A5065905821- Epigenetics and DNA Methylation
- Gut microbiota and health
- Fibroblast Growth Factor Research
- Diet and metabolism studies
- Genomics and Chromatin Dynamics
- Cancer, Hypoxia, and Metabolism
- Trypanosoma species research and implications
- Metabolism and Genetic Disorders
- Erythropoietin and Anemia Treatment
- Neonatal Respiratory Health Research
- Biochemical and Molecular Research
- Cancer-related Molecular Pathways
- Congenital Diaphragmatic Hernia Studies
- Medical Imaging and Pathology Studies
- RNA modifications and cancer
- Blood transfusion and management
- Research on Leishmaniasis Studies
- Oral Health Pathology and Treatment
- RNA Research and Splicing
- Neuroinflammation and Neurodegeneration Mechanisms
- Hedgehog Signaling Pathway Studies
- Cancer-related gene regulation
- Adipose Tissue and Metabolism
- Digestive system and related health
- Telomeres, Telomerase, and Senescence
University of Pennsylvania
2018-2024
Case Western Reserve University
2024
University School
2024
Washington University in St. Louis
2023
Instituto Butantan
2016-2023
Universidade de São Paulo
2016
Secretaria da Educação
2016
Premature birth disrupts normal lung development and places infants at risk for bronchopulmonary dysplasia (BPD), a disease disrupting health throughout the life of an individual that is increasing in incidence. The TGF-β superfamily has been implicated BPD pathogenesis, however, what cell lineage it impacts remains unclear. We show TGFbr2 critical alveolar epithelial (AT1) fate maintenance function. Loss AT1 cells during late leads to AT1-AT2 reprogramming altered pulmonary architecture,...
DNA and histone proteins define the structure composition of chromatin. Histone posttranslational modifications (PTMs) are covalent chemical groups capable modeling chromatin accessibility, mostly due to their ability in recruiting enzymes responsible for readout remodeling. Mass spectrometry (MS)-based proteomics is methodology choice large-scale identification quantification protein PTMs, including histones. High sensitivity requires online MS coupling with relatively low throughput poorly...
Histones are well-conserved proteins that form the basic structure of chromatin in eukaryotes and undergo several post-translational modifications, which important for control transcription, replication, DNA damage repair, chromosome condensation. In early branched organisms, histones less conserved appear to contain alternative sites could reveal evolutionary unique functions histone modifications gene expression other chromatin-based processes. Here, by using high-resolution mass...
Abstract Histone post-translational modifications (PTMs) contribute to chromatin accessibility due their chemical properties and ability recruit enzymes responsible for DNA readout remodeling. To date, more than 400 different histone PTMs thousands of combinations have been identified, the vast majority with still unknown biological function. Identification quantification has become routine in mass spectrometry (MS) but, since raising antibodies each PTM a study can be prohibitive, lots...
Chromatin associated proteins are key regulators of many important processes in the cell. Trypanosoma cruzi, a protozoa flagellate that causes Chagas disease, alternates between replicative and nonreplicative forms accompanied by shift on global transcription levels changes its chromatin architecture. Here, we investigated T. cruzi proteome using three different protocols compared it (epimastigote) (trypomastigote) high-resolution mass spectrometry. More than 2000 were identified quantified...
The eukaryotic genome is packaged around histone proteins, which are subject to a myriad of post-translational modifications. By controlling DNA accessibility and the recruitment protein complexes that mediate chromatin-related processes, these modifications constitute key mechanism epigenetic regulation. Since mass spectrometry can easily distinguish between different modifications, it has become an essential technique in deciphering code. Although robust LC–MS/MS methods available analyze...
Abstract Fibroblast growth factor 2 (FGF2) is a well‐known cell proliferation promoter; however, it can also induce cycle arrest. To gain insight into the molecular mechanisms of this antiproliferative effect, for first time, early systemic proteomic differences induced by in K‐Ras‐driven mouse tumor line using quantitative proteomics approach are investigated. More than 2900 proteins quantified, indicating that terms associated with metabolism, RNA processing, replication, and transcription...
