A5030094029- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Crystallography and molecular interactions
- Force Microscopy Techniques and Applications
- Molecular Sensors and Ion Detection
- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- Cancer therapeutics and mechanisms
- Orbital Angular Momentum in Optics
- Cellular Mechanics and Interactions
- Nanoparticle-Based Drug Delivery
- RNA Interference and Gene Delivery
- Trace Elements in Health
- Curcumin's Biomedical Applications
- Microplastics and Plastic Pollution
- Electrochemical Analysis and Applications
- Mechanical and Optical Resonators
- Microfluidic and Bio-sensing Technologies
- Thin-Film Transistor Technologies
- Luminescence and Fluorescent Materials
- Organic Electronics and Photovoltaics
- Silicon Nanostructures and Photoluminescence
- Conducting polymers and applications
- Phytochemistry and Biological Activities
- Neuroscience and Neuropharmacology Research
Wuhan Institute of Technology
2021-2025
Northeast Agricultural University
2024
Shenzhen Institutes of Advanced Technology
2024
Chinese Academy of Sciences
2024
Howard Hughes Medical Institute
2018-2024
Cornell University
2018-2024
China Academy of Space Technology
2024
Chongqing Medical University
2024
Northwestern Polytechnical University
2024
Fujian Medical University
2024
The UV- and sensor-induced interferences to living systems pose a barrier for in vivo Zn2+ imaging. In this work, an intramolecular charge transfer (ICT) fluorophore of smaller aromatic plane, 4-amino-7-nitro-2,1,3-benzoxadiazole, was adopted construct visible light excited fluorescent sensor, NBD-TPEA. This sensor demonstrates ICT absorption band, large Stokes shift, biocompatibility. It emits weakly (Φ = 0.003) without pH dependence at 7.1−10.1, the λex λem are 469 (ε469 2.1 × 104 M−1...
Designing synthetic protein hydrogels with tailored mechanical properties similar to naturally occurring tissues is an eternal pursuit in tissue engineering and stem cell cancer research. However, it remains challenging correlate the of nanomechanics individual building blocks. Here we use single-molecule force spectroscopy, theoretical modeling prove that are predictable based on hierarchy cross-linkers load-bearing modules at molecular level. These findings provide a framework for...
A ratiometric fluorescent Cd2+ sensor DBITA which featured the Cd2+-induced red-shift of emission (53 nm) and picomolar sensitivity in both aqueous media living cells was developed.
A fluorescent Zn(2+) sensor based on the 2-(2'-pyridinyl)benzoimidazole (2-PBI) fluorophore has been devised by incorporation with a ionophore, bis(pyridin-2-ylmethyl)amine. The (PBITA) demonstrates Zn(2+)-specific emission shift and enhancement 1:1 binding ratio. Due to Zn(2+)-induced coplanation of 2-PBI via reversion/rotation, PBITA is shown behave as ratiometric sensor. intracellular imaging ability tested in HeLa cells using confocal microscope.
Abstract: Systemic administration of chemotherapy for cancer often has toxic side effects, limiting the doses that can be used in its treatment. In this study, we developed methoxy poly(ethylene glycol)-poly(caprolactone) (MPEG-PCL) micelles loaded with curcumin and doxorubicin (Cur-Dox/MPEG-PCL) were tolerated by recipient mice had enhanced antitumor effects fewer effects. It was shown these Cur-Dox/MPEG-PCL could release slowly vitro. The long circulation time MPEG-PCL slow rate vivo may...
Abstract: Radiation pneumonitis (RP) is an important dose-limiting toxicity during thoracic radiotherapy. Previous investigations have shown that curcumin used for the treatment of inflammatory conditions and cancer, suggesting may prevent RP sensitize cancer cells to irradiation. However, clinical advancement limited by its poor water solubility low bioavailability after oral administration. Here, a water-soluble liposomal system was developed investigate prevention sensitizing effects...
DNA torsional elastic properties play a crucial role in structure, topology, and the regulation of motor protein progression. However, direct measurements these parameters are experimentally challenging. Here, we present constant-extension method integrated into an angular optical trap to directly measure torque during supercoiling. We measured twist persistence length extended be 22 nm under extremely low force (∼0.02 pN) plectonemic 24 nm. In addition, implemented rigorous data analysis...
Different interacting signaling modules involving Ca(2+)/calmodulin-dependent myosin light chain kinase, Ca(2+)-independent regulatory phosphorylation, phosphatase inhibition, and actin filament-based proteins are proposed as specific cellular mechanisms involved in the regulation of smooth muscle contraction. However, relative importance is not well defined. By using tamoxifen-activated muscle-specific knock-out kinase mice, we analyzed its role tonic airway Knock-out both tracheal...
A newly developed fluorescent sensor, Naph-BPEA, shows a specific turn-on response to Zn2+ and can be excited by visible light. The in situ nuclear imaging HeLa HepG2 cells reveals the envelope penetrability of sensor. sensor location zebrafish larva was also demonstrated.
Significance RNA polymerase (RNAP) carries out transcription from DNA to RNA. The double-stranded helical nature of necessitates RNAP rotation during active elongation, leading supercoiling and the accumulation torsional stress, which may ultimately stall transcription. As a motor, generates works against torsion, but it remains unclear whether how RNAP’s torque generation capacity be regulated. In this work, real-time single-molecule transcriptional assays revealed that, in response...
Dexamethasone (DEX) has been demonstrated to inhibit the inflammatory corneal neovascularization (CNV). However, therapeutic efficacy of DEX is limited by poor bioavailability conventional eye drops and increased risk hormonal glaucoma cataract associated with prolonged frequent usage. To address these limitations, we have developed a novel DEX-loaded, reactive oxygen species (ROS)-responsive, controlled-release nanogel, termed DEX@INHANGs. This advanced nanogel system constructed formation...
A novel fluorescent chemosensor (L) demonstrates a remarkable selectivity and sensitivity for zinc(II) ion as evidenced from the solution characterisations in vitro experiments using Hela cell lines.
Abstract In this study, we designed a polyvinyl alcohol (PVA)‐alginate based hydrogel and evaluated its cytocompatibility printability. The samples were fabricated by 3D printing using freeze–thaw process. scanning electron microscope, material testing machine, rheometer, cell counting kit‐8 assay used to examine the morphology, mechanical properties, rheological cytocompatiblity of scaffolds, respectively. strength, cytocompatiblity, crosslinking time, printability remarkably improved with...
The angular optical trap (AOT) is a powerful instrument for measuring the torsional and rotational properties of biological molecule. Thus far, AOT studies DNA mechanics have been carried out using high numerical aperture oil-immersion objective, which permits strong trapping, but inevitably introduces spherical aberrations due to glass-aqueous interface. However, impact these on torque measurements not fully understood experimentally, partly lack theoretical guidance. Here, we present...