A5057629182- Cancer Cells and Metastasis
- Chemokine receptors and signaling
- Neuroblastoma Research and Treatments
- Neuroendocrine Tumor Research Advances
- Glioma Diagnosis and Treatment
- Histone Deacetylase Inhibitors Research
- Virus-based gene therapy research
- Ovarian cancer diagnosis and treatment
- Metabolism, Diabetes, and Cancer
- Occupational and environmental lung diseases
- Immunotherapy and Immune Responses
- Lung Cancer Research Studies
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Pleural and Pulmonary Diseases
- Endometriosis Research and Treatment
- Intraperitoneal and Appendiceal Malignancies
- Pituitary Gland Disorders and Treatments
- Advanced Biosensing Techniques and Applications
- Natural Compounds in Disease Treatment
- Animal Genetics and Reproduction
- Prion Diseases and Protein Misfolding
- Meningioma and schwannoma management
- Spaceflight effects on biology
- Plant chemical constituents analysis
- Ear and Head Tumors
University of Genoa
2006-2020
Alleanza Contro il Cancro
2010
Torino e-district
1987
Istituto Nazionale di Fisica Nucleare, Sezione di Genova
1987
Cancer stem cell theory postulates that a small population of tumor-initiating cells is responsible for the development, progression and recurrence several malignancies, including glioblastoma. In this perspective, represent most relevant target to obtain effective cancer treatment. Metformin, first-line drug type II diabetes, was reported possess anticancer properties affecting survival in breast models. We report metformin treatment reduced proliferation rate cell-enriched cultures...
Peptide receptors involved in pathophysiological processes represent promising therapeutic targets. Neuropeptide somatostatin (SST) is produced by specialized cells a large number of human organs and tissues. SST primarily acts as inhibitor endocrine exocrine secretion via the activation five G-protein-coupled receptors, named sst1–5, while central nervous system, neurotransmitter/neuromodulator, regulating locomotory cognitive functions. Critical points SST/SST receptor biology, such...
Because a subpopulation of cancer stem cells (tumor-initiating cells, TICs) is believed to be responsible for the development, progression, and recurrence many tumors, we evaluated in vitro sensitivity human glioma TICs epidermal growth factor receptor (EGFR) kinase inhibitors (erlotinib gefitinib) possible molecular determinants their effects. Cells isolated from seven glioblastomas (GBM 1-7) grown using neural cell permissive conditions were characterized vivo tumorigenicity, expression...
Glioblastoma (GBM), the most common primary brain tumor in adults, is an aggressive, fast-growing and highly vascularized tumor, characterized by extensive invasiveness local recurrence. In GBM other malignancies, cancer stem cells (CSCs) are believed to drive invasive growth recurrence, being responsible for radio- chemo-therapy resistance. Mesenchymal (MSCs) multipotent progenitors that exhibit tropism microenvironment mediated cytokines, chemokines factors. Initial studies proposed MSCs...
// Alessandro Corsaro 1 , Adriana Bajetto Stefano Thellung 1, 2 Giulia Begani Valentina Villa Mario Nizzari Alessandra Pattarozzi Agnese Solari Monica Gatti Aldo Pagano 3, 4 Roberto Würth Antonio Daga Federica Barbieri Tullio Florio Sezione di Farmacologia, Dipartimento Medicina Interna, University of Genova, Italy Centro Eccellenza per la Ricerca Biomedica (CEBR), 3 Department Experimental Medicine, IRCCS AOU San Martino - IST, Correspondence to: Florio, email: tullio.florio@unige.it...
Neuroblastoma (NB) is a pediatric cancer characterized by remarkable cell heterogeneity within the tumor nodules. Here, we demonstrate that synthesis of pol III-transcribed noncoding (nc) RNA (NDM29) strongly restricts NB development promoting differentiation, drop malignancy processes, and dramatic reduction initiating (TIC) fraction in population. Notably, overexpression NDM29 also confers to malignant cells an unpredicted susceptibility effects antiblastic drugs used therapy. Altogether,...
Here, we present a minimal hybrid ligand/receptor-based pharmacophore model (PM) for CXCR4, chemokine receptor deeply involved in several pathologies, such as HIV infection, rheumatoid arthritis, cancer development/progression, and metastasization. This model, considerably simpler than those thus far proposed this receptor, has been used to search new CXCR4 inhibitors small marine natural product library available at ICB-CNR Institute (Pozzuoli, NA, Italy), since products, with their...
