A5074030558- Bone Metabolism and Diseases
- Osteoarthritis Treatment and Mechanisms
- Bone health and treatments
- Spine and Intervertebral Disc Pathology
- Bone health and osteoporosis research
- Orthopaedic implants and arthroplasty
- Periodontal Regeneration and Treatments
- Sirtuins and Resveratrol in Medicine
- Bone Tissue Engineering Materials
- Veterinary Orthopedics and Neurology
- TGF-β signaling in diseases
- Bone and Dental Protein Studies
- Total Knee Arthroplasty Outcomes
- Adipose Tissue and Metabolism
- Parathyroid Disorders and Treatments
- Dental Implant Techniques and Outcomes
- Orthopedic Infections and Treatments
- Mesenchymal stem cell research
- Muscle metabolism and nutrition
- Lower Extremity Biomechanics and Pathologies
- Vitamin D Research Studies
- Electrolyte and hormonal disorders
- Bone fractures and treatments
- Adipokines, Inflammation, and Metabolic Diseases
- Axon Guidance and Neuronal Signaling
Tokyo Medical and Dental University
2014-2023
The University of Tokyo
2023
Shanghai University
2023
Tokyo Medical and Dental University Hospital
2015
Wayne State University
2014
Neurological Surgery
2014
Olympus (Japan)
2014
China Medical University
2014
First Hospital of China Medical University
2014
Capital Medical University
2014
Abstract Osteocytes are major bone cells that play a crucial role in maintaining the quality of and healing damage to tissue. The number living osteocytes canalicular networks declines an age-dependent manner. However, pathological effects mitochondrial redox imbalances on metabolism have not been fully elucidated. We generated mice lacking superoxide dismutase 2 ( Sod2 ) osteocytes. Like aged bone, depletion positively enhanced production cellular vivo . A morphological analysis...
Osteoclastic bone resorption requires a number of complex steps that are under the control local regulatory molecules. Osteopontin is expressed in osteoclasts and also present matrix; however, its biological function has not been fully understood. To elucidate role osteopontin process osteoclastic resorption, we conducted ectopic implantation experiments using wild-type knockout mouse. In group, discs from calvariae implanted ectopically muscle were resorbed, their mass was reduced by 25%...
Embryonic development of tendons is in close association with that cartilage and bone. Although these tissues are derived from mesenchymal progenitor cells which also give rise to muscle fat, their fates clearly diverse early embryonic stages. Transcription factors may play pivotal roles the process determination differentiation tendon as well other skeletal system. Scleraxis, a basic helix-loop-helix (bHLH) type transcription factor, expressed progenitors later form connective including...
Obesity and high body mass index are associated with a higher incidence of osteoarthritis (OA). The aim this study is to investigate the involvement infrapatellar fat pad (IPFP) in sub-acute effect diet (HFD) on development knee-OA. C57BL/6J male mice were fed either HFD or normal beginning at seven weeks age. Tissue sections evaluated immunohistological analysis. IPFP was excised, mRNA expression profiles compared using real-time RT-PCR Osteoarthritic changes initiated group after eight...
ABSTRACT Oxidative stress contributes to the pathogenesis of age-related diseases as well bone fragility. Our previous study demonstrated that copper/zinc superoxide dismutase (Sod1)-deficient mice exhibit induction intracellular reactive oxygen species (ROS) and fragility resulting from low-turnover loss impaired collagen cross-linking (Nojiri et al. J Bone Miner Res. 2011;26:2682–94). Mechanical also plays an important role in maintenance homeostasis tissue. However, molecular links...
Objective and Background Periodontitis is an inflammatory disorder of the supporting tissue teeth, which composed gingival soft tissue, cementum covering tooth root, alveolar bone periodontal ligament. The receptor activator nuclear factor kappa B ligand ( RANKL ) known to be essential for osteoclastogenesis. Recent clinical studies indicate that levels in crevicular fluid are increased while its decoy receptor, osteoprotegerin OPG ), decreased patients with periodontitis. Although sulcus...
Osteoblastic differentiation is regulated by various factors, including hormones and transcription factors. Runt‐related factor 2 (Runx2) an essential player in osteoblastogenesis transactivates its molecular target creating a protein complex with hetero‐dimeric partner core binding beta (Cbfb). However, the regulation of Cbfb expression remains unknown. Here, we identified miR‐145 as crucial regulator expression. The increased during osteoblastogenesis, indicating that works inhibitor...
Abstract Objective To investigate the effect of osteoclastogenesis inhibitory factor/osteoprotegerin (OPG) on chondrocytes in development osteoarthritis (OA) vivo. Methods determine role endogenous OPG progression OA, OA was surgically induced +/− mice and their wild‐type (WT) littermates. exogenous OPG, knee joints C57BL/6J with were injected intraarticularly recombinant human (rHuOPG) or vehicle 5 times a week. All euthanized 4 weeks after induction; harvested evaluated...
Intervertebral disc degeneration (IVDD) greatly affects the quality of life. The nucleus pulposus (NP) chondrodystrophic dog breeds (CDBs) is similar to human NP because cells disappear with age and are replaced by fibrochondrocyte‐like cells. Because IVDD develops as early within first year life, we used canines a model investigate in vitro mechanisms underlying IVDD. mechanism age‐related IVDD, however, poorly understood. Several research groups have suggested that Wnt/β‐catenin signaling...
