A5041271863- CRISPR and Genetic Engineering
- Occupational and environmental lung diseases
- Head and Neck Cancer Studies
- Gastric Cancer Management and Outcomes
- Medical Imaging and Pathology Studies
- Gastrointestinal Tumor Research and Treatment
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Pleural and Pulmonary Diseases
- Cancer-related gene regulation
Université de Bordeaux
2025
Institut Curie
2021
Hôpital Lyon Sud
2017
Hospices Civils de Lyon
2017
Genome editing by CRISPR-Cas9 is promising for genetic disease and cancer gene therapy. However, safety concerns are still present, particularly the ON-target genotoxicity protocols using nucleases. Quality control of edited cells before after graft mandatory, especially to assay megabase-scale genomic rearrangements induced at targeted locus. These unintended events fortunately rare but potentially deleterious. Classical PCR-based bulk approaches do not detect them or underestimate their...
<title>Abstract</title> Genome editing by CRISPR-Cas9 is promising for genetic disease and cancer gene therapy. However, safety concerns are still present, particularly the ON-target genotoxicity protocols using nucleases. Quality control of edited cells before after graft mandatory, especially to assay megabase-scale genomic rearrangements induced at targeted locus. These unintended events fortunately rare but potentially deleterious. Classical PCR-based bulk approaches do not detect them...
Oral cavity is the most prevalent site of head and neck squamous cell carcinomas (HNSCCs). Most often diagnosed at a locally advanced stage, treatment multimodal with surgery as cornerstone. The aim this study was to explore molecular landscape homogenous cohort oral (OCSCCs), assess prognostic value tumor mutational burden (TMB), along classical clinical parameters.One hundred fifty-one consecutive patients OCSCC treated upfront Institut Curie were analyzed. Sequencing DNA from frozen...