A5060351153- Genomics and Chromatin Dynamics
- DNA Repair Mechanisms
- Ubiquitin and proteasome pathways
- Metabolomics and Mass Spectrometry Studies
- RNA and protein synthesis mechanisms
- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Endoplasmic Reticulum Stress and Disease
- Cellular transport and secretion
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Fungal and yeast genetics research
- Historical and Environmental Studies
- Nuclear Structure and Function
- RNA Research and Splicing
- Microtubule and mitosis dynamics
- Parasitic Infections and Diagnostics
- Glycosylation and Glycoproteins Research
- Cardiomyopathy and Myosin Studies
- Diverse academic and cultural studies
- Protein Degradation and Inhibitors
- Italian Social Issues and Migration
- Pluripotent Stem Cells Research
- Bacterial Genetics and Biotechnology
- Lipid Membrane Structure and Behavior
IFOM
2012-2020
European Institute of Oncology
2015
University of Milan
2003-2005
National Research Council
2003
Istituto Nazionale di Fisica Nucleare, Sezione di Milano
2003
AbstractUbiquitination of histones plays a critical role in the regulation several processes within nucleus, including maintenance genome stability and transcriptional regulation. The only known ubiquitination site on is represented by conserved Lys residue located at C terminus protein. Here, we describe novel ubiquitin mark N-terminal tail histone H2As consisting two residues positions 13 15 (K13/K15). This “bidentate” target DNA damage response (DDR) ligases RNF8 RNF168. Histone mutants...
Unrepaired DNA damage during embryonic development can be potentially inherited by a large population of cells. However, the quality control mechanisms that minimize contribution damaged cells to developing embryos remain poorly understood. Here, we uncovered an ATR- and CHK1-mediated transcriptional response replication stress (RS) in mouse stem (ESCs) induces genes expressed totipotent two-cell (2C) stage 2C-like This is mediated Dux, multicopy retrogene defining cleavage-specific program...
C-tail-anchored proteins are defined by an N-terminal cytosolic domain followed a transmembrane anchor close to the C terminus. Their extreme C-terminal polar residues translocated across membranes poorly understood post-translational mechanism(s). Here we have used yeast system study translocation of terminus tagged form mammalian cytochrome<i>b</i> <sub>5</sub>, carrying <i>N</i>-glycosylation site in its (<i>b</i> <sub>5</sub>-Nglyc). Utilization this was adopted as rigorous criterion for...
Proximity-dependent trans-biotinylation by the Escherichia coli biotin ligase BirA mutant R118G (BirA*) allows stringent streptavidin affinity purification of proximal proteins. This so-called BioID method provides an alternative to widely used co-immunoprecipitation (co-IP) identify protein-protein interactions. Here, we BioID, on its own and combined with co-IP, proteins involved in nonsense-mediated mRNA decay (NMD), a post-transcriptional turnover pathway that targets mRNAs fail...
Abstract Ubiquitination is a highly dynamic and versatile posttranslational modification that regulates protein function, stability, interactions. To investigate the roles of ubiquitination in primitive eukaryotic lineage, we utilized early‐branching eukaryote G iardia intestinalis . Using combination biochemical, immunofluorescence‐based, proteomics approaches, assessed status during process differentiation We observed different types ubiquitin modifications present specific cellular...
Cell survival to replication stress depends on the activation of Mec1ATR-Rad53 checkpoint response that protects integrity stalled forks and controls origin firing program. Here we found Mad2, a member spindle assembly (SAC), contributes efficient cell in stress. We show Rad53 Mad2 promote S-phase cyclin expression through different mechanisms: while influences Clb5,6 degradation, promotes their protein synthesis. co-sediments with polysomes modulates association translation inhibitor...
Histone deacetylases (HDACs) are modification enzymes that regulate a plethora of biological processes. HDAC1, crucial epigenetic modifier, is deregulated in cancer and subjected to variety post-translational modifications. Here, we describe the generation new monoclonal antibody specifically recognizes novel highly dynamic prophase phosphorylation serine 406-HDAC1, providing powerful tool for detecting early mitotic cells.
Summary Unrepaired DNA damage during embryonic development can be potentially inherited by a large population of cells. However, the quality control mechanisms that minimize contribution damaged cells to developing embryos remain poorly understood. Here, we uncovered an ATR- and CHK1-mediated transcriptional response replication stress (RS) in ESCs induces genes expressed totipotent two-cell (2C) stage 2C-like This is mediated Dux, multicopy retrogene defining cleavage-specific program...