A5101667343- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Hepatitis B Virus Studies
- Immune Response and Inflammation
- Cytomegalovirus and herpesvirus research
- Viral Infections and Immunology Research
- Hepatitis C virus research
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Virus-based gene therapy research
- Advanced Glycation End Products research
- Liver Disease Diagnosis and Treatment
- Cancer Immunotherapy and Biomarkers
- Regulation of Appetite and Obesity
- Systemic Lupus Erythematosus Research
- interferon and immune responses
- Advanced Biosensing Techniques and Applications
- Organ Transplantation Techniques and Outcomes
- Rheumatoid Arthritis Research and Therapies
- Xenotransplantation and immune response
- Monoclonal and Polyclonal Antibodies Research
- Inflammasome and immune disorders
- Mosquito-borne diseases and control
- Signaling Pathways in Disease
Children's Hospital of Fudan University
2024
Zhejiang University
2010-2021
Shenzhen University Health Science Center
2020
Changhai Hospital
2009-2019
Second Military Medical University
2009-2019
Government of Jilin Province
2019
Cleveland Clinic Lerner College of Medicine
2008-2012
Cleveland Clinic
2008-2012
Institute of Immunology
2009
302 Military Hospital of China
2005
High mobility group box-1 (HMGB1) protein was originally characterized as a nuclear DNA-binding protein, and described to have an extracellular role when involved in cellular activation proinflammatory responses. In the present study, we found that activity of recombinant HMGB1 proteins is determined by containing endotoxin level, contains few endotoxins fails stimulate macrophages secrete cytokines. acts ligand receptor for advanced glycation end products (RAGE) works synergy with LPS...
Rheumatoid arthritis (RA) is a complex and not fully understood autoimmune disease associated with multijoint damage. The main effector cells, the synovial fibroblasts, are apoptosis resistant hyperplastic which indicate that autophagy level high in tissue. Real-time PCR, immunocytochemistry, western blotting were used this paper to study status of tissues obtained from RA OA patients at time joint replacement surgery. We further evaluated correlation between levels activity-associated serum...
It is generally believed that some inflammatory antigens can recognize Toll-like receptors on synovial fibroblasts (SFs) and then activate downstream signals, leading to the formation of RASFs inducing rheumatoid arthritis (RA). The objective current work was study hypothesis outer PAMP (LPS) binds inner DAMP (HMGB1) becomes a complex recognizes TLRs/RAGE SFs, thus initiating signaling cascade leads secretion cytokines chemokines, production tissue-destructive enzymes, RASFs, finally...
Abstract IL -10 is widely accepted as a survival, proliferation, and differentiation factor for B cells. However, IL-10 deficiency accelerates disease progression the result of autoantibody production in many autoimmune models. It was demonstrated that T follicular helper cells (TFH cells) play key role helping are secreting Abs. In this study, we regulatory IL-10R signaling on development cell help function TFH vitro vivo. IL-1R subunit β-deficient (Il10rb−/−) Th were able to differentiate...
Synovial fibroblasts (SF) play a central role in the inflammatory and destructive process rheumatoid arthritis (RA). High-mobility group box chromosomal protein 1 (HMGB1) or lipopolysaccharide (LPS) alone failed to induce significant changes proliferation of cultured SF from RA patients, but premixed HMGB1 with LPS (HMGB1-LPS) significantly facilitated proliferation. IL-6, MMP-3, MMP-13 production greatly enhanced LPS-induced expression at both mRNA levels. HMGB1-LPS synergistically...
The P. falciparum chimeric protein 2.9 (PfCP-2.9) consisting of the sequences MSP1-19 and AMA-1 (III) is a malaria vaccine candidate that was found to induce inhibitory antibodies in rabbits monkeys. This phase I randomized, single-blind, placebo-controlled, dose-escalation study evaluate safety immunogenicity PfCP-2.9 formulated with novel adjuvant Montanide ISA720. Fifty-two subjects were randomly assigned 4 dose groups 10 participants, each receiving test 20, 50, 100, or 200 microg...
