A5063667609- Bacterial Infections and Vaccines
- Reproductive tract infections research
- Parasitic Infections and Diagnostics
- Legionella and Acanthamoeba research
- Salmonella and Campylobacter epidemiology
- Amoebic Infections and Treatments
- Syphilis Diagnosis and Treatment
- Marine and fisheries research
- Viral gastroenteritis research and epidemiology
- Water Treatment and Disinfection
- Parasite Biology and Host Interactions
- Vibrio bacteria research studies
- Water Quality Monitoring Technologies
- Pneumonia and Respiratory Infections
- Turtle Biology and Conservation
- Aquaculture disease management and microbiota
- Bird parasitology and diseases
- Escherichia coli research studies
- Animal Virus Infections Studies
- Bacterial biofilms and quorum sensing
- Coastal and Marine Management
- Physiological and biochemical adaptations
- Bacteriophages and microbial interactions
- Wastewater Treatment and Nitrogen Removal
- Mycobacterium research and diagnosis
Okayama University of Science
2018-2024
Kanagawa Prefectural Institute of Public Health
2010-2021
National Institute of Infectious Diseases
1987-2017
Stanford University
2017
Toyohashi University of Technology
2014
Public Health Laboratory
1989-2003
Chelsea and Westminster Hospital
1997
Walker (United States)
1997
Shimizu (Japan)
1975
The University of Tokyo
1975
Abstract A serious disease of amphibians caused by the chytrid fungus Batrachochytrium dendrobatidis was first found in Japan December 2006 imported pet frogs. This report chytridiomycosis Asia. To assess risk pandemic to Japanese frogs, we surveyed distribution among captive and wild frog populations. We established a nested PCR assay that uses two pairs primers amplify internal transcribed spacer (ITS) region ribosomal RNA cassette detect mild fungal infections from as little 0.001 pg (1...
ABSTRACT In Neisse ria gonorrhoeae , the mosaic type of penA which encodes penicillin-binding protein 2 (PBP 2), is associated with reduced susceptibility to oral cephalosporins. To investigate relatedness N. clinical isolates susceptibility, we sequenced genes 32 isolates. Five different amino acid sequence types PBP were identified, but all seemed be derivatives pattern X 2-X). However, multilocus typing showed that belonged six types. As 2-X was identified in three types, horizontal...
Neisseria gonorrhoeae strains with resistance to extended-spectrum cephalosporins (ESCs), last options for first-line monotherapy of gonorrhoea, likely emerged and initially disseminated in Japan, followed by international transmission. In recent years, multi-locus sequence typing (MLST) ST1901 N. multiantigen (NG-MAST) ST1407 isolates the mosaic penicillin-binding protein (PBP) 2 XXXIV have accounted most ESC globally. Our aim was elucidate initial emergence transmission ESC-resistant...
The first extensively drug resistant (XDR) Neisseria gonorrhoeae strain with high resistance to the extended-spectrum cephalosporin ceftriaxone was identified in 2009 Japan, but no other this antimicrobial-resistance profile has been reported since. However, surveillance date based on phenotypic methods and sequence typing, not genome sequencing. Therefore, little is known about local population structure at genomic level, how determinants lineages are distributed evolve. We analysed...
This study determined the occurrence of legionellae in private houses for which there were no available data on aquatic environments other than water supply system. From June 2013 to November 2014, we collected 138 and 90 swab samples from 19 houses. Legionella DNA was detected via a loop-mediated isothermal amplification assay 66 (47·8%) 17 (18·9%) samples. High detection rates observed washing machines aquariums. spp. isolated 9 (6·5%) 3 (3·3%) pneumophila SG 1 outlet bathtub spout bath...
Abstract Background Antimicrobial resistance in Neisseria gonorrhoeae is a global health concern. Strains from two internationally circulating sequence types, ST-7363 and ST-1901, have acquired to third-generation cephalosporins, mainly due mosaic penA alleles. These STs were first detected Japan; however, the timeline, mechanism, process of emergence spread these alleles other countries remain unknown. Methods We studied evolution by obtaining complete genomes three Japanese ST-1901...
From November 2000 to July 2002, 112 fecal samples from pet reptiles, including 18 turtles, 71 lizards and 23 snakes, sold at a shop were examined for the prevalence of Salmonella spp. in Japan. isolated 83 (74.1%) samples, total isolates identified as subspecies I IV. The majority (62.5%) belonged 54 could be any 28 serovars. predominant serovars found S. Bardo, Newport Panama, which cause human salmonellosis. These results indicate that reptiles may potential infectious source salmonellosis
This is the first report of amphibian chytridiomycosis in Asia. We discovered a lethal outbreak Japan, among 45 exotic frogs from 18 species kept for breeding by private owner. Of these frogs, 16 died and another 7 were found to be infected chytrid fungus Batrachochytrium dendrobatidis (Bd) but survived after treatment. Bd was detected 9 (Lepidobatrachus laevis, Ceratophrys cornuta, C. cranwelli, ornata, calcarata, Chacophrys pierotti, Occidozyga lima, Leptodactylus pentadactylus...
