Protein-coding genes are considered to be a dominant component of the eukaryotic transcriptome; however, many studies have shown that intergenic, non-coding transcripts also play an important role. Long intergenic RNAs (lincRNAs) were found vital role in human and Arabidopsis. However, lincRNAs their regulatory roles remain poorly characterized woody plants, especially Populus trichocarpa (P. trichocarpa). A large set RNA-Seq data examined with high sequencing depth under control drought...

Drought, a primary abiotic stress, seriously affects plant growth and productivity. Stomata play vital role in regulating gas exchange drought adaptation. However, limited knowledge exists of the molecular mechanisms underlying stomatal movement trees. Here, PeCHYR1, ubiquitin E3 ligase, was isolated from Populus euphratica, model stress adaptation forest PeCHYR1 preferentially expressed young leaves significantly induced by ABA (abscisic acid) dehydration treatments. To study potential...

Abstract Lotus, an economically significant aquatic crop within the genus Nelumbo (family naceae), includes two extant species nucifera and lutea . Renowned for its ornamental, culinary, medicinal value, lotus has seen notable advancements in genomics molecular biology, particularly reference genome sequencing assembly, bioactive compound biosynthetic pathways, mechanisms underlying key traits. Critical genes linked to horticultural characteristics, edible quality, properties, stress...

Summary In the present study, Pe STZ 1 , a cysteine‐2/histidine‐2‐type zinc finger transcription factor, was isolated from desert poplar, Populus euphratica which serves as model stress adaptation system for trees. preferentially expressed in young stems and significantly up‐regulated during chilling freezing treatments. localized to nucleus bound specifically APX 2 promoter. To examine potential functions of we overexpressed it poplar 84K hybrids ( alba × glandulosa ), are known be...

Succinate dehydrogenase (SDH) has been identified as one of the most significant targets for fungicide discovery. To date, 23 commercial SDH inhibitor (SDHI) fungicides have approved plant protection since first launch carboxin in 1966, and extensively applied to combat destructive fungi.In this project, 20 novel pyridine sulfide derivatives containing SDH-based heterocyclic amide were designed, synthesized, characterized by proton nuclear magnetic resonance (1 H-NMR), carbon-13 (13 C)-NMR...

Water availability is a main limiting factor for plant growth, development, and distribution throughout the world. Stomatal movement mediated by abscisic acid (ABA) particularly important drought adaptation, but molecular mechanisms in trees are largely unclear. Here, we isolated an ABA-responsive element binding factor, PeABF3, Populus euphratica. PeABF3 was preferentially expressed xylem young leaves, induced dehydration ABA treatments. showed transactivation activity located nucleus. To...

The hemibiotroph Colletotrichum gloeosporioides and the necrotroph Cytospora chrysosperma cause poplar foliage stem disease, respectively, resulting in substantial economic losses. In this study, Populus trichocarpa ptc-miR472a was down-regulated leaves treated with salicylic acid, jasmonic acid (JA) or bacterial flagellin (flg22). Here, a short tandem target mimic (STTM) of miR472a were overexpressed P. alba × glandulosa, overexpression lines silenced STTM472a generated. Compared wild type...

Abstract ZINC FINGER OF ARABIDOPSIS THALIANA12 (ZAT12) plays an important role in stress responses, but the transcriptional regulation of ZAT12 response to abiotic remains unclear. In this study, we confirmed that a SALT TOLERANCE FINGER1 transcription factor from Populus euphratica (PeSTZ1) could regulate expression PeZAT12 by dual-luciferase reporter (DLR) assay and electrophoretic mobility shift assay. The PeSTZ1 was rapidly induced NaCl hydrogen peroxide (H2O2) treatments. Overexpressing...

Poplar is one of the most important tree species in north temperate zone, but poplar plantations are quite water intensive. We report here that CaMV 35S promoter-driven overexpression PdERECTA gene, which a member LRR-RLKs family from Populus nigra × (Populus deltoides nigra), improves use efficiency and enhances drought tolerance triploid white poplar. localizes to plasma membrane. Overexpression plants showed lower stomatal density larger size. The abaxial was 24–34% size 12–14% lines....