Background:Circulating microRNA (miRNA) are promising biomarkers for diagnosing and prognosticating numerous diseases. Reports have demonstrated controversial or even contradictory conclusions in studies on circulating microRNA. This study aimed to evaluate the potential bias of using different reference genes analyzing microRNAs same malignant digestive Material andMethods:We measured plasma concentrations U6-snRNA, let-7a, miRNA-21, miRNA-106a, miRNA-155, miRNA-219, miRNA-221, miRNA-16...
Abstract Excessive activation of TLR may induce endotoxin shock and inflammatory diseases, so the negative regulation TLR-triggered response attracts much attention. Nonpathogenic immune complex (IC) Ig (IC/Ig) have been shown to play important roles in responses be therapeutic some kinds autoimmune diseases. However, role IC/Ig underlying mechanisms remain fully understood. In this study we demonstrate that can significantly inhibit LPS-induced secretion TNF-α IL-6 from macrophages by...
In this work, we aimed to investigate the frequency, possible categories and clinical significance of circulating CD4 + ICOS FoxP3 T cells in patients with systemic lupus erythematosus (SLE). The frequency was analysed by flow-cytometric analysis 32 SLE patients, 10 rheumatoid arthritis healthy controls. Production IL-10 mTGF-β different T-cell populations determined intracellular cytokine staining. Plasma levels TGF-β were enzyme-linked immunosorbent assay (ELISA). significantly increased...
The aim of this study was to screen molecular biomarkers for biodosimetry from DNA repair-related gene expression profiles.Mice were subjected whole-body exposure with 60Co gamma rays a dose range 0-8 Gy at rate 0.80 Gy/min. RNA extracted the peripheral blood irradiated mice 4, 8, 12, 24 and 48hrs post-irradiation. mRNA transcriptional changes 11 genes related damage repair detected using real-time quantitative polymerase chain reaction (RT-PCR).Of examined, CDKN1A (cyclin-dependent kinase...
Abstract Hepatitis B surface antigen (HBsAg) and anti‐HBs antibodies (anti‐HBs) may coexist in certain chronic hepatitis (CHB) patients. This study was designed to further explore the relationship between this coexistence Virus (HBV) preS deletions. Sera of 28 patients carrying both HBsAg (Group I) those another positive but negative II) were collected from CHB Direct sequencing polymerase chain reaction products or clones applied groups determine sequences HBV S genes. Genotyping gene...
A balance of inhibitory and activating signals determines the function dendritic cells (DCs) in immune response, which may be regulatory or stimulatory. Defects receptor FcγRIIb are involved pathogenesis autoimmune diseases such as systemic lupus erythematosus (SLE), high levels circulating complexes (IC) exist. Our previous study showed that IC/Ig can suppress TLR4-triggered inflammatory responses macrophages via FcγRIIb. This led us to question whether polarize FcγRIIb-overexpressing DCs...
Abstract This study was designed to detect and analyze mutations that occur within the presurface surface (pre‐S/S) gene of HBV in patients with occult hepatitis B, determine their relationship disorder. Among 254 HBsAg negative samples blood collected eastern China, 183 were positive for anti‐HBc alone, 61 anti‐HBe 10 HBeAg. Within this group, 15 found be DNA by real‐time PCR designated Group I. A control group 28 chosen at random from chronic B II. The pre‐S/S amplified subjected...
Multiple myeloma (MM) is a type of hematological malignancy that can affect all types tissues in human. However, it extremely rare pleural effusion presents as the first sign MM patients. A 54-year-old male patient attended our department respiratory medicine complaining shortness breath for past 3 months. chest computer tomography (CT) radiograph revealed bilateral effusion, which was further assessed exudative type. Sinus spiral CT scan demonstrated diffuse bone destruction craniofacial...
Summary Aquaporin 5 (AQP5) is one of the water channel proteins which participate in a wide array physiological processes and are primary determinants membrane osmotic permeability. The AQP5 gene located human chromosome 12q, same region as location major asthma susceptibility loci. In this study we try to determine whether knock-out has some effect on allergen-induced asthma. With mouse model induced by ovalbumin (OVA), found that deletion reduced characteristic features asthma, such less...