about their morphogenetic pathways. Here, we describe expression two ORFs encoded by Triatoma virus (TrV) genome. TrV, a member Cripavirus genus Dicistroviridae family, infects blood-sucking insects belonging Triatominae subfamily that act as vectors for transmission Trypanosoma cruzi, aetiological agent Chagas disease. We have established baculovirus-based model NS...
Chagas disease is caused by Trypanosoma cruzi, and humans acquire the parasite exposure to contaminated feces from hematophagous insect vectors known as triatomines. Triatoma virus (TrV) sole viral pathogen of triatomines, transmitted among insects through fecal-oral route and, it happens with T. infected release when defecating during or after blood uptake. In this work, we analysed occurrence anti-TrV antibodies in human sera endemic non-endemic countries, developed a mathematical model...
Future progress in materials for tissue engineering and 3D cell cultures applications requires control of two key physical properties: nanoscale mechanical properties mass transport. These requirements remain uncontrolled partly due to a lack parameters quantitative measurements. Using chitosan scaffolds as model system close-to-physiological conditions combination experimental techniques theory, we link structure with local nanomechanical properties. Additionally introduce parameter, the...
In viruses, uncoating and RNA release are two key steps of successfully infecting a target cell. During these steps, the capsid must undergo necessary conformational changes to allow egress. Despite their importance, processes poorly understood in family Dicistroviridae. Here, we used X-ray crystallography solve atomic structure Triatoma virus(TrV) empty particle (Protein Data Bank ID 5L7O), which is resulting after release. It observed that overall shape three individual proteins maintained...
Broadly neutralizing antibodies (bnAbs) against HIV-1 are frequently associated with the presence of autoreactivity/polyreactivity, a property that can limit their use as therapeutic agents. The bnAb 4E10, targeting conserved Membrane proximal external region (MPER) HIV-1, displays almost pan-neutralizing activity across globally circulating strains but exhibits nonspecific off-target interactions lipid membranes. hydrophobic apex third complementarity-determining heavy chain (CDRH3) loop,...