A5101665859- Knee injuries and reconstruction techniques
- Total Knee Arthroplasty Outcomes
- RNA and protein synthesis mechanisms
- Cancer Treatment and Pharmacology
- Biological Activity of Diterpenoids and Biflavonoids
- RNA modifications and cancer
- Synthetic Organic Chemistry Methods
- Orthopaedic implants and arthroplasty
- Lower Extremity Biomechanics and Pathologies
- Microbial Natural Products and Biosynthesis
- Bacterial Genetics and Biotechnology
- Bioactive Compounds and Antitumor Agents
- Neurobiology and Insect Physiology Research
- Sports injuries and prevention
- Synthesis and biological activity
- Enzyme Catalysis and Immobilization
- Chromosomal and Genetic Variations
- Marine Sponges and Natural Products
- Cardiovascular Effects of Exercise
- RNA Interference and Gene Delivery
- Chemical synthesis and alkaloids
- Foot and Ankle Surgery
- Carbohydrate Chemistry and Synthesis
- Yeasts and Rust Fungi Studies
- Glycosylation and Glycoproteins Research
The University of Tokyo
1999-2024
Ochanomizu University
2024
University of Tokyo Hospital
2024
Kitasato University
2021
Niigata University
2011-2021
Kindai University
2021
Tokyo University of Pharmacy and Life Sciences
2019
Kansas State University
2018
Kyoto Institute of Technology
2018
Massachusetts General Hospital
2017
Abstract Argonaute proteins are key players in the gene silencing mechanisms mediated by small nucleic acids all domains of life from bacteria to eukaryotes. However, little is known about protein that recognizes guide RNA/target DNA. Here, we determine 2 Å crystal structure Rhodobacter sphaeroides ( Rs Ago) a complex with 18-nucleotide RNA and its complementary target The heteroduplex maintains Watson–Crick base-pairing even 3′-region between N-terminal PIWI domains, suggesting recognition...
Background: Previous studies have attempted to determine prognostic factors for predicting the occurrence of noncontact anterior cruciate ligament (ACL) injuries. However, on risk ACL injuries in Asian female soccer players are limited. Purpose: To identify intrinsic among young players. Study Design: Cohort study; Level evidence, 2. Methods: This study evaluated 145 Japanese potential injury during preseason medical assessment. In total, 25 variables were examined—including anthropometric...
In all organisms, the large ribosomal subunit contains multiple copies of a flexible protein, so-called 'stalk'. The C-terminal domain (CTD) stalk interacts directly with translational GTPase factors, and this interaction is required for factor-dependent activity on ribosome. Here we have determined structure complex CTD archaeal protein aP1 GDP-bound elongation factor aEF1α at 2.3 Å resolution. showed that formed long extended α-helix, which bound to cleft between domains 1 3 aEF1α, bridged...
Ribosomal stalk proteins recruit translation elongation GTPases to the factor-binding center of ribosome. Initiation factor 5B (eIF5B in eukaryotes and aIF5B archaea) is a universally conserved GTPase that promotes joining large small ribosomal subunits during initiation. Here we show binds C-terminal tail protein. In cocrystal structure, interaction occurs between hydrophobic amino acids pocket on surface GTP-binding domain (domain I) aIF5B. A substitution mutation altering yeast eIF5B...
Peptidyl-tRNA hydrolase (Pth) cleaves the ester bond between peptide and tRNA of peptidyl-tRNA molecules, which are produced by aborted translation, to recycle for further rounds protein synthesis. Pth is ubiquitous in nature, its enzymatic activity essential bacterial viability. We have determined crystal structure Escherichia coli complex with CCA-acceptor-TΨC domain, enzyme-binding region moiety substrate, at 2.4 Å resolution. In combination site-directed mutagenesis studies, identified...
Abstract Purpose The tibial anatomical anteroposterior (AP) axis “Akagi's line” was originally defined on computed tomography (CT) in total knee arthroplasty (TKA); however, its intraoperative reproducibility remains unknown. This study aimed to evaluate the of Akagi's line and effect postoperative clinical outcomes. Methods prospective included 171 TKAs. rotational angle relative original (RAA) CT measured. RAA calculated based component angles measured using navigation system CT. effects...
This study aimed to evaluate the effects of differences in initial graft tension (IGT) on patellofemoral alignment axial plane after anatomical anterior cruciate ligament reconstruction (ACLR).
