A5052484166- Sperm and Testicular Function
- Reproductive biology and impacts on aquatic species
- Amphibian and Reptile Biology
- Lipid Membrane Structure and Behavior
- Reproductive Biology and Fertility
- Erythrocyte Function and Pathophysiology
- Animal Genetics and Reproduction
- Virus-based gene therapy research
- Animal Behavior and Reproduction
- Cancer Cells and Metastasis
- Veterinary Oncology Research
- Retinal Development and Disorders
- Physiological and biochemical adaptations
- Cancer Research and Treatments
- Photoreceptor and optogenetics research
- RNA Interference and Gene Delivery
- RNA Research and Splicing
- Neurobiology and Insect Physiology Research
- Connexins and lens biology
- Colorectal Cancer Treatments and Studies
- Cellular Mechanics and Interactions
- Neuropeptides and Animal Physiology
- Nuclear Structure and Function
- Chemokine receptors and signaling
- Plant and animal studies
Istituto Zooprofilattico Sperimentale del Piemonte Liguria e Valle d'Aosta
2011-2023
Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna "Bruno Ubertini"
2020
University of Naples Federico II
2004-2017
University of Salerno
2014
Istituto Nazionale di Fisica Nucleare, Sezione di Napoli
1993-1997
University of L'Aquila
1988-1992
Stazione Zoologica Anton Dohrn
1985
University of Florence
1984
University of Geneva
1980
Abstract In Xenopus laevis eggs, cisternae shells which surround cortical granules (CG) are part of a endoplasmic reticulum (ER) network. this paper the origin such ER has been studied in full‐grown, progesterone‐exposed oocytes. Furthermore, possible role activation process investigated by pricking maturing It shown that full‐grown ovarian oocytes CG absent and extensively randomly distributed throughout peripheral cytoplasm, where they appear to be continuous with annulate lamellae (AL)....
Cancer stem cells (CSCs) are considered the cell subpopulation responsible for breast cancer (BC) initiation, growth, and relapse. CSCs identified as self-renewing tumor-initiating cells, conferring resistance to chemo- radio-therapy several neoplasias. Nowadays, th (about 10mM)e pharmacological targeting of is an ineludible therapeutic goal. The antidiabetic drug metformin was reported suppress in vitro vivo CSC survival different tumors and, particular, BC preclinical models. However, few...
The egg of Discoglossus pictus has an animal dimple, a pit located at the hemisphere pole, which is only site successfully penetrated by sperm. Ultrastructural analysis unfertilized surface reveals unique features characterizing dimple. They are: 1) regularly spaced, finger-like microvilli supported bundles fibers appear to be microtubules and surrounded antennular glycocalyx, present orientation distribution suggest that they may play essential role in maintenance shape dimple: 2) granules...
The use of reference genes is commonly accepted as the most reliable approach to normalize qRT-PCR and reduce possible errors in quantification gene expression. suitable sheep have been identified for a restricted range tissues, but no specific data on whole blood are available. aim this study was identify set normalizing from ovine blood. We designed 11 PCR assays employed belonging various functional classes then determined their expression stability samples control disease-stressed sheep....
Abstract The distribution of actin, myosin, and tubulin has been investigated in coelomic oocytes, unfertilized fertilized eggs Discoglossus pictus utilizing: (1) immunofluorescence; (2) electron microscopy; (3) incubation with heavy meromyosin (HMM), (4) SDS‐polyacrylamide gel electrophoresis (PAGE). In the germinative area (GA) long, irregular microvilli containing microfilaments. rest oocyte, are shallow. During transit oocyte oviduct, a dimple forms by invagination GA. A palisade...
Abstract In the egg of Xenopus laevis a cortical network smooth endoplasmic reticulum (SER) surrounds and interconnects each granule (CG) (Campanella Andreuccetti, '77). This is possible intracellular site calcium storage to be called into action for CG exocytosis. our experiments, eggs, unfertilized or activated by pricking ionophore A 23187, have been fixed in osmium‐pyroantimonate localization. Our data show that deposits can detected only egs. The chelator edetate (EGTA) x‐ray microprobe...
Staphylococcal food poisoning (SFP) is one of the most important foodborne diseases. This work describes a SFP event linked to consumption alm cheese and involved three people belonging same family. Leftovers consumed cheese, samples from grocery store producing were collected tested for Coagulase positive staphylococci (CPS) enumeration presence staphylococcal enterotoxins (SEs). Isolates typed with MLST, spa typing, SEs methicillin resistance genes. An in vitro test evaluated production...
We have used ratiometric confocal microscopy and three fluorescence techniques to study the distribution activity of mitochondria in frog oocytes during early stages oogenesis. Mitochondria oogenesis were characterised by a high ratio ‘mitochondrial cloud’ peri-nuclear region low freely dispersed within cytoplasm. tested whether visualised represented mitochondrial membrane potential perturbing potential. Carbonyl cyanide p -(trifluoromethyl)phenylhydrazone (FCCP) caused immediate...
The ultrastructure of sperm changes and penetration in the egg was studied anuran Discoglossus pictus, whose have an acrosome cap with a typical tip, apical rod. first stage rod reaction (AR) consists vesiculation between plasma membrane outer membrane. two components are released sequence. innermost component (component B) is dispersed first. next change dispersal outermost content A). At 30 sec postinsemination, when loss B observed, holes seen jelly coat (J1), surrounding penetrating...
Abstract Background Mammary tumours frequently develop in female domestic cats being highly malignant a large percentage of cases. Chemokines regulate many physiological and pathological processes including organogenesis, chemotaxis inflammatory cells, as well tumour progression metastasization. In particular, the chemokine/receptor pair SDF-1/CXCR4 has been involved regulation metastatic potential neoplastic breast cancer. The aim this study was immunohistochemical defininition expression...