A5101962415- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Algal biology and biofuel production
- Reconstructive Surgery and Microvascular Techniques
- Ubiquitin and proteasome pathways
- Breast Implant and Reconstruction
- Reconstructive Facial Surgery Techniques
- Aquatic Ecosystems and Phytoplankton Dynamics
- Xenotransplantation and immune response
- RNA modifications and cancer
- RNA Interference and Gene Delivery
- Cancer-related gene regulation
- Neonatal Respiratory Health Research
- Pluripotent Stem Cells Research
- T-cell and B-cell Immunology
- Innovative Microfluidic and Catalytic Techniques Innovation
- Chemical Synthesis and Analysis
- Advanced Thermodynamics and Statistical Mechanics
- RNA Research and Splicing
- Gene Regulatory Network Analysis
- Immunotherapy and Immune Responses
- Fish Ecology and Management Studies
- Asymmetric Hydrogenation and Catalysis
- Histone Deacetylase Inhibitors Research
- Carbohydrate Chemistry and Synthesis
Pontificia Universidad Católica de Valparaíso
2025
Pfizer (United States)
2005-2024
Justus-Liebig-Universität Gießen
2024
Czech Academy of Sciences, Biology Centre
2021-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2023
Centre National de la Recherche Scientifique
2020-2023
Sorbonne Université
2017-2023
Université Côte d'Azur
2017-2023
Institute of Hydrobiology, Biology Centre, Academy of Sciences of the Czech Republic
2022
Czech Academy of Sciences
2022
Ancestral sequence reconstruction (ASR) has been used to produce enzymes that exhibit enhanced stability, activity, and promiscuity compared wild-type (WT) enzymes. In this work, we compiled a panel of 57 closely related ene reductases 56 counterpart ancestral statistically their biocatalytic properties. Enzyme selectivity, thermostability, expression level distributions were compare performance. ASR WT exhibited comparable activity overexpression levels, but displayed average...
A new manufacturing process for (S)-3-(aminomethyl)-5-methylhexanoic acid (Pregabalin), the active ingredient in Lyrica, has been developed. Using Lipolase, a commercially available lipase, rac-2-carboxyethyl-3-cyano-5-methylhexanoic ethyl ester (1) can be resolved to form 2-carboxyethyl-3-cyano-5-methylhexanoic (2). heat-promoted decarboxylation of 2 efficiently generates (S)-3-cyano-5-methylhexanoic (3), known precursor Pregabalin. This route dramatically improved efficiency compared...
ADVERTISEMENT RETURN TO ISSUEViewpointNEXTEmerging Technologies for Biocatalysis in the Pharmaceutical IndustryRussell D. Lewis*Russell LewisPfizer Worldwide Research and Development, 445 Eastern Point Road, Groton, Connecticut 06340, United States*[email protected]More by Russell Lewishttps://orcid.org/0000-0002-5776-7347, Scott P. FranceScott FrancePfizer StatesMore Francehttps://orcid.org/0000-0003-1679-6165, Carlos A. MartinezCarlos MartinezPfizer MartinezCite this: ACS Catal. 2023, 13,...
Abstract Purpose: T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive disease, affecting children and adults. Chemotherapy treatments show high response rates but have debilitating effects carry risk of relapse. Previous work implicated NOTCH1 other oncogenes. However, direct inhibition these pathways affects healthy tissues cancer alike. Our goal in this has been to identify enzymes active T-ALL whose activity could be targeted for therapeutic purposes. Experimental Design: To...
Abstract Purpose: Multisite stereotactic body radiotherapy followed by pembrolizumab (SBRT+P) has demonstrated safety in advanced solid tumors (ASTs). However, no studies have examined the relationships between irradiated tumor response, SBRT-induced gene expression, and overall survival (OS). Patients Methods: with AST received SBRT (30–50 Gy 3–5 fractions) to two four metastases (200 mg i.v. every 3 weeks). was prescribed a maximum volume of 65 mL. Small complete coverage (complete-Rx),...
