A5005621524- Mitochondrial Function and Pathology
- Metabolism and Genetic Disorders
- Cellular transport and secretion
- Amino Acid Enzymes and Metabolism
- Genetic and Kidney Cyst Diseases
- Advanced Fluorescence Microscopy Techniques
- Forensic and Genetic Research
- Cell Image Analysis Techniques
- Protist diversity and phylogeny
- Microtubule and mitosis dynamics
- RNA modifications and cancer
- Molecular Biology Techniques and Applications
- Endoplasmic Reticulum Stress and Disease
- Autophagy in Disease and Therapy
- Video Surveillance and Tracking Methods
- Erythrocyte Function and Pathophysiology
- Cancer, Hypoxia, and Metabolism
- Target Tracking and Data Fusion in Sensor Networks
- Lipid Membrane Structure and Behavior
- RNA and protein synthesis mechanisms
- Spectroscopy Techniques in Biomedical and Chemical Research
- ATP Synthase and ATPases Research
- Genomics and Phylogenetic Studies
- Epigenetics and DNA Methylation
- Porphyrin Metabolism and Disorders
Yale University
2010-2025
Zhejiang University
2024
Soochow University
2022-2024
First Affiliated Hospital of Soochow University
2022-2024
National Taiwan University
2024
Broad Institute
2017-2023
Systems Biology Institute
2022
Howard Hughes Medical Institute
2009-2021
Center for Systems Biology
2017-2021
Harvard University
2017-2021
The first community competition designed to objectively compare the performance of particle tracking algorithms provides valuable practical information for both users and developers. Particle is key importance quantitative analysis intracellular dynamic processes from time-lapse microscopy image data. Because manually detecting following large numbers individual particles not feasible, automated computational methods have been developed these tasks by many groups. Aiming perform an objective...
Dynamin, which is encoded by three genes in mammals, a GTPase implicated endocytic membrane fission. Dynamin 1 and 3 are predominantly expressed brain, while dynamin 2 ubiquitously expressed. With the goal of assessing impact lack on cell physiology, we previously generated characterized double KO (DKO) fibroblasts. These DKO cells were unexpectedly viable spite severe impairment clathrin-mediated endocytosis. As low-level expression gene these could not be excluded, have now engineered 1,...
Epstein-Barr virus (EBV) causes Burkitt, Hodgkin, and post-transplant B cell lymphomas. How EBV remodels metabolic pathways to support rapid outgrowth remains largely unknown. To gain insights, primary human cells were profiled by tandem-mass-tag-based proteomics at rest nine time points after infection; >8,000 host 29 viral proteins quantified, revealing mitochondrial remodeling induction of one-carbon (1C) metabolism. EBV-encoded EBNA2 its target MYC required for upregulation the central...
Mitochondria, which are excluded from the secretory pathway, depend on lipid transport proteins for their supply ER, where most lipids synthesized. In yeast, outer mitochondrial membrane GTPase Gem1 is an accessory factor of ERMES, ER–mitochondria tethering complex that contains domains and functions, partially redundantly with Vps13, in transfer between two organelles. metazoa, VPS13, but not present, orthologue Miro was linked to dynamics transport. Here we show Miro, including its...
Abstract Glucose is vital for life, serving as both a source of energy and carbon building block growth. When glucose limiting, alternative nutrients must be harnessed. To identify mechanisms by which cells can tolerate complete loss glucose, we performed nutrient-sensitized genome-wide genetic screens PRISM growth assay across 482 cancer cell lines. We report that catabolism uridine from the medium enables in absence glucose. While previous studies have shown salvaged to support pyrimidine...
The role of endocytosis in the control EGF receptor (EGFR) activation and cell signaling was explored by using mouse fibroblasts which dynamin conditionally depleted. Dynamin is a GTPase shown to play an important clathrin mediated EGFR other surface receptors. In this report, we demonstrate that binding activity display high low affinity EGFRs on are not affected depletion. By contrast, depletion leads strong inhibition endocytosis, robust enhancement autophosphorylation ubiquitination,...
Itaconate brings metalloenzyme to a halt Controlled radicals enable unusual enzymatic transformations, but radical generation and management require dedicated systems. Ruetz et al. investigated how the immunometabolite itaconate might undermine these intricate systems inhibit propionate metabolism, crucial metabolic pathway in pathogenic Mycobacterium tuberculosis (Mtb) (see Perspective by Boal). They found that coenzyme A (CoA) derivative of can irreversibly enzyme methylmalonyl-CoA mutase...
The majority of mitochondrial proteins are encoded in the nuclear genome, translated cytoplasm, and directed to mitochondria by an N-terminal presequence that is cleaved upon import. Recently, N-proteome catalogs have been generated for from yeast human U937 cells. Here, we applied subtiligase method determine N-termini 327 isolated mouse liver kidney. Comparative analysis between mouse, human, shows whereas presequences poorly conserved at sequence level, other properties extremely...
Abstract The SLC25 carrier family consists of 53 transporters that shuttle nutrients and co-factors across mitochondrial membranes. is highly redundant their transport activities coupled to metabolic state. Here, we use a pooled, dual CRISPR screening strategy knocks out pairs in four states — glucose, galactose, OXPHOS inhibition, absence pyruvate designed unmask the inter-dependence these genes. In total, screen 63 genes states, corresponding 2016 single pair-wise genetic perturbations. We...
Abstract Autosomal dominant polycystic kidney disease (ADPKD) is the most prevalent potentially lethal monogenic disorder. Mutations in PKD1 gene, which encodes polycystin-1 (PC1), account for approximately 78% of cases. PC1 a large 462-kDa protein that undergoes cleavage its N and C-terminal domains. produces fragments translocate to mitochondria. We show transgenic expression corresponding final 200 amino acid (aa) residues two Pkd1 -KO orthologous murine models ADPKD suppresses cystic...
Pulmonary infection is common yet serious complication in patients with severe traumatic brain injury (STBI). We aimed to evaluate the predicators of pulmonary STBI undergoing tracheostomy, provide evidence for clinical nursing care patients.This study was a retrospective cohort design. tracheostomy treatment from January 1, 2019 August 31, 2021 our hospital were included. The characteristics and no analyzed.A total 216 included, incidence 26.85%. Diabetes (r = 0.782), hypoproteinemia...
Emerging evidence has shown that epigenetic regulation plays a fundamental role in cancer metastasis, the major cause of cancer-related deaths. Here, we conducted an vivo screen for vulnerabilities brain metastasis and identified N -acetyltransferase 10 (NAT10) as driver metastasis. Knockdown NAT10 restrains cell proliferation migration vitro tumor growth vivo. The poorly characterized RNA helicase domain is critical vitro, while both NAT domains are essential primary Mechanically, promotes...
Clathrin-mediated endocytosis is a fundamental cellular process conserved from yeast to mammals and an important endocytic route for the internalization of many specific cargos, including activated growth factor receptors. Here we examined changes in tyrosine phosphorylation, representative output receptor signaling, cells which clathrin-coated pits are frozen at deeply invaginated state, that is, lack dynamin (fibroblasts 1, 2 double conditional knockout mice). The major change observed...
In order to quantitatively analyze biological images and study underlying mechanisms of the cellular subcellular processes, it is often required track a large number particles involved in these processes. Manual tracking can be performed by biologists, but workload very heavy. this paper, we present an automatic particle method for analyzing essential process, namely clathrin mediated endocytosis. The framework extension classical multiple hypothesis (MHT), designed manage trajectories,...