A5041443588- Genetic Neurodegenerative Diseases
- Mitochondrial Function and Pathology
- Gene expression and cancer classification
- Bioinformatics and Genomic Networks
- Single-cell and spatial transcriptomics
- Cell Image Analysis Techniques
- RNA Research and Splicing
- Lysosomal Storage Disorders Research
- Neurological disorders and treatments
- Functional Brain Connectivity Studies
- Medical Image Segmentation Techniques
- Muscle Physiology and Disorders
- Advanced Neuroimaging Techniques and Applications
- Neurotransmitter Receptor Influence on Behavior
- Cellular transport and secretion
- Genomics and Chromatin Dynamics
- Occupational and environmental lung diseases
- Hereditary Neurological Disorders
- Health, Environment, Cognitive Aging
- Trypanosoma species research and implications
- Venomous Animal Envenomation and Studies
- Memory and Neural Mechanisms
- Connective tissue disorders research
- Pleural and Pulmonary Diseases
- DNA Repair Mechanisms
RIKEN Center for Advanced Photonics
2018
Jissen Women's University
2018
RIKEN Advanced Science Institute
2011-2014
RIKEN Center for Integrative Medical Sciences
2005-2012
National Center For Child Health and Development
1998-2003
Tokyo Metropolitan Institute of Medical Science
1997
University of Toronto
1996
Hospital for Sick Children
1993-1994
The University of Tokyo
1984
We and others have previously shown that a 67-kD cell surface elastin/laminin-binding protein (EBP) is responsible for adhesion to elastin laminin mediating the process of fiber assembly, but nature this was unknown. In report we provide evidence catalytically inactive form beta-galactosidase produced by alternative splicing demonstrates immunological functional similarity sequence homology EBP, suggesting two might be same. Antibody prepared synthetic peptide, N-Ac-GSPSAQDEASPL,...
Background Mesothelioma is a highly malignant tumor that primarily caused by occupational or environmental exposure to asbestos fibers. Despite worldwide restrictions on usage, further cases are expected as diagnosis typically 20–40 years after exposure. Once diagnosed there very poor prognosis with median survival rate of 9 months. Considering this the development early pre clinical diagnostic markers may help improve outcomes. Methodology Microarray expression arrays mesothelium and other...
Dentatorubral pallidoluysian atrophy (DRPLA) is an autosomal dominant neurodegenerative disorder. It associated with abnormal CAG repeat expansion resulting in formation of a protein elongated polyglutamine stretch. However, neither the physiological roles DRPLA gene product nor molecular mechanisms its pathogenesis have yet been elucidated. Here we report that cleaved at site near N terminus during apoptosis induced by VP-16, staurosporine, or glucocorticoid. Moreover, vitro translated...
Dentatorubral-pallidoluysian atrophy (DRPLA) is an autosomal dominant neuro degrees enerative disorder associated with CAG/glutamine repeat expansion. While the DRPLA gene ubiquitously expressed, neuron death occurs in specific anatomical areas of brain. This predicts that protein interacts other proteins and these interactions may play a role pathogenesis. Here, we describe binds to product. One clones isolated yeast two-hybrid system was identified as human homolog insulin receptor...
Chinese hamster ovary cell clones permanently transfected with the cDNA for human lysosomal beta-galactosidase secrete enzyme precursor into medium, from which it is purified to apparent homogeneity in a single step by affinity chromatography. The fully active, displays same pH optimum and Km values as mature placental enzyme, has an intact C-terminus. when chromatographed on Sephacryl S-200 molecular-sieve column elutes 105,500 Da monomer, whereas SDS/PAGE gels polypeptide migrates 88 kDa...
Increased information on the encoded mammalian genome is expected to facilitate an integrated understanding of complex anatomical structure and function based knowledge gene products. Determination expression-anatomy associations crucial for this understanding. To elicit association in three-dimensional (3D) space, we introduce a novel technique comprehensive mapping endogenous expression into web-accessible standard space: Transcriptome Tomography. The conjugation sequential tissue-block...
Processing of human beta-galactosidase (beta-GAL) was studied in permanently transfected Chinese hamster ovary (CHO) cells and compared with that normal from subjects GM1-gangliosidosis, galactosialidosis I-cell disease. Biosynthesis beta-GAL CHO results the synthesis an 88 kDa glycosylated phosphorylated monomer precursor which is enzymically active secreted into medium. Post-translational processing begins at C-terminal end protein gives rise to structurally related 67 64 mature forms....
Dentatorubral-pallidoluysian atrophy (DRPLA) is a dominant-inherited neurodegenerative disease characterized by selective cell loss in particular neuronal pathways. This caused expansion of CAG repeats the coding region DRPLA gene, and extended polyglutamine tract (polyQ) confers toxic activity. It valuable to characterize gene products for elucidation mechanism underlying neuron death at specific anatomical areas brain. Here, we define protein as phosphoprotein, c-Jun NH2-terminal kinase...
Lysosomal β‐galactosidase precursor is processed to a mature form and associated with protective protein in lysosomes. In this study we used two cysteine protease proinhibitors, E64‐d for cathepsins B, S, H, L, CA074Me cathepsin B. They are converted intracellularly active forms, E‐64c CA074, respectively. Both compounds inhibited maturation of the exogenous precursor, but did not inhibit further degradation an inactive 50‐kDa product. We concluded that B participated exclusively...
Recent analyses have suggested that many genes possess multiple transcription start sites (TSSs) are differentially utilized in different tissues and cell lines. We identified a huge number of TSSs mapped onto the mouse genome using cap analysis gene expression (CAGE) method. The standard hierarchical clustering algorithm, which gives us easily understandable graphical tree images, has difficulties processing such amounts TSS data better method to calculate display results is needed. use...
Understanding anatomical structures and biological functions based on gene expression is critical in a systemic approach to address the complexity of mammalian brain, where >25 000 genes are expressed precise manner. Co-expressed thought regulate cell type- or region-specific brain functions. Thus, well-designed data acquisition visualization systems for profiling combinatorial relation crucial. To this purpose, using our techniques microtomy-based measurements WebGL-based programs, we...
Using a recently invented technique for gene expression mapping in the whole-anatomy context, termed transcriptome tomography, we have generated dataset of 36,000 maps overall adult-mouse brain. Here, using an informatics approach, identified broad co-expression network that follows inverse power law and is rich functional interaction gene-ontology terms. Our framework integrated analysis graphs networks revealed groups combinatorially expressed genes, which regulate cell differentiation...
Motor functions of the biological system has been forged through 4 billion years evolution. From a neurorobotics view, it is important not only to know how well works, but also fails. To quantitatively describe early onset symptoms neurodegenerative disease, we analyzed phenotypes genetically engineered Huntington disease (HD) model mice, which reveal progressive impaired motor functions. We devised simple yet sensitive paradigm called crystalized motion profile (CMP), by successfully...
Event Abstract Back to Integrated Analysis of Anatomical Gene Expression Maps and Co-Expression Networks Using a Database, ViBrism Yuko Okamura-Oho1*, Kazuro Shimokawa2, Satoko Takemoto3, Gang Song4, James Gee4 Hideo Yokota3 1 BReNt-Brain Research Network Advanced Science Institute, RIKEN, Japan 2 Department Diagnostics Therapeutics, National Center for Global Health Medicine, 3 Bio-research Infrastructure Construction Team, RIKEN , 4 Penn Image Computing Laboratory, University Pennsylvania,...