The piRNA amplification pathway in Bombyx is operated by Ago3 and Siwi their piRISC form. DEAD-box protein, Vasa, facilitates Ago3-piRISC production liberating cleaved RNAs from Siwi-piRISC an ATP hydrolysis-dependent manner. However, the Vasa-like factor facilitating along this remains unknown. Here, we identify polypeptide 43 (DDX43) as protein functioning production. DDX43 belongs to helicase superfamily II with it contains a similar core. also K-homology (KH) domain, prevalent...
Two types of elongation factors alternate in their binding to the factor-binding center ribosome. Both events are accompanied by GTP hydrolysis and drive translation cycle. The multicopy ribosomal protein family, termed stalk, contributes actively process. Recent evidence indicates that mobile C-terminal tail archaeal stalk aP1 directly interacts with both aEF1A aEF2. To investigate functional significance these interactions recruitment ribosome, we substituted complex aL10•aP1 for bL10•bL12...
Background: Previous studies have attempted to determine if certain risk factors can predict the occurrence of a lateral ankle sprain (LAS) in female soccer players. Unfortunately, there is limited evidence with regard associated an LAS Purpose: To identify intrinsic for among young Study Design: Cohort study; Level evidence, 2. Methods: Participants were 161 players Japan who evaluated during preseason medical assessment. The assessment included anthropometric, joint laxity, range motion,...
State-dependent modulation of sensory systems has been studied in many organisms and is possibly mediated through neuromodulators such as monoamine neurotransmitters. Among these, dopamine involved aspects animal behaviour, including movement control, attention, motivation cognition. However, the precise neural mechanism underlying dopaminergic behaviour induced by stimuli remains poorly understood. Here, we used Drosophila melanogaster to show that can modulate optomotor response moving...
Previous studies have aimed to determine the use of certain risk factors in predicting occurrence noncontact anterior cruciate ligament (ACL) injuries. Unfortunately, evidence regarding ACL injuries male American football players is limited. This prospective cohort study identify intrinsic for injury among players. evaluated 152 Japan potential during a preseason medical assessment, including anthropometric, joint laxity, and flexibility, muscle strength, balance measurements. A total 25...
Peptidyl-tRNA hydrolase (Pth) cleaves the ester bond between peptide and tRNA of peptidyl-tRNA molecules, which are product aborted translation. In present work, Pth from Escherichia coli was crystallized with acceptor-TΨC domain using 1,4-butanediol as a precipitant. The crystals belonged to hexagonal space group P 6 1 , unit-cell parameters = b 55.1, c 413.1 Å, diffracted X-rays beyond 2.4 Å resolution. asymmetric unit is expected contain two complexes ( V M 2.8 3 Da −1 ), solvent content...
Six new taxane diterpenoids and three known 2(3-->20)abeotaxoids were isolated from the seeds of Taxus chinensis var. mairei yunnanensis. Their structures established as 2alpha, 20-dihydroxy-9alpha-acetoxytaxa-4(20),11-dien-13-one (1), 5alpha-cinnamoyloxy-7beta,10beta, 13alpha-triacetoxy-2(3-->20)abeotaxa-2alpha-ol-4(20),11-dien-9-one (2), 5alpha-cinnamoyloxy-10beta, 13alpha-diacetoxy-2(3-->20)abeotaxa-2alpha,7beta-diol-4(20), 11-dien-9-one (3), 5alpha-cinnamoyloxy-2alpha,7beta,10beta,...
The palm oil mill effluent (POME) is generally treated using biological anaerobic treatment and then utilized for POME land application. This research aimed to study the effect of application on soil microbial community structure in plantation their impact productivity. results showed that ubiquinone (UQ) menaquinone (MK) contents soils without were 0.045 0.204 μmol/kg-dry soils, respectively. These much lower than UQ MK with which 0.074 0.301 μmol/ kg-dry diversity quinone (DQ) bioenergetic...
Initiation factor 5B (IF5B) is a universally conserved translational GTPase that catalyzes ribosomal subunit joining. In eukaryotes, IF5B directly interacts via groove in its domain IV with initiation 1A (IF1A), another factor, to accomplish efficient Here, we have determined the first structure of crenarchaeal IF5B, which revealed archaea-specific region (helix α15) binds and occludes IV. Therefore, archaeal cannot access IF1A same manner as eukaryotic IF5B. This fact suggests different...