A5090921098- Bacterial Infections and Vaccines
- Reproductive tract infections research
- Syphilis Diagnosis and Treatment
- Pneumonia and Respiratory Infections
- Antibiotic Resistance in Bacteria
- Streptococcal Infections and Treatments
- Bacterial Genetics and Biotechnology
- Sex work and related issues
- Escherichia coli research studies
- Viral gastroenteritis research and epidemiology
- Microbial infections and disease research
- Vibrio bacteria research studies
- Salmonella and Campylobacter epidemiology
- Gut microbiota and health
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Trace Elements in Health
- RNA and protein synthesis mechanisms
- Urinary and Genital Oncology Studies
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Lipid Membrane Structure and Behavior
- Advanced Nanomaterials in Catalysis
- Antimicrobial Peptides and Activities
- Urological Disorders and Treatments
- Enzyme Production and Characterization
National Institute of Infectious Diseases
2016-2025
Gifu University
2017
National Institutes of Health
2006-2011
Sojo University
2000
ABSTRACT Recently, the first Neisseria gonorrhoeae strain (H041) that is highly resistant to extended-spectrum cephalosporin (ESC) ceftriaxone, last remaining option for empirical first-line treatment, was isolated. We performed a detailed characterization of H041, phenotypically and genetically, confirm finding, examine its antimicrobial resistance (AMR), elucidate mechanisms. H041 examined using seven species-confirmatory tests, antibiograms (30 antimicrobials), porB sequencing, N....
We have characterized in detail a new ceftriaxone- and multidrug-resistant Neisseria gonorrhoeae strain (FC428) isolated Japan 2015. FC428 differed from previous ceftriaxone-resistant strains contained novel mosaic penA allele encoding penicillin-binding protein 2 (PBP 2). However, the resistance-determining 3'-terminal region of was almost identical to regions two previously reported Australia Japan, indicating that both conserved ceftriaxone PBP might spread.
In 2009, the first high-level ceftriaxone-resistant Neisseria gonorrhoeae strain (H041) was isolated in Kyoto, Japan. The present study describes an intensified surveillance (antimicrobial resistance and molecular typing) of isolates Kyoto its neighboring prefecture Osaka, Japan, 2010 to 2012, which initiated after identification H041. From April March 193 N. were collected MICs (μg/ml) six antimicrobials, including ceftriaxone, determined. All showed susceptibility ceftriaxone cefixime (MIC...
ABSTRACT In Neisse ria gonorrhoeae , the mosaic type of penA which encodes penicillin-binding protein 2 (PBP 2), is associated with reduced susceptibility to oral cephalosporins. To investigate relatedness N. clinical isolates susceptibility, we sequenced genes 32 isolates. Five different amino acid sequence types PBP were identified, but all seemed be derivatives pattern X 2-X). However, multilocus typing showed that belonged six types. As 2-X was identified in three types, horizontal...
Neisseria gonorrhoeae strains with resistance to extended-spectrum cephalosporins (ESCs), last options for first-line monotherapy of gonorrhoea, likely emerged and initially disseminated in Japan, followed by international transmission. In recent years, multi-locus sequence typing (MLST) ST1901 N. multiantigen (NG-MAST) ST1407 isolates the mosaic penicillin-binding protein (PBP) 2 XXXIV have accounted most ESC globally. Our aim was elucidate initial emergence transmission ESC-resistant...
Objectives To detect microorganisms responsible for male acute urethritis and to define the microbiology of non‐gonococcal urethritis. Methods The present study comprised 424 men with symptoms signs compatible Their urethral swabs first‐voided urine underwent detection microorganisms. Demographic characteristics clinical features Mycoplasma genitalium ‐, Ureaplasma urealyticum Haemophilus influenza adenovirus‐ or Herpes simplex virus‐positive monomicrobial urethritis, all‐examined...
The first extensively drug resistant (XDR) Neisseria gonorrhoeae strain with high resistance to the extended-spectrum cephalosporin ceftriaxone was identified in 2009 Japan, but no other this antimicrobial-resistance profile has been reported since. However, surveillance date based on phenotypic methods and sequence typing, not genome sequencing. Therefore, little is known about local population structure at genomic level, how determinants lineages are distributed evolve. We analysed...
Ceftriaxone resistance in Neisseria gonorrhoeae is a major public health concern globally because high-dose (1 g) injection of ceftriaxone the only remaining option for empirical monotherapy gonorrhoea. The ceftriaxone-resistant gonococcal strain FC428, cultured Osaka 2015, suspected to have spread nationally and internationally. We describe complete finished genomes FC428 two closely related isolates from examine genomic epidemiology these plus three Hyogo 2016–17 four 2017 Australia,...
MDR and XDR Neisseria gonorrhoeae strains remain major public health concerns internationally, quality-assured global gonococcal antimicrobial resistance (AMR) surveillance is imperative. The WHO Gonococcal Antimicrobial Surveillance Programme (GASP) Enhanced GASP (EGASP), including metadata WGS, are expanding internationally. We present the phenotypic, genetic reference genome characteristics of 2024 (n = 15) for quality assurance worldwide. All superseded 14) were identically characterized.