The nucleolus is sensitive to stress and can orchestrate a chain of cellular events in response signals. Despite being growth factor, FGF2 has antiproliferative tumor-suppressive functions some contexts. In this work, we investigated how the effect modulates chromatin-, nucleolus- rDNA-associated proteins. chromatin nucleolar proteome indicated that stimulation proteins related transcription, rRNA expression chromatin-remodeling global transcriptional rate area increased along with...
ABSTRACT The eukaryotic genome is packaged around histone proteins, which are subject to a myriad of post-translational modifications. By controlling DNA accessibility and the recruitment protein complexes that mediate chromatin-related processes, these modifications constitute key mechanism epigenetic regulation. Since mass spectrometry can easily distinguish between different modifications, it has become an essential technique in deciphering code. Although robust LC-MS/MS methods available...
The gut microbiota influences host epigenetics by fermenting dietary fiber into butyrate. Although butyrate could promote histone acetylation inhibiting deacetylases, it may also undergo oxidation to acetyl-CoA, a necessary cofactor for acetyltransferases. Here, we find that epithelial cells from germ-free mice harbor loss of H4 across the genome except at promoter regions. Using stable isotope tracing in vivo with 13C-labeled fiber, demonstrate supplies carbon acetylation. Subsequent...
ABSTRACT Premature birth disrupts normal lung development and places infants at risk for bronchopulmonary dysplasia (BPD), a disease increasing in incidence which health throughout the lifespan. The TGFβ superfamily has been implicated BPD pathogenesis, however, what cell lineage it impacts remains unclear. We show that Tgfbr2 is critical AT1 fate maintenance function. Loss of cells during late leads to AT1-AT2 reprogramming altered pulmonary architecture, persists into adulthood....
Paradoxically, oncogenes that drive cell cycle progression may also trigger pathways leading to senescence, thereby inhibiting the growth of tumorigenic cells. Knowledge how these operate, and tumor cells evade pathways, is important for understanding tumorigenesis. The Y1 line, which harbors an amplification proto-oncogene Ras, rapidly senesces in response mitogen fibroblast factor-2 (FGF-2). To gain a more complete picture FGF-2 promotes we employed multi-omics approach analyze histone...
Background: In conditions of hyperactive cellular metabolism, excessive nutrient uptake results in incomplete metabolism and excretion intermediates. These intermediates may serve as unconventional fuel sources satisfy metabolic demands during scarcity. Interestingly, acetate provides a parallel pathway for acetyl-CoA production allows protein acetylation lipogenesis independent citrate conversion to acetyl-CoA. This is important tumorigenesis, immune alertness, neural plasticity, other...
Abstract Background: In conditions of hyperactive cellular metabolism, excessive nutrient uptake results in incomplete metabolism and excretion intermediates. These intermediates may serve as unconventional fuel sources satisfy metabolic demands during scarcity. Interestingly, acetate provides a parallel pathway for acetyl-CoA production allows protein acetylation lipogenesis independent citrate conversion to acetyl-CoA. This is important tumorigenesis, immune alertness, neural plasticity,...
ABSTRACT Paradoxically, oncogenes that drive cell cycle progression may also trigger pathways leading to senescence, thereby inhibiting the growth of tumorigenic cells. Along these lines, Y1 cells, which carry an amplification Ras, become senescent after treatment with mitogen FGF-2. To understand how FGF-2 promotes we profiled epigenome, transcriptome, proteome, and phospho-proteome cells stimulated caused delayed acetylation histone H4 higher levels H3K27me3. Sequencing analysis revealed...
Abstract Inflammatory bowel disease (IBD) is often associated with a disruption in the composition and activity of gut microbiota, referred to as dysbiosis. Since microbiota has potential interact host epithelial cells through small molecules generated from microbial metabolism, knowledge how inflammation alters metabolome react important for better understanding IBD develops persists. Butyrate, short chain fatty acid produced fermentation dietary polysaccharides, long been known inhibit...