Abstract Objective Runx2 is a transcription factor that regulates chondrocyte differentiation. This study was undertaken to address the role of different Runx proteins (Runx1, Runx2, or Runx3) in differentiation using chondrocyte‐specific –transgenic mice, and importance QA domain which involved its transcriptional activation. Methods expression analyzed mouse embryo by situ hybridization. Overexpression 1, 2 (lacking [ΔQA]), 3 induced chondrocytes vivo, produce α(1)II‐ 2ΔQA, respectively,...
Abstract Sirtuin 6 (Sirt6) is a mammalian homologue of NAD + -dependent histone deacetylase Sir2. Although Sirt6−/− mice exhibit growth retardation, the role Sirt6 in cartilage metabolism unclear. The aim this study was to investigate signaling pathway metabolism. Immunohistological evaluation tibial plate exhibited impaired proliferation and differentiation chondrocytes, reduced expression Indian hedgehog (Ihh) senescent phenotype. When knocked down chondrocytes vitro, Ihh its downstream...
Abstract The purpose of the present study was to confirm if proanthocyanidin-rich grape seed extract (GSE) had ability improve bone health such as loss, healing, and implant osseointegration (defined direct connection between tissue an implant) in ovariectomized (OVX) animals. We demonstrated that daily oral administration GSE prevented loss lumbar vertebrae femur OVX mice. In addition, osteoclastogenesis spine mice, assessed by histological histomorphometric analyses, accelerated but this...
To investigate the molecular mechanisms underlying particle-induced osteolysis, we focused on osteopontin (OPN), a cytokine and cell-attachment protein that is associated with macrophage chemoattractant osteoclast activation.We compared OPN levels in human periprosthetic osteolysis tissues those osteoarthritis (OA) synovial tissues. functions of during vivo, titanium particles were implanted onto calvaria OPN-deficient mice their wild-type (WT) littermates. Mice killed day 10 evaluated...
Osteoarthritis (OA) is a common disease in the elderly due to an imbalance cartilage degradation and synthesis. Heterotopic ossification (HO) occurs when ectopic masses of endochondral bone form within soft tissues around joints triggered by inflammation tissues. Procyanidin B3 (B3) procyanidin dimer that widely studied its high abundance human diet antioxidant activity. Here, we evaluated role isolated from grape seeds maintenance chondrocytes vitro vivo. We observed inhibited H2O2-induced...
Grape-seed extract (GSE) is rich in proanthocyanidins (polymers of flavan-3-ols). GSE well known to have various beneficial effects health. The objective this study was examine the effect dietary on intestinal microbiota ovariectomized (OVX) mice as a model menopause. Phylum-level analyses using 16S rRNA-targeted group-specific polymerase-chain reaction primers fecal samples collected 8 weeks postoperatively from OVX revealed that proportion Firmicutes and Bacteroidetes populations became...
Osteoarthritis (OA) is a chronic degenerative joint disorder commonly associated with metabolic syndrome. As ageing and obesity has great impact on the initiation/severity of OA, herein we sought to investigate involvement Sirt6 in crosstalk between syndrome/OA. haploinsufficiency mice promoted expression inflammatory cytokines IPFP. Enhanced inflammation IPFP aged ± HFD group was paralleled accelerated OA change, including osteophyte growth chondrocyte hypertrophy. Conversely,...
Osteoclastic bone resorption requires a number of complex steps that are under the control local regulatory molecules. Osteopontin is expressed in osteoclasts and also present matrix; however, its biological function has not been fully understood. To elucidate role osteopontin process osteoclastic resorption, we conducted ectopic implantation experiments using wild-type knockout mouse. In group, discs from calvariae implanted ectopically muscle were resorbed, their mass was reduced by 25%...
To evaluate effects of long-term administration carprofen on healing a tibial osteotomy in dogs.12 healthy female Beagles.A mid-diaphyseal transverse (stabilized with an intramedullary pin) the right tibia was performed each dog. The group (n = 6 dogs) received (2.2 mg/kg, PO, q 12 h) for 120 days; control (6) no treatment. Bone and change callus area were assessed radiographically over time. Dogs euthanized days after surgery, tibiae evaluated biomechanically histologically.The line not...
Intervertebral disc (IVD) degeneration greatly affects quality of life. The nucleus pulposus (NP) chondrodystrophic dog breeds (CDBs) is similar to the human NP, because cells disappear with age and are replaced by fibrochondrocyte-like cells. However, IVD develops as early within first year life, we used canines a model investigate in vitro mechanisms underlying degeneration. Specifically, evaluated potential three-dimensional (3D) culture healthy NP an system Agarose hydrogels were...
Obesity is a risk factor for osteoarthritis (OA). To investigate the roles of increased mechanical loading in onset obesity-induced OA, knee joints were histologically analyzed after applying tail suspension (TS) model to high-fat diet (HFD)-induced OA model. Mice divided into four groups: normal (ND) with (NL) group; HFD NL ND TS and group. Whole evaluated by immunohistological analysis. The infrapatellar fat pad (IPFP) was excised mRNA expression profiles compared qPCR After twelve weeks...