A5039093132- Diet and metabolism studies
- Adipose Tissue and Metabolism
- Mitochondrial Function and Pathology
- Nutrition and Health in Aging
- Muscle Physiology and Disorders
- Liver Disease Diagnosis and Treatment
- Bariatric Surgery and Outcomes
- Muscle metabolism and nutrition
- Metabolism, Diabetes, and Cancer
- Adipokines, Inflammation, and Metabolic Diseases
- ATP Synthase and ATPases Research
- Diet, Metabolism, and Disease
- GDF15 and Related Biomarkers
- Cancer, Hypoxia, and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Connective tissue disorders research
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Exercise and Physiological Responses
- Metabolism and Genetic Disorders
- Pancreatic function and diabetes
- Long-Term Effects of COVID-19
- Body Contouring and Surgery
- Circadian rhythm and melatonin
- Neurobiology and Insect Physiology Research
- Pulmonary Hypertension Research and Treatments
Cleveland Clinic
2019-2025
Pennington Biomedical Research Center
2018-2025
Louisiana State University
2020-2025
Baton Rouge Clinic
2018-2024
Cleveland Clinic Lerner College of Medicine
2018-2023
Cleveland State University
2016
Mitochondria exist as a morphologically plastic network driven by cellular bioenergetic demand. Induction of fusion and fission machinery allows the organelle to regulate quality control substrate flux. Physiological stressors promote fragmentation mitochondrial network, process implicated in onset metabolic disease, including type 2 diabetes obesity. It is well-known that exercise training improves skeletal muscle volume, number, density. However, effect on dynamics remains unclear.Ten...
Abstract Background Sarcopenic obesity is a highly prevalent disease with poor survival and ineffective medical interventions. Mitochondrial dysfunction purported to be central in the pathogenesis of sarcopenic by impairing both organelle biogenesis quality control. We have previously identified that mitochondrial‐targeted furazano[3,4‐b]pyrazine named BAM15 orally available selectively lowers respiratory coupling efficiency protects against diet‐induced mice. Here, we tested hypothesis...
Abstract Cancer cachexia is a common, debilitating condition with limited therapeutic options. Using an established mouse model of lung cancer, we find that characterized by reduced food intake, spontaneous activity, and energy expenditure accompanied muscle metabolic dysfunction atrophy. We identify Activin A as purported driver treat ActRIIB-Fc, decoy ligand for TGF-β/activin family members, together anamorelin (Ana), ghrelin receptor agonist, to reverse anorexia, respectively. Ana...
Exercise is a first-line treatment for type 2 diabetes and preserves β-cell function by hitherto unknown mechanisms. We postulated that proteins from contracting skeletal muscle may act as cellular signals to regulate pancreatic function. used electric pulse stimulation (EPS) induce contraction in C2C12 myotubes found of β-cells with EPS-conditioned medium enhanced glucose-stimulated insulin secretion (GSIS). Transcriptomics subsequent targeted validation revealed growth differentiation...
Individuals with idiopathic pulmonary arterial hypertension (PAH) display reduced oral glucose tolerance. This may involve defects in pancreatic function or insulin sensitivity but this hypothesis has not been tested; moreover, fasting nutrient metabolism remains poorly described PAH. Thus, we aimed to characterise and investigated the metabolic response hyperglycaemia 12 participants (six PAH, six controls) were administered a hyperglycaemic clamp, while 52 (21 31 underwent plasma...
Nonalcoholic fatty liver disease (NAFLD) is characterized by hepatic fat accumulation and impaired insulin sensitivity. Reduced ketogenesis may promote these pathologies, but data are inconclusive in humans the link between NAFLD reduced sensitivity remains obscure. We investigated individuals with obesity-related hypothesized that β-hydroxybutyrate (βOHB; predominant ketone species) would be related to Furthermore, we ketones impact skeletal muscle mitochondrial respiration vitro. Hepatic...
In efforts to evaluate potential biomarkers and drug targets for Neurofibromatosis Type I (NF1) we utilized affinity mass spectrometry global proteomics investigate how variation within loss of NF1 affect immortalized human Schwann cells. We used Strep tagged mNf1 cDNAs (both wild type (WT) variant) purity Protein-Protein interactors (PPIs) from the were able identify 98 PPIs show that some these bind differentially variant proteins. Next, evaluated proteomes. identified over 1900 proteins...
