A5016189590- Natural product bioactivities and synthesis
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Plant biochemistry and biosynthesis
- Neonatal Respiratory Health Research
- Asthma and respiratory diseases
- Inhalation and Respiratory Drug Delivery
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Cellular Mechanics and Interactions
- Ginseng Biological Effects and Applications
- Marine Biology and Environmental Chemistry
- Cancer Cells and Metastasis
- Connexins and lens biology
- TGF-β signaling in diseases
- Heat shock proteins research
- Toxin Mechanisms and Immunotoxins
- Phytochemistry and Biological Activities
- Connective Tissue Growth Factor Research
- Microtubule and mitosis dynamics
- Polymer Surface Interaction Studies
- Cancer-related gene regulation
- Antioxidant Activity and Oxidative Stress
- Tissue Engineering and Regenerative Medicine
- Cancer, Hypoxia, and Metabolism
- Piperaceae Chemical and Biological Studies
- Surfactants and Colloidal Systems
Jagiellonian University
2015-2024
University of Agriculture in Krakow
2019
Institute of Biochemistry and Biophysics, Polish Academy of Sciences
2016
AGH University of Krakow
2014
Airway remodelling with subepithelial fibrosis, which abolishes the physiological functions of bronchial wall, is a major issue in asthma. Human fibroblasts (HBFs) derived from patients diagnosed asthma display vitro predestination towards TGF-β1-induced fibroblast-to-myofibroblast transition (FMT), key event fibrosis. As commonly used anti-asthmatic drugs do not reverse structural changes airways, and molecular mechanism enhanced asthma-related FMT poorly understood, we investigated balance...
Nanoparticles made of amphiphilic block copolymers are commonly used in the preparation nano-sized drug delivery systems. Poly(styrene)–block–poly(acrylic acid) (PS–PAA) have been proposed for purposes; however, loading capacity and cytotoxicity PS–PAA nanoparticles still not fully recognized. Herein, we investigated accumulation a model hydrophobic drug, curcumin, its spatial distribution inside nanoparticles. Experimental methods atomistic molecular dynamics simulations were to understand...
Investigating intestinal physiology in vitro remains challenging due to the lack of an effective primary enterocyte culture system. Recently developed protocols for growing organoids containing crypts and villus from adult mouse epithelium Matrigel present attractive alternative classical techniques. However, these approaches require use sophisticated expensive serum-free medium supplemented with epithelial growth factor (EGF), Wnt agonist (R-spondin 1), bone morphogenetic protein inhibitor...
Suppressive function of connexin(Cx)43 in carcinogenesis was recently contested by reports that showed a multifaceted Cx43 cancer progression. These studies did not attempt to model the dynamics intratumoral heterogeneity involved metastatic cascade. An unorthodox look at phenotypic prostate cells vitro enabled us identify links between functions and Snail-1-regulated functional speciation invasive cells. Incomplete Snail-1-dependent shifts accounted for formation phenotypically stable...
Section:ChooseTop of pageAbstract <<Materials and MethodsResultsDiscussionReferencesCITING ARTICLES
During asthma development, differentiation of epithelial cells and fibroblasts towards the contractile phenotype is associated with bronchial wall remodeling airway constriction. Pathological fibroblast-to-myofibroblast transition (FMT) can be triggered by local inflammation walls. Recently, we have demonstrated that human (HBFs) derived from asthmatic patients display some inherent features which facilitate their FMT in vitro. In spite intensive research efforts, these properties remain...
Subepithelial fibrosis is a component of the remodeling observed in bronchial wall patients diagnosed with asthma. In this process, human fibroblasts (HBFs) drive fibroblast-to-myofibroblast transition (FMT) response to transforming growth factor-β1 (TGF-β1), which activates canonical Smad-dependent signaling. However, pleiotropic properties TGF-β1 also promote activation non-canonical signaling pathways can affect FMT. study we investigated effect p38 mitogen-activated protein kinase (MAPK)...
Amphiphilic polycations with quaternary ammonium groups show a strong tendency to porate lipid membranes. The high capacity open pores in biomembranes are the most cytotoxic cells.
Objective: Extravasation of circulating cancer cells is an important step the metastatic cascade and a potential target for anti-cancer strategies based on vasoprotective drugs. Reports effects fenofibrate (FF) prompted us to analyze its influence endothelial barrier function during prostate cell diapedesis.
The activation of human bronchial fibroblasts by transforming growth factor-β₁ (TGF-β₁) leads to the formation highly contractile myofibroblasts in process fibroblast⁻myofibroblast transition (FMT). This is crucial for subepithelial fibrosis and wall remodeling asthma. However, this evades current therapeutic asthma treatment strategies. Since our previous studies showed attenuation TGF-β₁-induced FMT response lipid-lowering agents (e.g., statins), we were interested see whether a...
Oil-core nanocapsules (NCs, also known as nanoemulsions) are of great interest due to their application efficient carriers various lipophilic bioactives, such drugs. Here, we reported for the first time preparation and characterization NCs consisting chondroitin sulfate (CS)-based shells liquid oil cores. For this purpose, two amphiphilic CS derivatives (AmCSs) were obtained by grafting polysaccharide chain with octadecyl or oleyl groups. AmCS-based prepared an ultrasound-assisted...
Extracts from leaves and stems of Chenopodium hybridum were characterised for the presence quantity flavonoids phenolic acids by LC-ESI-MS/MS. Five eight detected first time in aerial parts this plant species, most abundant compounds being rutin (2.80 μg/g DW), 3-kaempferol rutinoside (2.91 4-OH-benzoic (1.86 DW) syringic (2.31 DW). tested anti-inflammatory/antiarthritic, antihyaluronidase cytotoxic activities against human prostate cancer (Du145, PC3) melanoma cell lines (A375, HTB140...
Abstract Background The asthma-related airway wall remodeling is associated i.a. with a damage of bronchial epithelium and subepithelial fibrosis. Functional interactions between human epithelial cells fibroblasts are known as the epithelial-mesenchymal trophic unit (EMTU) necessary for proper functioning lung tissue. However, high concentration transforming growth factor-β 1 (TGF-β ) in asthmatic bronchi drives structural disintegrity epithelial-to-mesenchymal transition (EMT) cells,...