Triple-negative breast cancer (TNBC) has the worst prognosis of all subtypes (BC), with limited options for conventional therapy and no targeted therapies. MicroRNAs (miRNAs) are small noncoding RNAs that negatively regulate gene expression. In this study, we aimed to determine whether two members miR-200 family, miR-200b-3p miR-429-5p, involved in BC cell proliferation motility elucidate their target genes pathways. We performed a meta-analysis reveals down-regulated expression miR-429-5p...

Dysregulation of microRNAs (miRNAs) plays a critical role in cancer progression. They can act as either oncogenes or tumor suppressor genes human cancer. The purpose this study was to investigate the crucial miR-135b breast and validate whether could regulate proliferation cells by effecting specific targets Hippo pathway. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) carried out quantify expression levels both tissues cell lines. To characterize function miR-135b,...

Understanding host responses to viral gene therapy vectors is necessary for the development of safe and efficacious in vivo transfer agents. We describe use high-density spotted complementary DNA microarrays monitoring transcriptional mouse liver upon administration either a "first-generation"adenoviral (Ad) vector, helper-dependent "gutless" adenoviral (HD) or an adeno-associated (AAV) vector containing human factor IX (hFIX) expression cassettes. Since HD AAV do not contain any genes, they...

Naturally occurring CD4+CD25+FoxP3+ regulatory T cells (Treg) suppress helper (Th) cell-mediated immune responses. The cytokines IL-2 and IL-6 are known to influence Treg function. However, their relative effects on Th versus not well understood. Stimulation with IL-2, a lesser extent, IL-6, enhanced proliferation, FoxP3 CTLA4 maintenance, suppressive In contrast, when or were added Treg/Th cell cocultures, suppression was inhibited. molecule SOCS3 negatively regulates responses IL-6....

The purpose of this study was to examine the expression levels microRNA-7 (miR-7) in human thyroid papillary cancer and its potential role disease pathogenesis. different miRNAs were detected by miRNA-microarray analysis ten specimens adjacent normal tissues. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) conducted determine level miR-7 both tissues cell lines. To characterize function miR-7, MTT assay, colony formation migration invasion cycle assay apoptosis used....

The aim of the present study was to define function microRNA‑424‑5p (miR‑424) in breast cancer cells. investigated level and potential miR‑424 by reverse transcription‑quantitative polymerase chain reaction assays. expression decreased majority human specimens cell lines used study. MTT assay, plate colony formation assay flow cytometry analyses were characterize two types lines. Upregulation inhibited cellular proliferation regulated cycle arresting cells G2/M phase. dual‑luciferase...

ABSTRACT Previously we showed that recombinant adenoviral helper-dependent (HD) vectors result in long-term transgene expression levels vivo which slowly declined by 95% over a period of 1 year. In this study, further establish was not predominantly immune mediated. To determine if cell turnover responsible for the loss expression, induced rapid hepatocyte cycling mouse liver, performing surgical two-thirds partial hepatectomy. We observed 55 and 65% reduction 50 71% vector genomes HD...

Abstract In vivo studies have suggested that gradients of CXCL12 (aka stromal cell‐derived factor 1α) may be critical for neural stem cell (NSC) migration during brain development and tissue regeneration. However, traditional in vitro chemotaxis tools are limited by unstable concentration the inability to decouple directionality speed. These limitations restricted reproducible quantitative analysis neuronal migration, which is required mechanism‐based studies. Using a microfluidic gradient...

MicroRNAs (miRNAs/miRs) are 19-25 nucleotide-long, non-coding RNAs that regulate the expression of target genes at post-transcriptional level. In present study, role miR-340 in breast cancer (BC) was investigated. The overexpression significantly inhibited proliferation, migration and invasion human MDA-MB-231 cells vitro. Rho-associated, coiled-coil containing protein kinase 1 (ROCK1) gene identified as a miR-340; its downregulated by binding to 3'-untranslated region. short interfering...

This study aimed to present evidence that miR-7 inhibited the metastasis of breast cancer stem cells (BCSCs) and elucidated mechanisms have remained unknown. The samples collected from agomir-treated, BCSC-driven tumors were subjected a protein array analyze expression profiles. A dual-luciferase reporter chromatin immunoprecipitation-PCR used validate evaluate molecular expressions interest in tissues cell lines. overexpression affecting BCSCs was further evaluated mice. endothelial...

Breast cancer patients with high expression of aldehyde dehydrogenases (ALDHs) cell population have higher tolerability to chemotherapy since the cells posses a characteristic breast stem (BCSCs) that are resistant conventional chemotherapy. In this study, we found ALDH-positive were in CD44+ CD24- and ESA+ BCSCs than both BT549 MDA-MB-231 lines but microRNA-7 (miR-7) level was lower cells. Moreover, miR-7 overexpression decreased ALDH1A3 activity by directly binding 3'-untranslated region...

Fusarium head blight (FHB), a destructive fungal disease that can cause damage to various crops and reduce yield quality, is primarily caused by several species of the soil-borne genus Fusarium, which produce mycotoxins contaminate grains may severe chronic diseases in humans livestock. In recent years, Bacillus spp. have been reported be good producers antifungal antibiotics against FHB. This study aimed explore potential role newly identified strain, designated as CU-XJ-9, was isolated...

Temporomandibular joint osteoarthritis (TMJOA) is a common inflammatory disease that can cause pain, cartilage degradation, and subchondral bone loss. However, the key regulatory factors new targets for treatment of TMJOA have yet to be determined. Long noncoding RNAs (lncRNAs) shown remarkable potential in regulating tissue homeostasis development. The long intergenic RNA-erythroid prosurvival (lincRNA-EPS) reported an effective inhibitor inflammation, but its role unexplored. Here, we...

A microfluidic-based screen to identify<italic>Salmonella</italic>genes that impede dendritic cell chemotaxis, a critical step of the human immune response.

The novel pyrazoline derivative, BHX, has recently been shown to exhibit potent anti-tumour activity by blocking the Wnt/β-catenin signalling pathway. However, its effect on breast cancer growth and invasion are unknown. Our results show that BHX suppresses MDA-MB-231 cell viability colony formation in a dose-dependent manner, induces apoptosis G0/G1 phase arrest. BHX-treated cells showed morphological characteristics of undergoing apoptosis. Furthermore, inhibited migration invasion, which...

ABSTRACT A digital coding metasurface is a platform connecting the space and electromagnetic wave space, has therefore gained much attention due to its intriguing value in reshaping wireless channels realizing new communication architectures. Correspondingly, there an urgent need for information theory that reveals upper limit of capacity supports accurate design metasurface-based systems. To this end, we propose macroscopic model statistical metasurface. The uniformly accommodates both...

Abstract Sepsis is defined as infection with organ dysfunction due to a dysregulated immune response. The lung one of the most vulnerable organs at onset sepsis. Interleukin (IL)‐33 can be released by injured epithelial and endothelial cells in regulate activation infiltration. Therefore, we postulated that IL ‐33 would contribute response during Using cecal ligation puncture ( CLP ) sepsis model, show here contributes significantly both sepsis‐induced inflammation systemic inflammatory...

Psoriasis is a chronic inflammatory skin disorder that impairs the quality of life affected patients. Emerging studies indicate certain long non-coding RNAs (lncRNAs) have important roles in psoriasis. However, exact functions lncRNAs and their regulatory mechanisms as competitive endogenous (ceRNAs) psoriasis remained to be fully elucidated. In present study, differentially expressed lncRNAs, microRNAs (miRNAs) mRNAs were identified by analyzing public datasets, psoriasis-associated...