A5058469735- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Genomics and Chromatin Dynamics
- Cancer-related molecular mechanisms research
- Mesenchymal stem cell research
- Adenosine and Purinergic Signaling
- Cancer Immunotherapy and Biomarkers
- Alzheimer's disease research and treatments
- RNA modifications and cancer
- Glioma Diagnosis and Treatment
- Tissue Engineering and Regenerative Medicine
- Cancer Genomics and Diagnostics
- Pluripotent Stem Cells Research
- Medicinal Plants and Bioactive Compounds
- Chromatin Remodeling and Cancer
- Axon Guidance and Neuronal Signaling
- Renal and related cancers
- Peroxisome Proliferator-Activated Receptors
- Microtubule and mitosis dynamics
- Sleep and Wakefulness Research
- Immune Cell Function and Interaction
- Adipose Tissue and Metabolism
- Regulation of Appetite and Obesity
- Cytokine Signaling Pathways and Interactions
University of Calgary
2020-2024
Alberta Children's Hospital
2020-2024
University of British Columbia
2014-2021
Yonsei University
2010-2014
Several species of intestinal bacteria have been associated with enhanced efficacy checkpoint blockade immunotherapy, but the underlying mechanisms by which microbiome enhances antitumor immunity are unclear. In this study, we isolated three bacterial species-Bifidobacterium pseudolongum, Lactobacillus johnsonii, and Olsenella species-that significantly immune inhibitors in four mouse models cancer. We found that B. pseudolongum modulated immunotherapy response through production metabolite...
Posterior fossa group A (PFA) ependymoma is a lethal brain cancer diagnosed in infants and young children. The lack of driver events the PFA linear genome led us to search its 3D for characteristic features. Here, we reconstructed genomes from diverse childhood tumor types uncovered global topology that highly reminiscent stem progenitor cells variety human tissues. remarkable feature exclusively present are type B ultra long-range interactions PFAs (TULIPs), regions separated by great...
Objectives: This study has aimed to repopulate 'primitive' cells from late-passage mesenchymal stem (MSCs) of poor multipotentiality and low cell proliferation rate, by simply altering plating density.Materials methods: Effects density culture compared t high on bone marrow (BM)-derived MSCs pluripotency markers were investigated.Cell proliferation, gene expression, RNA interference differentiation potential assayed.Results conclusions: We repopulated replating at (17 ⁄ cm 2 ) regardless...
The chondrogenic differentiation process of human mesenchymal stem cells (hMSCs) passes through multiple stages, which are carried out by various factors and their interactions. Recently, microRNAs that regulate have been reported. However, microRNA regulates SRY-related high mobility group-box gene 9 (Sox9), a key factor, has not identified in hMSC. In this study, we microRNA-495 (miR-495) is an important regulator hMSC differentiation. our microarray, miR-495 was downregulated during...
microRNAs are small molecules, about 17-23 nucleotides in length, that act as translational regulators of their target gene. By binding to a target, known either inhibit translation or induce degradation the target. Despite great interest microRNAs, however, exact targets each individual microRNA different processes remain largely unknown. In this study, we determined lymphoid enhancer-binding factor-1 (LEF-1) was expressed during chondrogenesis human bone marrow-derived mesenchymal stem...
Cyclooxygenase-2 (COX-2) inhibitors suppress bone repair and formation by suppressing angiogenesis as well potentially interfering with osteoblast osteoclast functions. In spite of these reports, there is a controversy over the exact effects COX-2 on processes itself. This work was designed to investigate effect inhibitor osteogenesis human marrow-derived mesenchymal stem cells (MSC). MSCs in were treated (celecoxib naproxen) absence or presence interleukin-1β (IL-1β), which used induce...
Adipogenesis is largely dependent on the signal transducers and activators of transcription (STAT) pathway. However, molecular mechanism STAT pathway in adipogenesis human bone marrow-derived stromal cells (hBMSCs) remains not well understood. The purpose this research was to characterize transcriptional regulation involved expression STAT5A STAT5B during hBMSCs 3T3-L1 cells. increases with onset PPAR response elements regulatory element exists at a promoter region ranging from -346 -101,...
Self-renewal is a crucial property of glioblastoma cells that enabled by the choreographed functions chromatin regulators and transcription factors. Identifying targetable epigenetic mechanisms self-renewal could therefore represent an important step toward developing effective treatments for this universally lethal cancer. Here we uncover axis mediated histone variant macroH2A2. With omics functional assays deploying patient-derived in vitro vivo models, show macroH2A2 shapes accessibility...
Somatostatin is involved in the regulation of multiple signaling pathways and affords neuroprotection response to neurotoxins. In present study, we investigated role Somatostatin-14 (SST) cell viability phosphorylation Collapsin Response Mediator Protein 2 (CRMP2) (Ser522) via blockade Ca2+ accumulation, along with inhibition cyclin-dependent kinase 5 (CDK5) Calpain activation differentiated SH-SY5Y cells. Cell Viability Caspase 3/7 assays suggest that presence SST ameliorates mitochondrial...
Summary Cancer is a leading cause of death globally. Checkpoint blockade therapies offer promising treatment for many cancers, but have been ineffective colorectal cancers. Previous studies shown dependency immunotherapies on the microbiota. Consequently, we hypothesized that specific gut bacteria promote immunotherapy cancer. We identify three commensal and microbial metabolite, inosine, enhance efficacy immune checkpoint therapy in show inosine interacts with adenosine A 2A receptor T...
Abstract Posterior Fossa Group A (PFA) ependymomas are pediatric brain tumors with extremely poor survival outcomes. As protein-coding mutations in PFA exceedingly rare, the underlying etiology of these remains elusive. Elevated CpG island methylation and depletion H3K27me3 have been described PFA, leading to hypothesis that may be driven by a dysregulated epigenetic state. In this study, we sought determine how three-dimensional (3D) genome features (such as DNA loops, domains,...
Abstract Glioblastoma is a highly malignant tumour driven by subset of self-renewing cells termed glioblastoma stem cells. This self-renewal phenotype largely epigenetically driven, however, the exact epigenetic mechanisms underpinning it are poorly understood. We found that histone variant macroH2A2 repressed in tumours, and this repression associated with poorer survival. interrogate function using vitro vivo patient-derived models. show antagonizes expression NPC OPC-like markers, rewires...
SUMMARY Self-renewal is a crucial property of glioblastoma cells and enabled by the choreographed function chromatin regulators transcription factors. Identifying targetable epigenetic mechanisms self-renewal could represent an important step toward developing new effective treatments for this universally lethal cancer. Here we uncover axis mediated histone variant macroH2A2. Using patient-derived in vitro vivo models, show that macroH2A2 has direct role shaping accessibility at enhancer...