Abstract Background Antimicrobial resistance in Neisseria gonorrhoeae is a global health concern. Strains from two internationally circulating sequence types, ST-7363 and ST-1901, have acquired to third-generation cephalosporins, mainly due mosaic penA alleles. These STs were first detected Japan; however, the timeline, mechanism, process of emergence spread these alleles other countries remain unknown. Methods We studied evolution by obtaining complete genomes three Japanese ST-1901...
Abstract Background Serratia marcescens is a gram-negative bacterium and often causes nosocomial infections. There have been few studies of the virulence factors this bacterium. The only S. hemolytic cytotoxic factor reported, thus far, hemolysin ShlA. Results An shl AB deletion mutant was constructed shown to no contact activity. However, retained activity on human blood agar plates, indicating presence another factor. Functional cloning identified phospholipase A (PhlA) with plates. phl...
Neisseria gonorrhoeae is a major public health problem globally, especially because the bacterium has developed resistance to most antimicrobials introduced for first-line treatment of gonorrhea. In present study, 96 N. isolates with high-level penicillin from 121 clinical in Thailand were examined investigate changes related their plasmid-mediated and molecular epidemiological relationships. A β-lactamase (TEM) gene variant, bla(TEM-135), that may be precursor transitional stage traditional...
ABSTRACT Mosaic penA alleles have caused most of the cephalosporin resistance in Neisseria gonorrhoeae , but their evolution is mostly unknown. The gene from cinerea strain AM1601 (ceftriaxone MIC, 1.0 μg/ml) ceftriaxone (MIC, 1 a ceftriaxone-susceptible gonococcal strain. 3′-terminal half was almost identical to that ceftriaxone-resistant GU140106 and FC428 strains. N. can serve as reservoir resistance-mediating sequences be transferred gonococci.
Abstract Objectives It has been suggested that the emergence of ceftriaxone-resistant strains Neisseria gonorrhoeae involves incorporation penA gene from commensal spp. are resistant to ceftriaxone. However, mechanism this mosaic generation is unknown. Methods We obtained 10 showing ceftriaxone MIC >0.5 mg/L. The similarity region these and some N. was investigated. To obtain transformants, spp., lactamica, gDNA used as donor DNA a strain recipient. Results sequence in certain regions...
Ten penicillinase-producing Neisseria gonorrhoeae (PPNG) strains isolated from 2000 to 2008 were characterized by multilocus sequence typing, multiantigen and plasmid typing. Sequence analysis showed that 8 contained a TEM-1 beta-lactamase gene. However, two other genetically distinct PPNG strains, in 2004 2008, each TEM-135 on different plasmids, Toronto/Rio type R an Asia plasmid, suggesting independent origins of these strains.
ABSTRACT The pathogenicity island termed locus of enterocyte effacement (LEE) encodes a type 3 protein secretion system, whose function is required for full virulence enterohemorrhagic Escherichia coli (EHEC). GrlR and GrlA are LEE-encoded negative positive regulators, respectively, controlling transcription the ler gene, which central activator LEE gene expression. We previously reported that GrlR-GrlA regulatory system controls not only genes but also flagellar expression in EHEC (S. Iyoda...
ObjectivesAntimicrobial resistance in Neisseria gonorrhoeae is a major public health concern globally. We report the first verified treatment failure of gonorrhoea with 2 g azithromycin (extended-release formulation) Japan and characteristics corresponding N. isolates.
The novel dual-target triazaacenaphthylene, gepotidacin, recently showed promising results in its Phase III randomized controlled trial for the treatment of gonorrhoea. We investigated alterations gepotidacin GyrA and ParC targets gonococci by silico mining publicly available global genomes (n = 33 213) determined MICs isolates with A92 combined other and/or alterations.
There have been few comprehensive studies on Haemophilus influenza-positive urethritis.In this retrospective study, we enrolled 68 men with H. influenzae-positive urethritis, including coinfections Neisseria gonorrhoeae, Chlamydia trachomatis, and/or genital mycoplasmas: 2, 3, 20, and 43 treated ceftriaxone, levofloxacin, sitafloxacin, extended-release azithromycin (azithromycin-SR), respectively. We assessed microbiological outcomes in 54 clinical 46 monomicrobial nongonococcal urethritis....
While invasive meningococcal disease (IMD) is a major public concern worldwide, IMD categorized as rare infectious in Japan and, thus, its causative agents and epidemiology have not yet been characterized detail. In the present study, we used molecular methods to epidemiologically characterize 291 strains isolated over 17-year period between 2003 2020 by whole genome sequencing (WGS). Serogroup Y meningococci (MenY) were most abundant, followed B (MenB) then C W among from patients, while...
Although we previously reported that some meningococcal isolates in Japan were resistant to penicillin (PCG) and ciprofloxacin (CIP), the antibiotic susceptibilities of