A5100333160- DNA Repair Mechanisms
- Metabolomics and Mass Spectrometry Studies
- Alzheimer's disease research and treatments
- Bacterial Genetics and Biotechnology
- Intracerebral and Subarachnoid Hemorrhage Research
- Microbial Metabolic Engineering and Bioproduction
- Tryptophan and brain disorders
- Amino Acid Enzymes and Metabolism
- Fetal and Pediatric Neurological Disorders
- Cerebrospinal fluid and hydrocephalus
- Microbial Metabolites in Food Biotechnology
- Enzyme Production and Characterization
- MicroRNA in disease regulation
- Gut microbiota and health
- Phytase and its Applications
- DNA and Nucleic Acid Chemistry
- Aquaculture Nutrition and Growth
- Apelin-related biomedical research
- Gestational Diabetes Research and Management
- Nerve injury and regeneration
- Advanced Proteomics Techniques and Applications
- Parkinson's Disease Mechanisms and Treatments
- Effects of Radiation Exposure
- Birth, Development, and Health
- Paraoxonase enzyme and polymorphisms
Shanghai Advanced Research Institute
2023-2025
Chinese Academy of Sciences
2022-2025
W. M. Keck Foundation
2018-2025
Nanjing University of Chinese Medicine
2025
Nanjing Drum Tower Hospital
2025
Shanxi Agricultural University
2024
Yale University
2018-2023
Shanghai Institute of Applied Physics
2022
University of Chinese Academy of Sciences
2022
Xiamen University
2021
Colibactin is a complex secondary metabolite produced by some genotoxic gut Escherichia coli strains. The presence of colibactin-producing bacteria correlates with the frequency and severity colorectal cancer in humans. However, because colibactin has not been isolated or structurally characterized, studying physiological effects human difficult. We used combination genetics, isotope labeling, tandem mass spectrometry, chemical synthesis to deduce structure colibactin. Our structural...
The guanosine triphosphatase (GTPase) Rab32 coordinates a cell-intrinsic host defense mechanism that restricts the replication of intravacuolar pathogens such as Salmonella Here, we show this requires aconitate decarboxylase 1 (IRG1), which synthesizes itaconate, metabolite with antimicrobial activity. We find interacts IRG1 on infection and facilitates delivery itaconate to Salmonella-containing vacuole. Mice defective in rescued virulence defect S. enterica serovar Typhimurium mutant...
Idiopathic normal pressure hydrocephalus (iNPH) is a reversible neurologic disorder that remains poorly understood. Accurate differential diagnosis of iNPH and Alzheimer disease (AD) complicated by overlapping clinical manifestations. Beyond neuroimaging, there are currently no biomarkers available for leading to frequent misdiagnosis, proteomic studies into have been limited low sample sizes inadequate analytical depth. In this study, we report the results large-scale analysis CSF from...
Abstract Autosomal dominant polycystic kidney disease (ADPKD) is the most prevalent potentially lethal monogenic disorder. Mutations in PKD1 gene, which encodes polycystin-1 (PC1), account for approximately 78% of cases. PC1 a large 462-kDa protein that undergoes cleavage its N and C-terminal domains. produces fragments translocate to mitochondria. We show transgenic expression corresponding final 200 amino acid (aa) residues two Pkd1 -KO orthologous murine models ADPKD suppresses cystic...
Colibactins are genotoxic secondary metabolites whose biosynthesis is encoded in the clb gene cluster harbored by certain strains of gut commensal Escherichia coli. Using synthetic colibactin analogues, we previously provided evidence that colibactins alkylate DNA addition a nucleotide to an electrophilic cyclopropane intermediate. However, natural colibactin-nucleobase adducts have not been identified, best our knowledge. Here present first identification such adducts, derived from...
Objective Systemic sclerosis–associated interstitial lung disease (SSc‐ILD) is characterized by variable clinical outcomes, activation of innate immune pattern‐recognition receptors (PRRs), and accumulation α‐smooth muscle actin (α‐SMA)–expressing myofibroblasts. The aim this study was to identify an association between these entities mitochondrial DNA (mtDNA), endogenous ligand for the intracellular DNA–sensing PRRs Toll‐like receptor 9 (TLR‐9) cyclic GMP‐AMP synthase/stimulator interferon...
Abstract Alzheimer’s disease (AD) is a complex and heterogeneous neurodegenerative disorder with contributions from multiple pathophysiological pathways. One of the long-recognized important features AD disrupted cerebral glucose metabolism, but underlying molecular basis remains unclear. In this study, unbiased mass spectrometry was used to survey CSF large clinical cohort, comparing patients who are either cognitively unimpaired (CU; n = 68), suffering mild-cognitive impairment or dementia...
Heat-shock protein 90 (HSP90) is a highly active molecular chaperone that plays crucial role in cellular function. It facilitates the folding, assembly and stability of various oncogenic proteins, particularly kinases transcription factors involved regulating tumor growth maintenance signaling pathways. Consequently, HSP90 inhibitors are being explored as drugs for cancer therapy. Crystallographic fragment screening novel method has been developed recent years fragment-based drug discovery...
Abstract Background Post-traumatic stress disorder (PTSD) is a common and disabling psychiatric disorder. PTSD involves multiple brain regions often comorbid with other disorders, such as major depressive (MDD). Recent genome-wide association studies (GWASs) have identified many risk loci transcriptomics of postmortem found differentially expressed genes associated cases. In this study, we integrated measures across modalities to identify convergent molecular effects in the brain. Methods We...
Opioid use disorder (OUD) is a public health crisis currently being exacerbated by increased rates of and overdose synthetic opioids, primarily fentanyl. Therefore, the identification novel biomarkers treatment strategies to reduce problematic fentanyl relapse taking critical. In recent years, there has been growing body work demonstrating that gut microbiome can serve as potent modulator behavioral transcriptional responses both stimulants opioids. Here, we advance this define how...
The aim of this study was to screen molecular biomarkers for biodosimetry from DNA repair-related gene expression profiles.Mice were subjected whole-body exposure with 60Co gamma rays a dose range 0-8 Gy at rate 0.80 Gy/min. RNA extracted the peripheral blood irradiated mice 4, 8, 12, 24 and 48hrs post-irradiation. mRNA transcriptional changes 11 genes related damage repair detected using real-time quantitative polymerase chain reaction (RT-PCR).Of examined, CDKN1A (cyclin-dependent kinase...
(Hyper)thermophilic enzymes derived from (hyper)thermophilic microorganisms have significant implications for both basic research and industrial applications. Investigating the thermal stability catalytic mechanisms of thermophilic a structural perspective is essential rational modification these enzymes. Currently, X-ray crystallography remains one key techniques determining structures biomolecules; however, protein crystallization multiparameter-regulated often unpredictable process. Here,...
In recent years, the use of endocrine-disrupting chemicals (EDCs) has become increasingly common, leading to severe environmental pollution and harm aquatic organisms. 17α-Methyltestosterone (MT) is a synthetic androgen that can cause immunotoxicity in aquaculture, affecting fish health. To address this issue, study aimed investigate effect Vitamin C (VC) on MT-induced determine optimal VC supplementation.
Abstract Colibactin is a gut microbiome metabolite of unknown structure that has been implicated in colorectal cancer formation. Several studies now suggest the tumorgenicity colibactin derives from interstrand cross-linking host DNA. Here we use combination genetics, isotope labeling, tandem MS, and chemical synthesis to deduce colibactin. Our structural assignment accounts for all known biosynthetic data suggests roles final unaccounted enzymes gene cluster. DNA cross-link degradation...