A5078156040- Autoimmune Bullous Skin Diseases
- Reproductive System and Pregnancy
- Urticaria and Related Conditions
- Platelet Disorders and Treatments
- T-cell and B-cell Immunology
- Pregnancy and Medication Impact
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Cell Adhesion Molecules Research
- Immune Cell Function and Interaction
- Hedgehog Signaling Pathway Studies
- Genetic and Kidney Cyst Diseases
- Mycobacterium research and diagnosis
- Prenatal Screening and Diagnostics
- Immunotherapy and Immune Responses
- Dermatology and Skin Diseases
- Nail Diseases and Treatments
- Cytokine Signaling Pathways and Interactions
- Cancer-related molecular mechanisms research
- Preterm Birth and Chorioamnionitis
- Air Quality and Health Impacts
- Fetal and Pediatric Neurological Disorders
- Monoclonal and Polyclonal Antibodies Research
- Effects and risks of endocrine disrupting chemicals
- Pregnancy and preeclampsia studies
- Endometriosis Research and Treatment
Shanghai Tenth People's Hospital
2018-2024
Tongji University
2018-2024
University of Pennsylvania
2024
Shanghai Jiao Tong University
2014-2021
Qingdao Women and Children's Hospital
2020
Qingdao University
2020
Ruijin Hospital
2014-2019
Decidual CD4+ T (dCD4 T) cells are crucial for the maternal-fetal immune tolerance required a healthy pregnancy outcome. However, their molecular and functional characteristics not well elucidated. In this study, we performed first analysis of transcriptional alternative splicing (AS) landscapes paired decidual peripheral blood (pCD4 in human early using high throughput mRNA sequencing. Our data showed that dCD4 endowed with unique signature when compared to pCD4 cells: upregulate 1,695...
CD40-CD40L and inducible co-stimulatory molecule (ICOS)-ICOSL ligations are demonstrated to play critical roles in CD4+ T-B interaction for B cell activation differentiation mouse models. Herein, by using a micropipette adhesion assay an vitro coculture system simultaneously, we intended dissect their human IgG/IgM production. With the upregulation of CD40L ICOS expressions on T cells upon TCR/CD28 stimulation vitro, activated exhibited enhanced with autologous at single level when compared...
Polybrominated diphenyl ethers (PBDEs) are ubiquitous environmental pollutants that accumulate to high levels in human populations subject occupational or regional industry exposure. PBDEs have been shown affect neuronal, endocrine and reproductive systems, but their effect on the immune system is not well understood. In this study, experimental adult mice were intragastrically administered 2,2′,3,3′,4,4′,5,5′,6,6′-decabromodiphenyl ether (BDE-209) at doses of 8, 80 800 mg/kg body weight...
Abstract A deeper understanding of the immunological events during pregnancy will provide novel insights into pathogenesis complications. The fundamental function T follicular helper (Tfh) cells is to cognate help B cells. Dysregulations Tfh-cell and/or development can result in various diseases. However, role and characteristics Tfh remain unknown. Herein, an allogeneic-normal-pregnant mouse model was used, we found that CD4 + residing at uterus placenta (UP) displayed a Tfh-like phenotype;...
Psoriasis is a chronic inflammatory skin disorder that impairs the quality of life affected patients. Emerging studies indicate certain long non-coding RNAs (lncRNAs) have important roles in psoriasis. However, exact functions lncRNAs and their regulatory mechanisms as competitive endogenous (ceRNAs) psoriasis remained to be fully elucidated. In present study, differentially expressed lncRNAs, microRNAs (miRNAs) mRNAs were identified by analyzing public datasets, psoriasis-associated...
Abstract Systemic Lupus Erythematosus (SLE) and pemphigus are two representative autoimmune diseases driven by pathogenic autoantibody systemically organ-specifically, respectively. Given the involvement of antibody in pathogenesis, B cells inclined to differentiate function an abnormal activation model. Here we defined a unique CD19 hi cell population existing periphery SLE patients as well human tonsils. could be induced vitro after co-culturing fully activated CD4 + T with autologous...
Decidual CD8+ (dCD8) T cells have been proposed to play important roles in immune protection against the invading pathogens and tolerance toward growing semi-allogeneic fetus during early pregnancy. However, their phenotypic functional characteristics remain poorly defined. Here, we performed first analysis of transcriptional alternative splicing (AS) signatures for human first-trimester dCD8 using high-throughput mRNA sequencing. Our data revealed that distinct AS landscapes as compared...
Abstract Joubert syndrome (JS) is a rare clinically and genetically heterogeneous disease. Using whole or targeted exome sequencing, we identified four novel compound heterozygous mutations in chromosome 5 open reading frame 42 gene ( C5orf42 ), including c.2876C>T (missense mutation) c.3921+1G>A (splicing mutation), c.2292 ‐2delA c.4067C>T c.6997_6998insT (frameshift c.8710C>T (nonsense c.3981G>C c.230 _233del Chinese JS families. They were all inherited from their...
Chronic viral infections cause T cell dysfunction in both animal models and human clinical settings, thereby affecting the ability of host immune system to clear pathogens develop proper virus-specific memory. However, impact chronic on host’s memory other has not been well described. In this study, we immunized mice with recombinant Listeria monocytogenes expressing OVA (Lm-OVA) generate immunity Lm allow analysis OVA-specific (Tm) cells. We then infected these lymphocytic choriomeningitis...
Abstract Preterm labor/birth is the leading cause of perinatal mortality and morbidity worldwide. Previous studies demonstrated that T cells were crucial for maintaining maternal–fetal immune tolerance during first trimester pregnancy; however, their phenotypes functions in labor delivery remain largely unknown. We recruited three cohorts women at T‐cell immunophenotyping placentas, fetal membranes, umbilical cord blood, maternal peripheral blood. Our data showed a differential enrichment...
Objective To investigate the activation state of and expressions surface co-stimulatory molecules on peripheral CD4+ T cells from patients with pemphigus healthy human controls.Methods Ninety including 24 first-onset pemphigus,51 quiescent 15 recurrent pemphigus,as well as 30 controls were enrolled in this study.Peripheral blood samples obtained these subjects followed by lymphocyte isolation.Flow cytometry was performed to detect CD69,intercellular adhesion molecule-1 (ICAM-1),inducible...
Objective To investigate pathological features of infiltrating lymphocytes in skin lesions patients with pemphigus, and to analyze their correlation titers anti-desmoglein (Dsg) 1 anti-Dsg3 antibodies peripheral blood. Methods A retrospective analysis was performed 93 pemphigus vulgaris or foliaceus, who visited the Department Dermatology Ruijin Hospital affiliated Shanghai Jiao Tong University School Medicine between 2014 2016. For each HE-stained section, total number per ×50...
Objective To evaluate the specific antibody-producing capacity of locally infiltrating B lymphocytes in lesions patients with pemphigus vulgaris (PV) . Methods Totally, 35 PV and 22 healthy controls were enrolled into this study. Skin tissues resected from blisters or erosions PV, normal skin controls. Then, mononuclear cells isolated these tissues. Flow cytometry was performed to determine percentages lymphocytes, CD19+ desmoglein (Dsg) 1- Dsg3-specific lymphocytes. lesional cultured...
Objective To compare the diagnostic value of fluorescent staining versus KOH wet-mount microscopy in detecting superficial fungal infection. Methods Totally, 600 specimens from cases clinically diagnosed infections and 102 Malassezia infection (including 54 pityriasis versicolor 48 folliculitis) were collected dermatology clinic Tenth People′s Hospital Tongji University between July 2017 February 2018. These subjected to wet mount separately followed by direct microscopy, positive...