Cancer stem cells (CSCs) are considered the cell subpopulation responsible for breast cancer (BC) initiation, growth, and relapse. CSCs identified as self-renewing tumor-initiating cells, conferring resistance to chemo- radio-therapy several neoplasias. Nowadays, th (about 10mM)e pharmacological targeting of is an ineludible therapeutic goal. The antidiabetic drug metformin was reported suppress in vitro vivo CSC survival different tumors and, particular, BC preclinical models. However, few...
The coordinated activity of estrogens and epidermal growth factor receptor (EGFR) family agonists represents the main determinant breast cancer cell proliferation. Stromal cell-derived factor-1 (SDF-1) enhances extracellular signal-regulated kinases 1 2 (ERK1/2) via transactivation EGFR 17β-estradiol (E2) induces SDF-1 production to exert autocrine proliferative effects. On this basis, we evaluated whether inhibition tyrosine kinase (TK) may control different mitogenic stimuli in tumors...
Summary Context First‐line therapy for thyrotropin‐secreting pituitary adenomas (TSHomas) is neurosurgery, while medical treatment rests mainly on somatostatin analogues. Clinically available sst 2 ‐preferring analogues, octreotide and lanreotide, induce normalization of hormone levels in approximately 90% patients tumour shrinkage 45%. Objective We evaluated 1, 2, 3 5 dopamine D2 receptor expression samples from three TSHomas, the relationships between expression, vitro antiproliferative...
Glioblastoma multiforme is a highly aggressive brain tumor whose prognosis very poor. Due to early invasion of parenchyma, its complete surgical removal nearly impossible, and even after combined treatment (association surgery chemo- radio-therapy) five-year survival only about 10%. Natural products are sources novel compounds endowed with therapeutic properties in many human diseases, including cancer. Here, we report that the water extract Ruta graveolens L., commonly known as rue, induces...
Somatostatin inhibits cell proliferation through the activation of five receptors (SSTR1–5) expressed in normal and cancer cells. We analyzed role individual SSTRs antiproliferative activity somatostatin C6 rat glioma dose-dependently inhibited proliferation, an effect mimicked, with different efficacy or potency, by BIM-23745, BIM-23120, BIM-23206 (agonists for SSTR1, -2, -5) octreotide. The SSTR3 was ineffective, although all are functionally active, as demonstrated inhibition cAMP...
Adiponectin (Acrp30) is an adipocyte-secreted hormone with pleiotropic metabolic effects, whose reduced levels were related to development and progression of several malignancies. We looked at the presence Acrp30 receptors in human glioblastomas (GBM), hypothesizing a role for also this untreatable cancer. Here we demonstrate that GBM express (AdipoR1 AdipoR2), which are often co-expressed samples (70% analyzed tumors). To investigate effects on growth, used cell lines U87-MG U251,...
Abstract: Stromal cell‐derived factor‐1 (SDF‐1) is a chemokine of the CXC subfamily that exerts its effects via CXCR4, G‐protein‐coupled receptor. CXCR4 often expressed by tumor cells, and activation causes cell proliferation. Using GH4C1 here we show SDF‐1 induced proliferation in dose‐dependent manner. Thus, evaluated intracellular signaling involved this effect. increased cytosolic [Ca 2+ ] activated Pyk2, ERK1/2, BK Ca channels. To correlate these effectors with proliferative activity...
Somatostatin receptors (SSTR1–5) mediate antiproliferative effects. In C6 rat glioma cells, somatostatin is cytostatic in vitro via phosphotyrosine phosphatase-dependent inhibition of ERK1/2 activity mediated by SSTR1, -2, and -5. Here we analyzed the effects SSTR activation on growth vivo intracellular mechanisms involved, comparing with selective agonists for -5 (BIM-23745, BIM-23120, BIM-23206) or receptor biselective compounds (SSTR1 BIM-23704; SSTR2 -5, BIM-23190). Nude mice...
Altered EGFR activity is a causal factor for human tumor development, including malignant pleural mesotheliomas. The aim of the present study was evaluation effects Gefitinib on EGF-induced mesothelioma cell proliferation and intracellular mechanisms involved. Cell proliferation, DNA synthesis apoptosis were measured by MTT, thymidine incorporation FACS analysis; EGFR, ERK1/2 Akt expression phosphorylation Western blot, whereas receptor sites analyzed binding studies. inhibited in two lines,...