A5090966781- Iron Metabolism and Disorders
- Trace Elements in Health
- Hemoglobinopathies and Related Disorders
- Ferroptosis and cancer prognosis
- Extracellular vesicles in disease
- RNA regulation and disease
- Heme Oxygenase-1 and Carbon Monoxide
- Reproductive tract infections research
- Autophagy in Disease and Therapy
- Hedgehog Signaling Pathway Studies
- Porphyrin Metabolism and Disorders
- Occupational and environmental lung diseases
- Genetic and Kidney Cyst Diseases
- Endoplasmic Reticulum Stress and Disease
- Microtubule and mitosis dynamics
- Cellular transport and secretion
- Genetic and rare skin diseases.
- Clostridium difficile and Clostridium perfringens research
- Biomarkers in Disease Mechanisms
- Syphilis Diagnosis and Treatment
- Cervical Cancer and HPV Research
- Glutathione Transferases and Polymorphisms
- Mitochondrial Function and Pathology
- Fungal and yeast genetics research
- Neurological diseases and metabolism
Nagoya University
2019-2024
Kyoto University
2023-2024
Kawasaki Medical School
2009-2021
Stanford University
2018-2021
Harvard University Press
2013
AIMM Therapeutics (Netherlands)
2013
Parkinson's disease-associated LRRK2 kinase phosphorylates multiple Rab GTPases, including Rab8A and Rab10. We show here that interferes with primary cilia formation in cultured cells, human G2019S iPS cells the cortex of R1441C mice. Rab10 phosphorylation strengthens its intrinsic ability to block ciliogenesis by enhancing binding RILPL1. Importantly, interfere requires both RILPL1 proteins. Pathogenic influences respond cilia-dependent, Hedgehog signaling as monitored Gli1 transcriptional...
Endosomal recycling of the mammalian iron transporter DMT1 is assumed to be important for efficient and rapid uptake across endosomal membrane in transferrin cycle. Here, we show that retromer, a complex mediates retrograde transport transmembrane cargoes from endosomes trans-Golgi network, required DMT1-II, an alternative splicing isoform DMT1. Bacterially expressed Vps26-Vsp29-Vsp35 trimer, retromer cargo recognition complex, specifically binds cytoplasmic tail domain DMT1-II vitro. In...
DMT1 (divalent metal transporter 1) is the main iron importer found in animals, and ferrous taken up by cells via DMT1. Once reaches cytosol, it subjected to subcellular distribution delivered various sites where required for a variety of biochemical reactions cell. Until now, mechanism connecting cytosolic had not been clarified. In present study, we have identified PCBP2 [poly(rC)-binding protein 2] as DMT1-binding protein. The N-terminal cytoplasmic region binding domain PCBP2. An...
Pancreatic ductal adenocarcinoma (PDAC) is characterized by an extensive desmoplastic stromal response. Fibroblast activation protein-α (FAP) best known for its presence in cancer-associated fibroblasts (CAFs). Our aim was to assess whether FAP expression associated with the prognosis of patients PDAC and investigate how expressing CAFs contribute progression PDAC. immunohistochemically assessed 48 specimens. We also generated a fibroblastic cell line stably FAP, examined effect...
Asbestos-associated diseases remain a social burden worldwide. Our previous studies identified asbestos-induced iron-rich milieu for mesothelial cells with ceaseless macrophage ferroptosis. However, molecular mechanisms how this mutagenic influences have not been elucidated yet. Here, we propose novel mechanism that extracellular vesicles (EVs) mediate asbestos-associated factors to cells. In mice model of intraperitoneal crocidolite injection, highly expressed CD63, an exosomal marker. We...
Activating mutations in LRRK2 kinase causes Parkinson’s disease. Pathogenic phosphorylates a subset of Rab GTPases and blocks ciliogenesis. Thus, defining novel phospho-Rab interacting partners is critical to our understanding the molecular basis pathogenesis. RILPL2 binds with strong preference LRRK2-phosphorylated Rab8A Rab10. binding partner motor protein effector, Myosin Va. We show here that globular tail domain Va also contains high affinity site for In presence pathogenic LRRK2, MyoVa...
Abstract Background Heme and non-heme iron from diet, recycled hemoglobin are important products of the synthesis iron-containing molecules. In excess, is potentially toxic because it can produce reactive oxygen species through Fenton reaction. Humans absorb, transport, store, recycle without an excretory system to remove excess iron. Two candidate heme transporters two have been reported thus far. incorporated into cells degraded by oxygenases (HOs), product reutilized body. To specify...
Mammals incorporate a major proportion of absorbed iron as heme, which is catabolized by the heme oxygenase 1 (HO1)-NADPH-cytochrome P450 reductase (CPR) complex into biliverdin, carbon monoxide, and ferrous iron. Moreover, intestinal incorporated iron, transported via importer, divalent metal transporter (DMT1). Recently, we demonstrated that chaperone poly(rC)-binding protein 2 (PCBP2) can directly receive from DMT1 or transfer to exporter, ferroportin 1. To promote intracellular flux, an...
Asbestos is still a social burden worldwide as carcinogen causing malignant mesothelioma. Whereas recent studies suggest that local iron reduction preventive strategy against carcinogenesis, little known regarding the cellular and molecular mechanisms surrounding excess iron. Here by differentially using high-risk low-risk asbestos fibers (crocidolite anthophyllite, respectively), we identified asbestos-induced mutagenic milieu for mesothelial cells. Rat cell experiments revealed...
Abstract Organoids are a three-dimensional (3D) culture system that simulate actual organs. Therefore, tumor organoids expected to predict precise response chemotherapy in patients. However, date, few studies have studied the drug responses of malignant mesothelioma (MM). The poor prognosis MM emphasizes importance establishing protocol for generating MM-organoid research and clinical use. Here, we established murine from p53 +/- or wild-type C57BL/6 strain by intraperitoneal injection...
Current progress in biology and medical science is based on the observation at level of nanometers via electron microscopy computation. Of note, size most cells higher species exists a limited range from 5 to 50 μm. Recently, it was demonstrated that endogenous extracellular nanoparticles play role communication among various cellular types variety contexts. Among them, exosomes serum have been established as biomarkers for human diseases by analyzing cargo molecules. No life earth can...