Cyclohexanone monooxygenase (E.C. 1.14.13.22) from Acinetobacter sp. NCIB 9871 has been expressed in baker's yeast (Saccharomyces cerevisiae) to create a general reagent for asymmetric Baeyer−Villiger oxidations. This "designer yeast" approach combines the advantages of using purified enzymes (single catalytic species, no overmetabolism, etc.) with benefits whole-cell reactions (experimentally simple, cofactor regeneration necessary, etc.). The was used systematically examine series 2-, 3-,...
Background. Removal of the rib and adjacent cartilage is a common step for exposure recipient chest vessels in free-flap breast reconstructions. However, this adds both short- long-term morbidity to procedure. We describe our experience avoiding removal microvascular reconstruction. Patients Methods. retrospectively reviewed vessel preparation reconstructions performed by single surgeon (SGB). Results. A total 556 consecutive patients, totaling 1106 flaps over 5 years, were assessed....
1. Tolerance of male skin isografts has been regularly produced in female mice the C57B1 strain sublines 1, 4, and 6 during adult life by repeated injection completely disrupted spleen cells derived from donors. The tolerant state is long-lasting since such grafts have remained place more than 9 months. 2. Prolonged survival homotransplants DBA/2 injections Balb/C When cell material are continued over a sufficiently long period, permanent acceptance homografts may be obtained between these...
Sickle cell disease (SCD) is a genetic disorder caused by single point mutation (β6 Glu → Val) on the β-chain of adult hemoglobin (HbA) that results in sickled (HbS). In deoxygenated state, polymerization HbS leads to sickling red blood cells (RBC). Several downstream consequences and RBC include vaso-occlusion, hemolytic anemia, stroke. We report design noncovalent modulator HbS, clinical candidate PF-07059013 (23). The seminal hit molecule was discovered virtual screening confirmed through...
PURPOSE To determine the maximal-tolerated dose (MTD) of paclitaxel in combination with high-dose cyclophosphamide (CPA) and cisplatin (cDDP) followed by autologous hematopoietic progenitor-cell support (AHPCS). PATIENTS AND METHODS Forty-nine patients poor-prognosis breast cancer, non-Hodgkin's lymphoma (NHL), or ovarian cancer were treated escalating doses infused over 24 hours, CPA (5,625 mg/m2 intravenously 1 hour three divided doses) cDDP (165 as a continuous infusion 72 hours) AHPCS....
1. The capacity of spleen cells from mice thymectomized during neonatal life to elicit graft vs host reactions was studied using Simonsen's assay histocompatibility. 2. reaction A strain sham operated or in the period compared that isologous F1 (A × C57B1)F1 recipients and Z similarly treated groups were (Z C57B1) DBA/2)F1 hybrids. 3. results show early complete thymectomy both renders these animals incapable producing Fl 4. These data are interpreted as evidence themselves lympho-reticular...
Synthesis of (S)-5-fluoro-3-methylisobenzofuran-1(3H)-one (6), a key intermediate to lorlatinib, is described. A few synthetic methodologies, that is, boron reduction, enzymatic asymmetric hydrogenation, and transfer were evaluated for the chiral reduction substituted acetophenone (8). manufacturing process, on basis was developed. This process successfully scaled up prepare 400 kg 6.
Breast reconstruction with autologous tissue is considered the current state-of-the-art choice following mastectomies, and deep inferior epigastric perforator (DIEP) flap often among favored techniques. Commonly referred to patients as a combination between tummy tuck breast augmentation, it significantly differs by required expertise long hospital stays. We present series attesting feasibility effectiveness of performing this type in an outpatient setting our recovery protocol.Patients...
The extensive nature of perforator-based breast reconstructions, combined with the need for postoperative flap monitoring, often leads to long hospitalizations. We present an early report demonstrating feasibility and advantages a modified operative technique recovery protocol, allowing us perform outpatient reconstructions DIEP flap. This follow-up comprises experience gained, which is expanded other flaps not limited reconstructions.We have implemented general protocol in patients...
It is difficult to understand the composition and diversity of biological communities in complex heterogeneous environments using traditional sampling methods. Recently, developments environmental DNA metabarcoding have emerged as a powerful, non-invasive method for comprehensive community characterization biodiversity monitoring different types aquatic ecosystems. In this study, water eDNA targeting fish (wf-eDNA) four methods (electrofishing, gill netting, seining, trawling) were compared...