A5086218210- HIV Research and Treatment
- Monoclonal and Polyclonal Antibodies Research
- Lipid Membrane Structure and Behavior
- RNA Interference and Gene Delivery
- Immunotherapy and Immune Responses
- Chemistry and Chemical Engineering
- Antimicrobial agents and applications
- Diamond and Carbon-based Materials Research
- Metal and Thin Film Mechanics
- Photopolymerization techniques and applications
- Boron and Carbon Nanomaterials Research
- Advanced biosensing and bioanalysis techniques
- Glycosylation and Glycoproteins Research
- Immune Cell Function and Interaction
- Phenothiazines and Benzothiazines Synthesis and Activities
- Immunodeficiency and Autoimmune Disorders
- Thallium and Germanium Studies
- Advanced Electron Microscopy Techniques and Applications
- Advanced Fluorescence Microscopy Techniques
- Protein Structure and Dynamics
- Cytomegalovirus and herpesvirus research
- RNA Research and Splicing
- HIV/AIDS drug development and treatment
- Cell Image Analysis Techniques
- Galectins and Cancer Biology
Fundación Biofísica Bizkaia
2021-2025
University of the Basque Country
2017-2025
Instituto Biofisika
2017-2025
Consejo Superior de Investigaciones Científicas
2017
Antibodies against the Membrane-Proximal External Region (MPER) of Env gp41 subunit neutralize HIV-1 with exceptional breadth and potency. Due to lack knowledge on MPER native structure accessibility, different exclusive models have been proposed for molecular mechanism recognition by broadly neutralizing antibodies. Here, accessibility antibodies single virions has addressed through STED microscopy. imaging fluorescently labeled Fabs reveals a common pattern targeting or surface gp120. In...
Surfaces loaded with microbes can contribute to the propagation of a wide variety pathogens. In this work, antimicrobial potential hard coating (CrN) doped different proportions biocide element (copper) has been studied. The coatings have deposited by reactive high power impulse magnetron sputtering (HiPIMS) technology, on stainless steel and glass, materials used profusely in human traffic environments. microstructural mechanical properties evaluated, their antiviral (VSV-G) antibacterial...
The membrane-proximal external region (MPER), located in the carboxy-terminal section of HIV's envelope glycoprotein (Env) ectodomain, which is essential for viral entry into host cells, has gained considerable attention as a target HIV vaccine development due to exceptional neutralization breadth antibodies against MPER epitopes. A distinctive feature broadly neutralizing (bnAbs) targeting their requirement accommodate membrane surface antigen-binding fragment, or Fab moiety, optimize...
The 10E8 antibody targets a helical epitope in the membrane-proximal external region (MPER) and transmembrane domain (TMD) of envelope glycoprotein (Env) subunit gp41 is among broadest known neutralizing antibodies against HIV-1. Accordingly, this its mechanism action valuably inform design effective vaccines immunotherapies. exhibits unusual adaptations to attain specific, high-affinity binding MPER at viral membrane interface. Reversing charge basic paratope surface (from net positive...
Significance The high genetic diversity of HIV-1 continues to be a major barrier the development therapeutics for prevention and treatment. Here, we describe design an antibody platform that allows assembly highly avid, multispecific molecule targets, simultaneously, most conserved epitopes on envelope glycoprotein. combined multivalency multispecificity translates into extraordinary neutralization potency pan-neutralization strains, surpassing potent anti-HIV broadly neutralizing cocktails.
HIV‐1 antibodies targeting the carboxy‐terminal area of membrane‐proximal external region (ctMPER) are close to exerting viral pan‐neutralization. Here, we reconstituted ctMPER epitope as N‐terminal extremity Env glycoprotein transmembrane domain helix and immobilized it onto biosensor‐supported lipid bilayers. We assessed binding mechanism anti‐MPER antibody 10E8 through Surface Plasmon Resonance, found, equilibrium kinetic analyses a function bilayer thickness, peptide length, paratope...
Preventing microbiological surface contamination in public spaces is nowadays of high priority. The proliferation a microbial infection may arise through air, water, or direct contact with infected surfaces. Chemical sanitization one the most effective approaches to avoid microorganisms. However, extended chemicals for cleaning purposes such as chlorine, hydrogen peroxide ethanol lead long-term diseases well drowsiness respiratory issues, not mention environmental issues associated their...
The contribution of membrane interfacial interactions to recognition membrane-embedded antigens by antibodies is currently unclear. This report demonstrates the optimization this type via chemical modification regions near but not directly involved in epitope. Using HIV-1 antibody 10E8 as a model, linear and polycyclic synthetic aromatic compounds are introduced at selected sites. Molecular dynamics simulations predict favorable these with viral lipid membrane, where epitope glycoprotein Env...