Enterohemorrhagic Escherichia coli (EHEC) O157:H7 infection causes severe diseases such as bloody diarrhea and hemolytic uremic syndrome (HUS). Although EHEC strains have exhibited high genetic variability, their abilities to cause human not been fully examined.Clade typing stx subtyping of strains, which were isolated in Japan during 1999-2011 from 269 HUS patients 387 asymptomatic carriers (ACs) showed distinct pulsed-field gel electrophoresis patterns, performed determine relationships...
Analysis of 182 Neisseria meningitidis strains isolated over the past 30 years in Japan by serogroup typing and multilocus sequence (MLST) was performed. The serogroups Japanese isolates were B (103 isolates), Y (39), W135 (1) non-groupable (39). By MLST analysis, 65 different types (ST) identified, 42 which not found database as January 2004 seemed to be unique Japan. Statistical analysis results revealed that, although genetically divergent, they classified into six major clonal complexes...
This study was performed to determine the prevalence, antimicrobial susceptibility, and genetic relatedness of Salmonella enterica subsp. Campylobacter spp. in poultry meat, analyze association types these bacteria with their geographical distribution resistance profiles. isolates have been detected, respectively, 54 71 samples out 100 tested. Nine serotypes were found, including S. serovar Infantis (33%), Schwarzengrund (12%), Manhattan (9%), others. jejuni C. coli detected 64 (64%) 14...
In Japan, hot springs and public baths are the major sources of legionellosis. 2015, an outbreak Legionnaires' disease occurred among 7 patients who had visited a spa house. Laboratory investigation indicated that L. pneumophila serogroup 1 13 strains caused these were genetically related.
The present study was performed to investigate HIF-1alpha (hypoxia-inducible factor-1alpha) expression in a large number of immunohistochemically and ultrastructurally characterized surgically removed pituitary tumours. potential relation with outcome variables as well the presence tumours treated dopamine agonists octreotide, long-acting somatostatin analogue also investigated. immunoreactivity confined nucleoplasm whereas nucleoli were unconspicuous. distribution evident normal...
ABSTRACT Salmonella enterica serovar Typhimurium was isolated from the intestinal contents of Rattus rattus and norvegicus house rats captured at two buildings, designated buildings J YS, in Yokohama City, Japan. From October 1997 to September 1998, 52 339 (15.3%) were found carry definitive phage type 104 (DT104). In building J, 26 161 (16.1%) carried DT104 over 1-year study period, compared 178 (14.6%) YS. The isolation rates R. similar buildings. Most strains (24 26) showed resistance...
To evaluate the relationship between incidence of legionellosis and Legionella concentrations in bathwater, we sent a questionnaire to 76 prefectural municipal public health laboratories Japan found that 35 had encountered cases implemented investigations determine sources infections. Based on results questionnaire, were able analyze various characteristics patients, facilities thought be associated with cases, species serogroups isolates Legionella. Ninety-six included this study. The...
Abstract Salmonella enterica subsp. serovar Enteritidis ( S . Enteritidis) was responsible for a worldwide pandemic during the 1980s and 1990s; however, changes in dominant lineage before after this event remain unknown. This study determined lineages Japan using multilocus sequence typing (MLST). Thirty strains were collected between 1973 2004, consisting of 27 human from individual episodes, bovine strain, liquid egg strain an eggshell strain. Strains showed nine phage types 17...
An outbreak of diarrhea due to infection with Cryptosporidium occurred among the staff members and customers who visited one 10 public houses or a dancing school in building Hiratsuka, Kanagawa Prefecture, at end summer 1994. The epidemiological surveys by questionnaire revealed that 461 out 736 persons investigated complained cholera-like flu-like illness. clinical manifestations included mucous and/or watery (96.7%), abdominal pain (61.6%), fever (54.2%: lower than 39°C= 84.1%, higher...
We investigated whether raccoons (Procyon lotor) carried leptospires in their kidneys Japan. Leptospira was isolated from 2 of 71 captured Kanagawa Prefecture and 1 53 at a zoological park Nagasaki Prefecture. Anti-Leptospira antibodies were detected 16 124 (12.9%) 33 (62.3%) Nagasaki, respectively. The partial nucleotide sequences flaB genes suggested that the isolates belonged to L. interrogans. serovars identified as Copenhageni/Icterohaemorrhagiae (1 strain Kanagawa) Hebdomadis both...
An outbreak of enterohemorrhagic Escherichia coli (EHEC) O157:H7 infection occurred in October 2016 Kanagawa, Japan. A total 61 patients and 17 asymptomatic cases EHEC were confirmed by laboratory testing. Among them, 24 hospitalized 4 developed hemolytic-uremic syndrome. epidemiological investigation revealed that this was associated with the consumption uncooked minced meat cutlets sold frozen at branches a supermarket chain. The implicated made mixture beef, pork, onions, eggs. All 40...
In this paper, we examine 2 case reports for different reptile-related Salmonella enterica subspecies serotypes. 1, a 5-year-old boy presented with gastroenteritis caused by S. serovar Poona. The suspected source of infection was turtle kept at the patient's home. 2, 4-year-old Abony. Pulsed-field gel electrophoresis analysis suggested that tortoise home infection. This paper presents review literature and an examination cases regarding turtle-associated salmonellosis in Japan.