A5082523676- Antioxidant Activity and Oxidative Stress
- Pharmaceutical and Antibiotic Environmental Impacts
- Phytochemicals and Antioxidant Activities
- Advanced biosensing and bioanalysis techniques
- Reproductive tract infections research
- Free Radicals and Antioxidants
- Fecal contamination and water quality
- Biosensors and Analytical Detection
- Vitamin C and Antioxidants Research
- Water Treatment and Disinfection
- Food Chemistry and Fat Analysis
- Morinda citrifolia extract uses
- Arsenic contamination and mitigation
- Wastewater Treatment and Nitrogen Removal
- Antibiotic Resistance in Bacteria
- Microbial Community Ecology and Physiology
- Gout, Hyperuricemia, Uric Acid
- Atherosclerosis and Cardiovascular Diseases
- Analytical chemistry methods development
- Cholesterol and Lipid Metabolism
- Inflammasome and immune disorders
- Behavioral and Psychological Studies
- Salmonella and Campylobacter epidemiology
- Attention Deficit Hyperactivity Disorder
- Peroxisome Proliferator-Activated Receptors
National Defense Medical College
2000-2002
Ibaraki Christian University
2001
National Institute of Infectious Diseases
1996-2001
Ochanomizu University
1996-1997
National Institute of Nutrition
1997
Norsk Hydro (Germany)
1957
Marine animals produce astaxanthin which is a carotenoid and antioxidant. In this study we determined the in vitro ex vivo effects of on LDL oxidation. The oxidation was measured 1 ml reaction system consisting increasing concentrations (12.5, 25.0, 50.0 μg/ml), 400 μM V-70 (2, 2'-azobis (4-methoxy-2, 4-dimethylvaleronitrile)), (70 μg/ml protein). Astaxanthin dose, dependently significantly prolonged lag time (31.5, 45.4, 65.0 min) compared with control (19.9 min). For 24 volunteers (mean...
Flavonoids, a group of polyphenolic compounds, exist naturally and serve as antioxidants in vegetables, fruits, so on. The inhibition low density lipoprotein (LDL) oxidation may be an effective way to prevent or delay the progression atherosclerosis. In present study, we analyzed radical scavenging capacity 10 flavonoids (catechin, epicatechin [EC], epigallocatechin [EGC], gallate [ECg], [EGCg], myricetin, quercetiji, apigenin, kaempferol, luteolin) toward 1, 1-Biphenyl-2-picryl-hydrazyl...
An important event in the pathogenesis of atherosclerosis is believed to be oxidative modification low-density lipoprotein (LDL) initiated by a free radical-driven lipid peroxidation process. Vitamin E acts as lipophilic chain-breaking antioxidant, while water-soluble antioxidants such vitamin C or uric acid suppress oxidation LDL aqueous radicals. In this study, we established new method measuring lag time inhibited using azo radical initiator V-70: 2-2'-azobis(4-methoxy-2,...
The effects of cacao liquor polyphenols (CLP) on the susceptibility low-density lipoprotein (LDL) to oxidation in hypercholesterolemic rabbits were examined. Six Japanese white which had been fed a high cholesterol diet (HCD) for 3 weeks HCD containing 1% CLP following 10 days. LDL induced by 2-2'-azobis (4-methoxy-2, 4-dimethylvaleronitrile) (V-70) was evaluated measuring production conjugated dienes and thiobarbituric acid reactive substances (TBARS). lag time significantly prolonged from...
Cacao is rich in polyphenols such as (-)-epicatechin, and a colored compo-nent of cacao (cacao-red) polyphenol, which an antioxidant. These properties stimu-lated investigation the effects liquor (CLP) on low-density lipoprotein (LDL) oxidation. The 2, 2'-azobis(4-methoxy-2, 4-dimethylvaleronitrile) (AMVN-CH3O)-induced oxidizability LDL was assessed by monitoring absorbance at 234 nm. In vitro, 0.1-0.5mg/dL CLP prolonged oxidation lag time dose-dependent manner. Compared with controls, it...
Abstract Cholesteryl ester transfer protein (CETP) is an important determinant of lipoprotein function, especially high density (HDL) metabolism, and contributes to the regulation plasma HDL levels. Since saturated polyunsaturated fatty acids (FA) appear influence CETP activity differently, we decided investigate effects FA on expression mRNA in HepG2 cells using RNA blot hybridization analysis. Long‐chain (>18 carbons) at a 0.5 mM concentration were added medium incubated with for 48 h...
Recently, wastewater treatment plants have been identified as potential reservoirs of antibiotic-resistant bacteria. Hence, it is important to monitor bacteria in plants. Currently available methods are laborious, time-consuming, and costly. In this study, a novel simple phenotypic antibiotic-susceptibility testing method for Escherichia coli was developed. A growth curve E. the presence an antibiotic established by monitoring fluorescence intensity fluorogenic substrate specific using...
Abstract Monitoring of Escherichia coli concentrations in river water (RW) is essential to identify fecal pollution the river. The objective this study was assess suitability a novel, simple and high throughput method developed our laboratory enumerate E. RW samples. based on use synthetic substrate specific for β-d-glucuronidase (GUS) produced by coli. GUS activities were monitored at eight selected sites rivers running through Sapporo, Japan. Because fluorescence intensities samples...
Abstract Arsenic (As) contamination in groundwater presents a major health and environmental concern. As is found two oxidation states most chemical tests for inorganic arsenic are focused on As(III), few have been developed As(V). We the simple analytical method determining As(V) concentrations using CeO2NPs fluorescein (FAM)-labeled DNA. Prior to sample measurements, we investigated key operational parameters that affect sensing performance. The optimal final concentration, FAM-labeled DNA...
ABSTRACT To investigate the physicochemical conditions necessary to stably remove antibiotic-resistant bacteria (ARB) via contact with activated sludge (AS), adhesion of ciprofloxacin (CIP)-resistant and -susceptible Escherichia coli AS was simulated by tests in laboratory. The CIP-resistant E. susceptible were removed a 3 log smaller concentration 5 h test at maximum. Considering hydraulic retention time reaction tank (∼5 h) step-feeding operation, we considered removal rate current be...
The identification of the bacteria contributing to water purification processes in these systems is crucial for development and optimization wastewater treatment by engineers. We developed a simple assay colorimetric quantification bacterial 16S rRNA functional extracted from samples without reverse transcription RNA or quantitative polymerase chain reaction (qPCR). heat test sample significantly reduced time (<3 min) improved sensitivity. Following optimization, calibration curve...
Application of 10%, 20%, or 48% hydrobromic acid to 5-bromo-6-hydroxyauinoline (I) 7-hydroxy-8-bromoquinoline (II) resulted in recovery the starting material either case. The same reaction carried presence phenol was found cause quantitative debromination case and a debrominated compound obtained high yield from 20% acid. true 5-bromo-6-aminoquinoline with phenol. Debromination did not occur 6-hydroxy-7-bromoquinoline (V) acid, even
RNA is a crucial diagnostic biomarker for several human diseases. Hence, simple, rapid, sensitive, and cost-effective assay detecting pathogen-derived RNAs (e.g., viruses) in water necessary transcriptomic analysis. Here, we present highly sensitive to detect unamplified based on the light scattering properties of oligonucleotide-functionalized gold nanoparticle probes (Au-nanoprobes). In this assay, Au-nanoprobes recognize hybridize target RNA. Subsequently, they scatter when sample...