Sodium salicylate (NaSal), an aspirin metabolite, can cause tinnitus in animals and human subjects. To explore neural mechanisms underlying salicylate-induced tinnitus, we examined effects of NaSal on activities the medial geniculate body (MGB), auditory thalamic nucleus that provides primary immediate inputs to cortex, by using whole-cell patch-clamp recording technique MGB slices. Rats treated with (350 mg/kg) showed tinnitus-like behavior as revealed gap prepulse inhibition acoustic...

Despite the unshakable status of reverse transcription-quantitative PCR in gene expression analysis, it has certain disadvantages, including that results are highly dependent on reference genes selected for data normalization. Since inappropriate endogenous control will lead to inaccurate target profiles, validation suitable internal is essential. Given increasing interest functional and genomics Populus euphratica, a desert poplar showing extraordinary adaptation salt stress, we evaluated...

Abstract Understanding the impact of tillage erosion on soil organic carbon (SOC) and nitrogen (N) fractions is essential for targeted conservation in mountainous hilly areas. However, little known about this issue. In study, we selected a erosion-dominated hillslope from Sichuan Basin, China, to determine effect particulate OC (POC), dissolved (DOC), light fraction (LFOC), ammonium N (NH 4 + -N), nitrate (NO 3 − -N) alkali-hydrolysable (AN). Additionally, investigated microbial activities...

F-box proteins are important components of eukaryotic SCF E3 ubiquitin ligase complexes, which specifically determine protein substrate proteasomal degradation during plant growth and development, as well biotic abiotic stress. It has been found that the FBA (F-box associated) family is one largest subgroups widely prevalent plays significant roles in development stress response. However, gene poplar not systematically studied to date. In this study, a total 337 candidate genes were...

Drought is a major environmental factor limiting the production of crops. tolerance sugar beet (Beta vulgaris L.) cultivars in terms growth and inorganic organic solute accumulation was analyzed this study. Four-week-old seedlings three cultivars, “Gantang7”, “SD13829” “ST21916”, differing drought tolerance, were treated with different degrees stress. Plant shoots roots harvested at 5 d after stress subjected to analysis. significantly reduced shoot fresh weight water content though least...

Distinguishing the five types of Fritillariae bulbus (Fritillariae cirrhosae bulbus, ussuriensis pallidiflorae thunbergii and hupehensis bulbus) is difficult because their similar morphological, physical, chemical characteristics. These species, which are included in Chinese Pharmacopoeia, exhibit different therapeutic effects. Therefore, we used internal transcribed spacer 2 (ITS2) regions as DNA barcode to distinguish from each other adulterants. A total 151 samples belonging (a 11 species...

Folium Artemisiae Argyi is an important herb in traditional Chinese medicine. It commonly used moxibustion, medicine, etc. However, identifying Artemisia argyi difficult because this exhibits similar morphological characteristics to closely related species and counterfeits. To verify the applicability of DNA barcoding, ITS2 psbA-trnH were identify A. from 15 Results indicated that total was easily extracted all samples both fragments can be amplified. a more ideal barcode than ITS2+psbA-trnH...

NAC (NAM, ATAF, and CUC) is a ubiquitously expressed plant-specific transcription factor (TF) family which involved in the regulation of various biological processes. However, systematic characterization gene yet to be reported lotus. Here, 82 NnNAC genes included five predicted membrane-bound proteins were identified lotus genome. Phylogenetic analysis revealed seven-subfamily clusters (I–VII) proteins, with homologous pairs displaying similar conserved motifs structure characteristics....

Abstract Artemisia annua is the only known plant source of potent antimalarial artemisinin, which occurs as low- and high-artemisinin producing (LAP HAP) chemotypes. Nevertheless, different mechanisms artemisinin between these two chemotypes were still not fully understood. Here, we performed a comprehensive analysis genome resequencing, metabolome, transcriptome data to systematically compare difference in LAP chemotype JL HAP HAN. Metabolites revealed that 72.18% sesquiterpenes was highly...