ABSTRACT Background Activation of mitochondrial fission and quality control occur early in the onset insulin resistance human skeletal muscle. We hypothesized that differences dynamics, structure bioenergetics humans would explain progression type 2 diabetes (T2D). Methods Fifty‐eight sedentary adults (37 ± 12 years) were enrolled into one three groups: (1) healthy weight (HW), (2) overweight obesity (Ow/Ob), or (3) T2D. Body composition, aerobic capacity, sensitivity assessed during a 3‐day...
Exercise seems to enhance the beneficial effect of bariatric (Roux-en-Y gastric bypass [RYGB]) surgery on insulin resistance. We hypothesized that skeletal muscle extracellular matrix (ECM) remodeling may underlie these benefits. Women were randomized either a combined aerobic and resistance exercise training program following RYGB (RYGB + ET) or standard care (RYGB). Insulin sensitivity was assessed by oral glucose tolerance test. Muscle biopsy specimens obtained at baseline 3 9 months...
Abstract Mitochondria are essential for survival and as such, impairments in organelle homeostasis significantly accelerate age‐related morbidity mortality. Here, we determined the contribution of bioenergetic efficiency to life span health Drosophila melanogaster utilizing mitochondrial uncoupler BAM15. Life was flies fed a normal diet (ND) or high fat (HFD) supplemented with vehicle Locomotor function by negative geotaxis assay middle‐aged BAM15 under ND HFD conditions. Redox capacity...
Metabolically healthy obesity (MHO) is often defined as the absence of metabolic syndrome in presence obesity. However, phenotypic features MHO are unclear. Insulin sensitivity was cross-sectionally compared with metabolically unhealthy (MUO) and a reference group young participants without obesity.Sedentary adults (n = 96) undergoing anthropometric, blood chemistries, maximal aerobic capacity, euglycemic-hyperinsulinemic clamp measurements were classified by BMI (<25 ≥30 kg/m2 ). MUO having...
Mutations in Valosin Containing Protein (VCP) are associated with several degenerative diseases, including multisystem proteinopathy (MSP-1) and amyotrophic lateral sclerosis. However, patients VCP mutations vary widely their pathology clinical penetrance, making it difficult to devise effective treatment strategies. A deeper understanding of how each mutation affects function could enhance the prediction outcomes design personalized options. The power a genetically tractable model organism...
Abstract Background Enhanced metabolic plasticity and diversification of energy production is a hallmark highly proliferative breast cancers. This contributes to poor pharmacotherapy efficacy, recurrence, metastases. We have previously identified mitochondrial-targeted furazano[3,4-b]pyrazine named BAM15 that selectively reduces bioenergetic coupling efficiency orally available. Here, we evaluated the antineoplastic properties uncoupling oxidative phosphorylation from ATP in cancer using...
Roux-en-Y gastric bypass (RYGB) and sleeve gastrectomy (SG) are effective procedures to treat manage type 2 diabetes (T2D). However, the underlying metabolic adaptations that mediate improvements in glucose homeostasis remain largely elusive. The purpose of this study was identify signatures associated with biochemical resolution T2D after medical therapy (MT) or bariatric surgery.
Dysregulation of gut microbiota and intestinal barrier function has emerged as potential mechanisms underlying digestive diseases, yet targeted therapies are lacking The purpose this investigation was to assess the efficacy UCC118, a characterized probiotic strain, on exercise-induced GI permeability in healthy humans. In randomized, double-blind, placebo-controlled crossover study, seven adults received 4 weeks daily UCC118 or placebo supplementation. hyperpermeability induced by strenuous...
The onset and progression of type 2 diabetes (T2D) results from failure to secrete sufficient amounts insulin overcome peripheral resistance. Here, we demonstrate that Roux-en-Y gastric bypass (RYGB) restores islet function morphology compared sham pair-fed controls in ZDF rats. improvements were largely attributable enhanced content secretory response glucose stimulation.