Establishment of interactions with the envelope lipids is a cardinal feature broadly neutralizing antibodies (bnAbs) that recognize Env membrane-proximal external region (MPER) HIV. The lipid constitutes relevant component full "quinary" MPER epitope, and thus may be optimized through engineering their capacity to interact lipids. However, role chemically complex nanoenvironment in mechanism molecular recognition viral neutralization remains poorly understood. To approach this issue, we...
The 4E10 antibody displays an extreme breadth of HIV-1 neutralization and therefore constitutes a suitable model system for structure-guided vaccine design immunotherapeutics against AIDS. In this regard, the relevance autoreactivity with membrane lipids biological function is still subject controversy. To address dispute, herein we have compared partitioning ability several its variants, which were mutated at region paratope surface in contact interface. We first employed physical...
The envelope glycoprotein (Env) enables HIV-1 cell entry through fusion of host-cell and viral membranes induced by the transmembrane subunit gp41. Antibodies targeting C-terminal sequence membrane-proximal external region (C-MPER) block fusogenic activity gp41 achieve neutralization divergent strains isolates. Thus, recreating structure that generates broadly neutralizing C-MPER antibodies during infection is a major goal in HIV vaccine development. Here, we have reconstituted peptide...
Abstract Antibodies against the carboxy-terminal section of membrane-proximal external region (C-MPER) HIV-1 envelope glycoprotein (Env) are considered as nearly pan-neutralizing. Development vaccines capable producing analogous broadly neutralizing antibodies requires deep understanding mechanism that underlies C-MPER recognition in membranes. Here, we use archetypic 10E8 antibody and a variety biophysical techniques including single-molecule approaches to study molecular membrane mimetics....
Broadly neutralizing antibodies (bnAbs) against HIV-1 are frequently associated with the presence of autoreactivity/polyreactivity, a property that can limit their use as therapeutic agents. The bnAb 4E10, targeting conserved Membrane proximal external region (MPER) HIV-1, displays almost pan-neutralizing activity across globally circulating strains but exhibits nonspecific off-target interactions lipid membranes. hydrophobic apex third complementarity-determining heavy chain (CDRH3) loop,...
Antibody engagement with the membrane-proximal external region (MPER) of envelope glycoprotein (Env) HIV-1 constitutes a distinctive molecular recognition phenomenon, full appreciation which is crucial for understanding mechanisms that underlie broad neutralization virus. Recognition Env antigen seems to depend on two specific features developed by antibodies MPER specificity: (i) large cavity at antigen-binding site holds epitope amphipathic helix; and (ii) membrane-accommodating Fab...
Preventing microbiological surface contamination in public spaces is nowadays of high priority. The proliferation a viral or bacterial infection may arise through air, water, direct contact with infected surfaces. Chemical sanitization one the most effective approaches to avoid microorganisms. However, extended chemicals for cleaning purposes such as chlorine, hydrogen peroxide ethanol lead long-term diseases well drowsiness respiratory issues, not mention environmental issues associated...
Preventing microbiological surface contamination in public spaces is nowadays of high priority. The proliferation a viral or bacterial infection may arise through air, water, direct contact with infected surfaces. Chemical sanitization one the most effective approaches to avoid microorganisms. However, extended chemicals for cleaning purposes such as chlorine, hydrogen peroxide ethanol lead long-term diseases well drowsiness respiratory issues, not mention environmental issues associated...
Terapia antirretrobiral konbinatuaren erabilera iraultza bat izan zen GIBaren infekzioaren tratamenduan eta prebentzioan. Hala ere, terapia hau ez da gai birusa organismotik erabat ezabatzeko, GIB infekzio kasu berrien munduko tasak mantso jaisten jarraitu du. Beraz, prebentzio tratamendurako ikuspegi berriak aztertzeko beharra dago. Antigorputzak aurkako aukera terapeutiko gisa proposatuak dira, epitopo biralak blokeatzeaz gain ostalariaren immunitate sistemarekin elkar ekiteko gaitasuna...
Direct contact with a contaminated surface or cross contamination between surfaces can contribute to the propagation of wide variety pathogens. The use disinfectants often rises concerns due presence hazardous chemicals, ineffectiveness against specific pathogens induced degradation materials.In this work, disinfection potential hard coating (CrN) doped different proportions biocide element (copper) has been studied. coatings have deposited by reactive high power impulse magnetron sputtering...
Direct contact with a contaminated surface or cross contamination between surfaces can contribute to the propagation of wide variety pathogens. The use disinfectants often rises concerns due presence hazardous chemicals, ineffectiveness against specific pathogens induced degradation materials.In this work, disinfection potential hard coating (CrN) doped different proportions biocide element (copper) has been studied. coatings have deposited by reactive high power impulse magnetron sputtering...